Team:Technion/7 August 2012
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==Asaf== | ==Asaf== | ||
+ | I have started on the project of fusing the RiboSwitch with the different RNA polymerases.<br> | ||
+ | Today I have made a starter of Puc19 12.1 with the Riboswitch from the glycerol stock. | ||
==Hila== | ==Hila== | ||
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==Lior== | ==Lior== | ||
- yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.<br> | - yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.<br> | ||
- | - | + | - after consulting with Roee me and Noa decided that we need one more approach for the test plasmids: using xyIE reporter gene from the distribution kit |
- | + | so i did transformation to TopTenRB compotent cells and plated 100 and 200 ul on an Amp plate. | |
==Noa== | ==Noa== | ||
Latest revision as of 07:10, 9 August 2012
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Ilya
- This morning I came up with a few improvements for our strategy for dividing the phage into workable fragments. I presented them to Hila, who also did some research and we presented everything to Roee. Looks like this idea might actually work.
- We had a group meeting, in which we all presented what we are planing to do.
- I did some preparation for the plate reader experiment tomorrow. Prepared BA and theiphylline stocks. Planned the dilutions and the detailed protocol.
- Put a 5ml starter of pSB1C3+Fus2 for the plate reader tomorrow.
- Put a 10ml starter of pSB1A2+F2620 for minipreps tomorrow.
Inbal
Asaf
I have started on the project of fusing the RiboSwitch with the different RNA polymerases.
Today I have made a starter of Puc19 12.1 with the Riboswitch from the glycerol stock.
Hila
Lior
- yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.
- after consulting with Roee me and Noa decided that we need one more approach for the test plasmids: using xyIE reporter gene from the distribution kit
so i did transformation to TopTenRB compotent cells and plated 100 and 200 ul on an Amp plate.