Team:Wageningen UR/Journal/week8

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= week 8: 18 june - 24 june =
= week 8: 18 june - 24 june =
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The DNA of our Polero virus finally arrived! It turns out not to be the Potato Leafroll Virus (PLRV), but instead the Turnip Yellows Virus (TuYV) which is a close relative (also from the Polero genus).
 
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== Office work ==
 
== Lab work ==
== Lab work ==
'''TuYV'''
'''TuYV'''
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<p align="justify">
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The DNA of our Polero virus finally arrived! It turns out not to be the Potato Leafroll Virus (PLRV), but instead the Turnip Yellows Virus (TuYV) which is a close relative (Polero and TuYV both are from genus ''Polerovirus'' and family Luteoviridae).
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</p>
Friday:
Friday:
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*6 different Coat Protein (CP) constructs were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and a Histidine tag.
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*With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthrough, later they are named 1,1H,2,2H,3,3H.  
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[[File:P12 PCR amplification of 1,1H, 2, 2H, 3, 3H. .jpg|center|thumb|500px|Figure 1: Isolation TuYV constructs 1,1H,2,2H,3,3H from the TuYV genome]]
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Written by: Han Yue
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'''CCMV'''
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Written by: Wouter
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*Digestion and ligation of delta 25 CCMV and CCMV into pSB1K3
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*New PCR amplifications of all fragments for pJET
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]]          [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]]
[[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]]          [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]]

Latest revision as of 23:01, 26 September 2012

week 8: 18 june - 24 june

Lab work

TuYV

The DNA of our Polero virus finally arrived! It turns out not to be the Potato Leafroll Virus (PLRV), but instead the Turnip Yellows Virus (TuYV) which is a close relative (Polero and TuYV both are from genus Polerovirus and family Luteoviridae).

Friday:

  • With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthrough, later they are named 1,1H,2,2H,3,3H.


Figure 1: Isolation TuYV constructs 1,1H,2,2H,3,3H from the TuYV genome


Written by: Han Yue

CCMV

  • Digestion and ligation of delta 25 CCMV and CCMV into pSB1K3
  • New PCR amplifications of all fragments for pJET

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