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Team:Osaka/week2
From 2012.igem.org
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| - | {{ | + | {{Osaka2}} |
__NOTOC__ | __NOTOC__ | ||
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==August 5 (Sun)== | ==August 5 (Sun)== | ||
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
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# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
| - | # | + | # Transformation of Registry parts into DH5α <i>E.coli</i>(See Table 2) |
# Preparation of glycerol stock of cell culture:143~146,149,152 | # Preparation of glycerol stock of cell culture:143~146,149,152 | ||
| Line 32: | Line 33: | ||
==August 8 (Wed)== | ==August 8 (Wed)== | ||
| - | # Transfer to liquid culture:1-2D,1-10H,1-22A | + | # Transfer to liquid culture:1-2D,1-10H,1-22A,151 |
==August 9 (Thu)== | ==August 9 (Thu)== | ||
| - | + | # Miniprep of yesterday's culture | |
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | #* 1-10H not ok | ||
| + | # Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 3) | ||
| + | # Transformation of plasmid DNA into Rosetta <i>E.coli</i>:147,148,150 | ||
| + | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
| + | |+Table3 | ||
| + | !ID!!Part Name!!Resistance!!Description | ||
| + | |- | ||
| + | |1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS | ||
| + | |} | ||
==August 10 (Fri)== | ==August 10 (Fri)== | ||
| - | + | # Sequencing reaction:143~152 | |
| + | # Sequencing purification | ||
| + | # Transfer to liquid culture:1-2M,147,148,150 | ||
| + | # Transformation of Registry parts into DH5α <i>E.coli</i>:1-10H | ||
==August 11 (Sat)== | ==August 11 (Sat)== | ||
| - | + | # Preparation of glycerol stock of cell culture:147,148,150 | |
| + | # Miniprep of yesterday's culture:1-2M | ||
| + | # Restriction digests of minipreppped parts | ||
| + | # Transfer to liquid culture:1-10H | ||
| + | # SDS-PAGE :149,150,151,152,empty vector | ||
| + | #* IPTG induction | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
Latest revision as of 01:30, 14 September 2012
August 5 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transfer to liquid culture
- (Rosetta)143~146,149,152
- (DH5α)150,Empty vector(A)
August 6 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformation of Registry parts into DH5α E.coli(See Table 2)
- Preparation of glycerol stock of cell culture:143~146,149,152
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-2D | <bbpart>BBa_J52008</bbpart> | A,K | Renilla Luciferase |
| 1-10H | <bbpart>BBa_I712019</bbpart> | A,K | Firefly Luciferase from Photinus pyralis |
| 1-22A | <bbpart>BBa_J23118</bbpart> | A | Constitutive promoter family member |
August 7 (Tue)
- Preparation of LB agar plates (Cam)
- Transformation of 1-2D,1-10H
August 8 (Wed)
- Transfer to liquid culture:1-2D,1-10H,1-22A,151
August 9 (Thu)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 1-10H not ok
- Transformaton of Registry parts into DH5α E.coli(See Table 3)
- Transformation of plasmid DNA into Rosetta E.coli:147,148,150
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-2M | <bbpart>BBa_K118022</bbpart> | A | RBS |
August 10 (Fri)
- Sequencing reaction:143~152
- Sequencing purification
- Transfer to liquid culture:1-2M,147,148,150
- Transformation of Registry parts into DH5α E.coli:1-10H
August 11 (Sat)
- Preparation of glycerol stock of cell culture:147,148,150
- Miniprep of yesterday's culture:1-2M
- Restriction digests of minipreppped parts
- Transfer to liquid culture:1-10H
- SDS-PAGE :149,150,151,152,empty vector
- IPTG induction
