Team:SJTU-BioX-Shanghai/A

From 2012.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
-
{{Template:12SJTU_header}}
+
{{Template:12SJTU_main_header}}
-
 
+
<html>
-
<html>
+
-
<head>
+
-
<link rel="stylesheet" href="http://igem.bio-x.cn/home/style/contentslider.css" type="text/css"/>
+
-
<script src="http://igem.bio-x.cn/home/style/contentslider.js" type="text/javascript" ></script>
+
-
<style type="text/css">
+
-
#toc, .toc {
+
-
border: 0px;
+
-
 
+
-
padding: 2px;
+
-
 
+
-
}
+
-
</style>
+
-
</head>
+
<body>
<body>
-
<table class="mainouter" width="982" cellspacing="0" cellpadding="5" align="center">
+
<!--      =============================================================================== -->
 +
 +
<table class="mainouter" width="1012" cellspacing="0" cellpadding="5" align="center">
<tbody>
<tbody>
 +
<tr><td class="pumpkin">
 +
</td></tr>
<tr><td class="text" align="center">
<tr><td class="text" align="center">
-
<table class="main" width="940" border="0" cellspacing="0" cellpadding="0"><tbody>
+
<table class="main" width="1010" border="0" cellspacing="0" cellpadding="0"><tbody>
<tr><td class="embedded">
<tr><td class="embedded">
<div>
<div>
-
<ul class="semiopaquemenu">
+
<ul class="semiopaquemenu navipages" id="lavalamp">
-
<li><a href="/Team:SJTU-BioX-Shanghai" class="selected">Home</a></li>
+
<li class="current"><a href="/Team:SJTU-BioX-Shanghai" class="selected">Home</a></li>
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project">Project</a>
+
<li><a href="/Team:SJTU-BioX-Shanghai/Project">Project</a></li>
-
<div class="menubar1"><ul class="semiopaquemenu2">
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project" >Overview</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project/project1" >Membrane Protein</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project/project2" >Accelerater</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project/project3" >Switch</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Project/model" >Modeling</a></li>
+
-
</ul></div>
+
-
</li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Data">Data</a></li>
+
<li><a href="/Team:SJTU-BioX-Shanghai/Parts">Parts</a></li>
<li><a href="/Team:SJTU-BioX-Shanghai/Parts">Parts</a></li>
-
<li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a>
+
<li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a></li>
-
<div class="menubar2"><ul class="semiopaquemenu2">
+
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a></li>
-
<li><a href="/Team:SJTU-BioX-Shanghai/Team" >Overview</a><br></li>
+
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a></li>
-
<li><a href="/Team:SJTU-BioX-Shanghai/Team/members" >Team Membrers</a><br></li>
+
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/safety">Consideration</a></li>
-
<li><a href="/Team:SJTU-BioX-Shanghai/Team/Gallary" >Gallary</a><br></li>
+
-
</ul></div>
+
-
</li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook">Notebook</a>
+
-
<div class="menubar3"><ul class="semiopaquemenu2">
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook/progress" >Labwork Progress</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook/log" >Lablog</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol" >Protocol</a><br></li>
+
-
</ul></div>
+
-
</li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration">Consideration</a>
+
-
<div class="menubar4"><ul class="semiopaquemenu2">
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human" >Human Practice</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/safety" >Safty</a><br></li>
+
-
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/attr" >Attributions</a><br></li>
+
-
</ul></div>
+
-
</li>
+
</ul>
</ul>
</div>
</div>
Line 62: Line 27:
</td></tr>
</td></tr>
-
<tr><td align="center" class="outer" style="padding-top: 20px; padding-bottom: 20px">
+
<tr><td align="center" class="outer">
-
<table width="970" class="main" border="0" cellspacing="0" cellpadding="0"><tbody>
+
<table width="1010" class="main" border="0" cellspacing="0" cellpadding="0"><tbody>
-
<tr><td align="left"> <h2><b>Membrane Workshop</b></h2>
+
<tr><!-- abstract -->
-
 
+
<td>
-
</td></tr>
+
<div id="abstract">
-
<table border="0" cellspacing="0" cellpadding="0" align="center"><tbody>
+
<div id="abstract-title"></div>
-
<tr>
+
<div id="abstract-content">
-
<td valign="top" width="500">
+
<p>This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled. </p>
-
<div align="left">
+
<p>We aimed at constructing a set of protein assemblies on E.coli cell membrane as carriers of various enzymes. Distinct from previous synthetic scaffold system, our device limits the reaction space to a two-dimensional surface. In such system, the membrane functions as an extensive scaffold for proteins to anchor without limitation of scaffold amount. More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device. </p>
-
 
+
<p>By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of fatty acid was enhanced by more than 24 fold through recruiting our membrane accelerator system, which has a promising application prospect in biofuel production.</p>
-
<p>Our slogan: Cluster makes it faster; interaction alters the direction</p>
+
<p>In ''Membrane Rudder'' device, we successfully changed the direction of Violacein synthetic pathway through light signal. </p>
-
<b>Motivation</b><br>
+
</div>
-
<ol><li>There lacks compartment in prokaryotic cells, and thus engineered enzymes diffuse in the cytoplasm, which makes certain reactions proceed at a very low speed.  
+
-
</li><li>Divergent biochemical pathways commonly exist in all types of organisms, but it is extremely hard to artificially control and switch the directions of these reactions.
+
-
</li></ol>
+
-
<b>What to do</b><br>
+
-
<p>We aim to attach enzymes involved in certain reactions to membrane proteins in order to fulfill goals stated below:</p>
+
-
<ol><li>To accelerate reactions in certain biochemical pathway
+
-
</li><li>To switch the biochemical pathway from one to the other through extracellular signal control
+
-
</li></ol>
+
-
</div>
+
-
<td valign="top">
+
-
<!--webbot bot="HTMLMarkup" startspan -->
+
-
<div id="slider4" class="sliderwrapper">
+
-
 
+
-
<div class="contentdiv" style="background-image: url(https://2012.igem.org/File:Yinruonan.png); display: block; z-index: 4; opacity: 1.0999999999999999; visibility: visible; background-position: 0% 50%; background-repeat: no-repeat no-repeat; ">
+
</div>
</div>
-
 
+
</td>
-
<div class="contentdiv" style="background-image: url(/wiki/images/c/cb/12SJTU_slidercontent2.jpg); display: none; z-index: 3; opacity: 0.7; visibility: visible; background-position: 0% 50%; background-repeat: no-repeat no-repeat; ">
+
</tr>
 +
 +
<tr><!-- design of membrane system-->
 +
<td style="border-top-width:40px;">
 +
<div id="design">
 +
<div id="design-title"></div>
 +
<div id="design-content">
 +
</div>
</div>
</div>
-
 
+
</td>
-
<div class="contentdiv" style="background-image: url(/wiki/images/7/79/12SJTU_slidercontent3.jpg); display: none; z-index: 2; opacity: 0.4; visibility: visible; background-position: 0% 50%; background-repeat: no-repeat no-repeat; ">
+
</tr>
 +
 +
<tr><!-- 3 entrys -->
 +
<td style="border-top-width:20px;" align="center">
 +
<div class="main-entry" style="background-color:#ffb000; margin-right:26px;">
 +
<div class="entry-title">
 +
<img src="/wiki/images/d/d9/12SJTU_title_accelerator.png">
</div>
</div>
-
 
+
<div class="entry-content">
-
<div class="contentdiv" style="background-image: url(/wiki/images/5/51/12SJTU_slidercontent4.jpg); display: none; z-index: 1; opacity: 1.0999999999999999; visibility: visible; background-position: 0% 50%; background-repeat: no-repeat no-repeat; ">
+
</div>
</div>
-
 
</div>
</div>
-
 
+
<div class="main-entry" style="background-color:#4fb1ca; margin-right:26px;">
-
<div id="paginate-slider4" style="background:white">
+
<div class="entry-title">
-
 
+
<img src="/wiki/images/5/5b/12SJTU_title_rudder.png">
-
<a href="" class="toc someclass selected" style="margin-left: 35px" rel="1">
+
</div>
-
<img src="/wiki/images/4/47/12SJTU_slidercontent1_thumb.jpg"></a>  
+
<div class="entry-content">
-
<a href="" class="toc someclass" rel="2">
+
</div>
-
<img src="/wiki/images/3/3f/12SJTU_slidercontent2_thumb.jpg"></a>  
+
</div>
-
<a href="" class="toc someclass" rel="3">
+
<div class="main-entry" style="background-color:#fe88b8;">
-
<img src="/wiki/images/8/82/12SJTU_slidercontent3_thumb.jpg"></a>  
+
<div class="entry-title">
-
<a href="" class="toc someclass" rel="4">
+
<img src="/wiki/images/c/cc/12SJTU_title_decomposer.png">
-
<img src="/wiki/images/4/4a/12SJTU_slidercontent4_thumb.jpg"></a>
+
</div>
-
 
+
<div class="entry-content">
 +
</div>
</div>
</div>
-
 
-
<script type="text/javascript">
 
-
 
-
featuredcontentslider.init({
 
-
id: "slider4",  //id of main slider DIV
 
-
contentsource: ["inline", ""],  //Valid values: ["inline", ""] or ["ajax", "path_to_file"]
 
-
toc: "markup",  //Valid values: "#increment", "markup", ["label1", "label2", etc]
 
-
nextprev: ["", "Next"],  //labels for "prev" and "next" links. Set to "" to hide.
 
-
revealtype: "mouseover", //Behavior of pagination links to reveal the slides: "click" or "mouseover"
 
-
enablefade: [true, 0.1],  //[true/false, fadedegree]
 
-
autorotate: [true, 3000],  //[true/false, pausetime]
 
-
onChange: function(previndex, curindex){  //event handler fired whenever script changes slide
 
-
//previndex holds index of last slide viewed b4 current (1=1st slide, 2nd=2nd etc)
 
-
//curindex holds index of currently shown slide (1=1st slide, 2nd=2nd etc)
 
-
}
 
-
})
 
-
 
-
</script><!--webbot bot="HTMLMarkup" endspan i-checksum="36125" -->
 
-
</td> </tr>
 
-
</tbody></table>
 
-
<table border="0" cellspacing="0" cellpadding="0" align="left"><tbody>
 
-
<tr>
 
-
<td>
 
-
<h2><b>Membrane Engine (Accelerator)</b></h2>
 
-
<p>As there is no compartment in prokaryotic cells, enzymes involved in a biochemical pathway diffuse all over the cytoplasm. Intermediates generated from one enzyme cannot be passed efficiently to the next due to spatial obstacles.  However, if we attach those enzymes to engineered membrane proteins which assemble together, the reactions are going to proceed much faster. </p>
 
-
<p><i>Why do we attach enzymes to interacting protein domains and ligands that assemble together?</i> </p>
 
-
<ul><li>Interacting proteins fused with enzymes can decrease the distance which intermediates must travel between enzymes, improving reaction speed. 
 
-
</li></ul>
 
-
<p><i>Why do we localize the enzymes to the membrane?</i></p>
 
-
<ul><li>Interaction of proteins can only be effective within a small distance. Membrane localization of the enzymes can integrate those engineered proteins to 2-dimensional scale, which would absolutely increase the possibility that potential interacting domains and ligands dimerize.
 
-
</li></ul>
 
-
<h2><b>Membrane Switch</b></h2>
 
-
<p>Now that we have built a device that can speed up a biological pathway. Our next goal is to control the pathway better---- to switch the direction of certain reactions, as shown in figure 3.</p>
 
-
<p>Divergent biochemical pathways commonly exist in all types of organisms, and most of those reactions are stringently and internally controlled. However, it is extremely hard to artificially control and switch the directions of these reactions. Usually there are two different products produced in divergent reactions. Sometimes we want one product, and sometimes we want the other. Using our designed device, we can change the direction by introducing different extracellular signals. </p>
 
</td>
</td>
</tr>
</tr>
-
</tbody></table>
+
-
<!--!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!-->
+
<tr><!-- achievements & video -->
 +
<td style="border-top-width:20px;">
 +
<div id="achievements">
 +
<div id="achievements-title"></div>
 +
<div id="achievements-content">
 +
</div>
 +
</div>
 +
<div id="video">
 +
<embed src="http://player.youku.com/player.php/sid/XNDU1MjQyNTQ4/v.swf" allowfullscreen="true" quality="high" width="480" height="400" align="middle" allowscriptaccess="always" type="application/x-shockwave-flash">
 +
</div>
 +
</td></tr>
 +
 +
<tr><td style="border-top-width:40px;">
 +
<div id="sponsors">
 +
<div id="sponsors-title"><div>
 +
</div>
 +
</td></tr>
 +
<!--                     !!!!!!!!!!!!!! -->
</tbody></table>
</tbody></table>
</td></tr>
</td></tr>
</tbody></table>
</tbody></table>
<br>
<br>
 +
 +
 +
<div id="toolBackTo" class="back-to" >
 +
<a class="backtotop" href="#top" onclick="window.scrollTo(0,0);return false;">To Top
 +
 +
</a>
 +
</div>
 +
 +
 +
<div align="center" id="footer"> (c)  <a href="" target="_self">SJTU_BioX_iGEM2012</a> Powered by huanan1991</div>
<div align="center" id="footer"> (c)  <a href="" target="_self">SJTU_BioX_iGEM2012</a> Powered by huanan1991</div>
 +
</div>
</body>
</body>
</html>
</html>

Latest revision as of 12:39, 18 October 2012

This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled.

We aimed at constructing a set of protein assemblies on E.coli cell membrane as carriers of various enzymes. Distinct from previous synthetic scaffold system, our device limits the reaction space to a two-dimensional surface. In such system, the membrane functions as an extensive scaffold for proteins to anchor without limitation of scaffold amount. More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device.

By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of fatty acid was enhanced by more than 24 fold through recruiting our membrane accelerator system, which has a promising application prospect in biofuel production.

In ''Membrane Rudder'' device, we successfully changed the direction of Violacein synthetic pathway through light signal.


Retrieved from "http://2012.igem.org/Team:SJTU-BioX-Shanghai/A"