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Team:Alberta/Notebook
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| - | <font size=5> iGem | + | <font size=5> iGem Notebook</font> |
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| - | Rick and Tom | + | Rick and Tom began coming in to the lab regularly and started learning basic laboratory protocols, such as making competent cells, restriction digests, cell transformation, gel electrophoresis, ect. |
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Today Tom finally managed to get a PCR to work, though it took him about twelve attempts. | Today Tom finally managed to get a PCR to work, though it took him about twelve attempts. | ||
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| + | |[[https://2012.igem.org/Team:Alberta/Notebook Top page]] | ||
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| - | Competent cells were made today using a standard procedure which took the entire day. | + | Competent cells were made today using a standard procedure which took the entire day. The competent cell procedure is described elsewhere in this wiki. |
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| - | The competent cells were tested with basic puc19 transformation, and the transformation worked | + | The competent cells were tested with basic puc19 transformation, and the transformation worked with acceptable efficiency. |
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| - | Today multiple PCRs were run on puc19 with two of the color genes and a | + | Today multiple PCRs were run on puc19 with two of the color genes and a repressor gene. |
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| - | Made pUC19 plasmid with | + | Made pUC19 plasmid with various color genes and C1 and transformed it into cells. |
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| - | We cloned a variety of different ( | + | We cloned a variety of different promoters (nine total) into our color gene plasmids in order to get a sense of relative promoter strengths. We also sent off to sequencing those construct to check that they are correct. |
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| - | We PCR new RBS colour genes and regulatory promoters. | + | We used PCR to add new RBS colour genes and regulatory promoters to our constructs. We then pieced those constructs into kanamycin resistant plasmid backbone and transform them into Top 10 cells. |
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| + | |[[https://2012.igem.org/Team:Alberta/Notebook Top page]] | ||
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| - | <div class="underline"><font size=5> | + | <div class="underline"><font size=5>July</font></div> |
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| - | Torrin and Sarah | + | Torrin and Sarah join the team. We performed the same work on the TG1 strain of E.Coli as a learning exercise. |
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| - | + | Creation of a plasmid with a cut site near the origin of replication began today. This plasmid will be used in order to modify the promoter on the plasmid's origin of replication. | |
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Today Tom Used the successful origin cut site plasmid with Amp Resistance and replaced that resistance with Chlr. | Today Tom Used the successful origin cut site plasmid with Amp Resistance and replaced that resistance with Chlr. | ||
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Latest revision as of 21:56, 18 September 2012
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iGem Notebook
May.9-11.2012
Rick and Tom began coming in to the lab regularly and started learning basic laboratory protocols, such as making competent cells, restriction digests, cell transformation, gel electrophoresis, ect.
May. 24
Rick, Tom, Spencer, and Easwar learned how to use PCR today, with varying degrees of success.
May 31
Today Tom finally managed to get a PCR to work, though it took him about twelve attempts.
| [Top page] |
June 4
Made working stock of puc19 and then transformed Top10 with puc19. Overnight cultures were also set up in preparation for the making of competent cells
June 5
Competent cells were made today using a standard procedure which took the entire day. The competent cell procedure is described elsewhere in this wiki.
June 6
The competent cells were tested with basic puc19 transformation, and the transformation worked with acceptable efficiency.
June 7-8
Today multiple PCRs were run on puc19 with two of the color genes and a repressor gene.
June 11
Made pUC19 plasmid with various color genes and C1 and transformed it into cells.
June 18-21
We cloned a variety of different promoters (nine total) into our color gene plasmids in order to get a sense of relative promoter strengths. We also sent off to sequencing those construct to check that they are correct.
June 22- July 3
We used PCR to add new RBS colour genes and regulatory promoters to our constructs. We then pieced those constructs into kanamycin resistant plasmid backbone and transform them into Top 10 cells.
| [Top page] |
July 4-5
Torrin and Sarah join the team. We performed the same work on the TG1 strain of E.Coli as a learning exercise.
July 6
Creation of a plasmid with a cut site near the origin of replication began today. This plasmid will be used in order to modify the promoter on the plasmid's origin of replication.
July 9-10
Today Tom Used the successful origin cut site plasmid with Amp Resistance and replaced that resistance with Chlr.
