Team:Amsterdam

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<title>iGEM Amsterdam: Cell Logbook</title>
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<h2>Cellular Logbook - A methylation-based reporter system</h2>
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'''Multi-sensing genetic devices''' offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative '''epigenetic DNA-methylation''' based '''detection system''' in <i>E. coli</i>, named '''Cellular Logbook''', that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to '''detect (Sensor module) and store (Writer module)''' the presence of '''any compound''' linked to a transcriptional regulator. This system allows for '''offline monitoring''' by functioning as a '''memory module (Writer module)'''. Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis '''(Reader Module)'''. Furthermore, the Cellular Logbook is able to infer the '''time of signal-onset''' or '''signal-intensity''' using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a '''mulit-sensor/time indicating platform''' for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).
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<p>Bacterial populations in their natural form are very capable of storing, processing and acting on stimuli in their environment. Why not exploit these properties in the pursuit of massively parallel computation? The first thing one needs when constructing a computing device is memory to store the results onto. In order to meet this demand we present the cellular logbook which is usable to reliably store whether a signal was detected in E. coli's environment for a time span of its life duration. Expanding this straightforward molecular mechanism, multiple signals can be stored with the prospective of storing up to 64 bits on a single plasmid. Complex cellular behaviour can then be programmed according to the memory status of the cell by way of introducing additional modules transcriptionally controlled by the introduced memory plasmid.</p>
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<h2>Sponsors</h2>
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<a href="http://www.aimms.vu.nl/en/index.asp"><img src="https://static.igem.org/mediawiki/2012/1/12/Amsterdam_Logo_AIMMS.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.sysbio.nl/"><img src="https://static.igem.org/mediawiki/2012/0/01/Amsterdam_Logo_NISB.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.uva-alumni.nl/page.aspx?pid=460"><img src="https://static.igem.org/mediawiki/2012/3/3f/Amsterdam_Logo_universiteitfonds.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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Latest revision as of 03:56, 27 September 2012

Cellular Logbook - A methylation-based reporter system

Multi-sensing genetic devices offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative epigenetic DNA-methylation based detection system in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to detect (Sensor module) and store (Writer module) the presence of any compound linked to a transcriptional regulator. This system allows for offline monitoring by functioning as a memory module (Writer module). Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis (Reader Module). Furthermore, the Cellular Logbook is able to infer the time of signal-onset or signal-intensity using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a mulit-sensor/time indicating platform for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).

Sponsors

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