Team:RHIT/Notebook
From 2012.igem.org
(55 intermediate revisions not shown) | |||
Line 14: | Line 14: | ||
$('#logged_in').show(); | $('#logged_in').show(); | ||
} | } | ||
+ | $('#rhit-sheet2').hide(); | ||
+ | var split = location.search.replace('?', '').split('='); | ||
+ | window[split[1]](); | ||
}); | }); | ||
function page1() { | function page1() { | ||
Line 19: | Line 22: | ||
$('#rhit-redSheet').hide(); | $('#rhit-redSheet').hide(); | ||
$('#rhit-grnSheet').hide(); | $('#rhit-grnSheet').hide(); | ||
+ | $('#rhit-fourthSheet').hide(); | ||
} | } | ||
function page2() { | function page2() { | ||
Line 24: | Line 28: | ||
$('#rhit-redSheet').show(); | $('#rhit-redSheet').show(); | ||
$('#rhit-grnSheet').hide(); | $('#rhit-grnSheet').hide(); | ||
+ | $('#rhit-fourthSheet').hide(); | ||
} | } | ||
function page3() { | function page3() { | ||
Line 29: | Line 34: | ||
$('#rhit-redSheet').hide(); | $('#rhit-redSheet').hide(); | ||
$('#rhit-grnSheet').show(); | $('#rhit-grnSheet').show(); | ||
+ | $('#rhit-fourthSheet').hide(); | ||
+ | } | ||
+ | function page4() { | ||
+ | $('#rhit-blueSheet').hide(); | ||
+ | $('#rhit-redSheet').hide(); | ||
+ | $('#rhit-grnSheet').hide(); | ||
+ | $('#rhit-fourthSheet').show(); | ||
} | } | ||
</script> | </script> | ||
Line 35: | Line 47: | ||
<div class="rhit-content"> | <div class="rhit-content"> | ||
<div class="rhit-header"> | <div class="rhit-header"> | ||
- | <img src="/ | + | <a href="https://2012.igem.org/Team:RHIT"><img src="https://static.igem.org/mediawiki/igem.org/8/87/Banner_only2.png" width="100%"/></a> |
<div id="logged_out"> | <div id="logged_out"> | ||
+ | <a href="https://2012.igem.org/Main_Page">iGEM Home</a> | ||
<a href="https://2012.igem.org/wiki/index.php?title=Special:UserLogin&returnto=2012.igem.org/Team:RHIT">Login</a> | <a href="https://2012.igem.org/wiki/index.php?title=Special:UserLogin&returnto=2012.igem.org/Team:RHIT">Login</a> | ||
</div> | </div> | ||
- | + | <div id="logged_in"> | |
- | + | <a href="https://2012.igem.org/Main_Page">iGEM Home</a> | |
- | + | <a id='user-link' class='RHIT-external' href='#'></a> | |
- | + | <a id='account-link' class='RHIT-external' href='https://igem.org/User_Information'>My Account</a> | |
- | + | <a id='logout-link' class='RHIT-external' href='https://2012.igem.org/wiki/index.php?title=Special:UserLogout&returnto=2012.igem.org/Team:RHIT'>Log Out</a> | |
+ | </div> | ||
</div> | </div> | ||
<div class="rhit-navLeft"> | <div class="rhit-navLeft"> | ||
Line 80: | Line 94: | ||
<div class="rhit-grnDivider"> | <div class="rhit-grnDivider"> | ||
Lab Protocols | Lab Protocols | ||
+ | </div> | ||
+ | </a> | ||
+ | <a href="javascript:void(0)" onclick="page4()"> | ||
+ | <div class="rhit-fourthDivider"> | ||
+ | Safety | ||
</div> | </div> | ||
</a> | </a> | ||
Line 86: | Line 105: | ||
<div class="rhit-blueSheet" id="rhit-blueSheet"> | <div class="rhit-blueSheet" id="rhit-blueSheet"> | ||
<h4>Week 1</h4> | <h4>Week 1</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>6/4/2012: First round of project planning, lab clean-up, general outline for summer.</li><br /> | <li>6/4/2012: First round of project planning, lab clean-up, general outline for summer.</li><br /> | ||
<li>6/5/2012: Second round of project planning, lab clean-up, research into Johns Hopkins 2008 summer project.</li><br /> | <li>6/5/2012: Second round of project planning, lab clean-up, research into Johns Hopkins 2008 summer project.</li><br /> | ||
Line 94: | Line 113: | ||
</ul> | </ul> | ||
<h4>Week 2</h4> | <h4>Week 2</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>6/11/2012: Identified a positive feedback loop that's been done in yeast before and a possible selection scheme, started plasmid mapping, set up server for wiki, began wiki design and looked at color schemes, block-diagrammed positive feedback loop, ate a whole tray of brownies.</li><br /> | <li>6/11/2012: Identified a positive feedback loop that's been done in yeast before and a possible selection scheme, started plasmid mapping, set up server for wiki, began wiki design and looked at color schemes, block-diagrammed positive feedback loop, ate a whole tray of brownies.</li><br /> | ||
<li>6/12/2012: Found gene sequences, continued work on graphic for top of Wiki, looked for selection schemes, shot down selection schemes.</li><br /> | <li>6/12/2012: Found gene sequences, continued work on graphic for top of Wiki, looked for selection schemes, shot down selection schemes.</li><br /> | ||
Line 102: | Line 121: | ||
</ul> | </ul> | ||
<h4>Week 3</h4> | <h4>Week 3</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>6/18/2012: Optimized DNA sequences, found all of the filler DNA.</li><br /> | <li>6/18/2012: Optimized DNA sequences, found all of the filler DNA.</li><br /> | ||
<li>6/19/2012: Talked with Dr. Almaas, started web design, took pictures with storm troopers, team movie night.</li><br /> | <li>6/19/2012: Talked with Dr. Almaas, started web design, took pictures with storm troopers, team movie night.</li><br /> | ||
Line 110: | Line 129: | ||
</ul> | </ul> | ||
<h4>Week 4</h4> | <h4>Week 4</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>6/25/2012: Group discussion, work on school website, organization of collected information.</li><br /> | <li>6/25/2012: Group discussion, work on school website, organization of collected information.</li><br /> | ||
<li>6/26/2012: Obtained shirts, found and organized all sequences, started to verify sequences, school page is 85% done.</li><br /> | <li>6/26/2012: Obtained shirts, found and organized all sequences, started to verify sequences, school page is 85% done.</li><br /> | ||
Line 119: | Line 138: | ||
<h4>Team Break Week</h4> | <h4>Team Break Week</h4> | ||
<h4>Week 5</h4> | <h4>Week 5</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>7/9/2012: Got back from break. Learned DNA had not shipped yet. :( Worked on wiki.</li><br /> | <li>7/9/2012: Got back from break. Learned DNA had not shipped yet. :( Worked on wiki.</li><br /> | ||
<li>7/10/2012: Worked on wiki.</li><br /> | <li>7/10/2012: Worked on wiki.</li><br /> | ||
Line 127: | Line 146: | ||
</ul> | </ul> | ||
<h4>Week 6</h4> | <h4>Week 6</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>7/16/2012: Decided to use GeneArt for sequences instead of original DNA company.</li><br /> | <li>7/16/2012: Decided to use GeneArt for sequences instead of original DNA company.</li><br /> | ||
<li>7/18/2012: Brainstormed ideas for side projects while waiting for DNA shipment.</li><br /> | <li>7/18/2012: Brainstormed ideas for side projects while waiting for DNA shipment.</li><br /> | ||
Line 135: | Line 154: | ||
</ul> | </ul> | ||
<h4>Week 7</h4> | <h4>Week 7</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>7/23/2012: Devon and Kristen began planning the THCM exhibit.</li><br /> | <li>7/23/2012: Devon and Kristen began planning the THCM exhibit.</li><br /> | ||
<li>7/24/2012: Continued work in Teams. Began intial research into pathway for modeling.</li><br /> | <li>7/24/2012: Continued work in Teams. Began intial research into pathway for modeling.</li><br /> | ||
Line 143: | Line 162: | ||
</ul> | </ul> | ||
<h4>Week 8</h4> | <h4>Week 8</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>7/30/2012: Ironed out wiki formatting, began adding content, continued Maya animation.</li><br /> | <li>7/30/2012: Ironed out wiki formatting, began adding content, continued Maya animation.</li><br /> | ||
<li>7/31/2012: Lab work, continued adding content to wiki.</li><br /> | <li>7/31/2012: Lab work, continued adding content to wiki.</li><br /> | ||
<li>8/1/2012: Lab work, added more content to wiki, got update on additional computer for Maya animation, made significant progress on math model, Rose-Hulman team page went live.</li><br /> | <li>8/1/2012: Lab work, added more content to wiki, got update on additional computer for Maya animation, made significant progress on math model, Rose-Hulman team page went live.</li><br /> | ||
<li>8/2/2012: Lab work, continued work in Teams.</li><br /> | <li>8/2/2012: Lab work, continued work in Teams.</li><br /> | ||
- | <li>8/3/2012: Lab work, continued work in Teams.</li | + | <li>8/3/2012: Lab work, continued work in Teams.</li> |
</ul> | </ul> | ||
<h4>Week 9</h4> | <h4>Week 9</h4> | ||
- | <ul id="dailyLog | + | <ul id="dailyLog"> |
<li>8/6/2012: Canoe trip with IRC members, lab work.</li><br /> | <li>8/6/2012: Canoe trip with IRC members, lab work.</li><br /> | ||
<li>8/7/2012: Lab work, focus on modeling information for NTNU, continued work in Teams.</li><br /> | <li>8/7/2012: Lab work, focus on modeling information for NTNU, continued work in Teams.</li><br /> | ||
+ | <li>8/8/2012: Lab work, Second Skype meeting with NTNU, conclusion and review of Maya animation.</li><br /> | ||
+ | <li>8/9/2012: Lab work, continued work in Teams.</li><br /> | ||
+ | <li>8/10/2012: Lab work, continued work in Teams.</li> | ||
+ | </ul> | ||
+ | <h4>Week 10</h4> | ||
+ | <ul id="dailyLog"> | ||
+ | <li>8/13/2012: Lab work, continued work in Teams.</li><br /> | ||
+ | <li>8/14/2012: Lab work, continued work in Teams.</li><br /> | ||
+ | <li>8/15/2012: Lab work, continued work in Teams.</li><br /> | ||
+ | <li>8/16/2012: Lab work, plans made for continuing into the school year.</li><br /> | ||
+ | <li>8/17/2012: Cleaned workspace, finalized plans for continuation.</li><br /> | ||
</ul> | </ul> | ||
</div></a> | </div></a> | ||
Line 167: | Line 197: | ||
Members: Ben, Bobby, Alex, Kristen, Devon, Dr. A. | Members: Ben, Bobby, Alex, Kristen, Devon, Dr. A. | ||
<h3>August 3, 2012</h3> | <h3>August 3, 2012</h3> | ||
- | Qiagen minipreps | + | Qiagen minipreps<br /> |
Members: Ben, Bobby, Alex, Kristen, Devon, Dr. Dave. | Members: Ben, Bobby, Alex, Kristen, Devon, Dr. Dave. | ||
+ | <h3>August 6, 2012</h3> | ||
+ | Made stock solutions for transformation procedures<br /> | ||
+ | Members: Adam, Kristen, Dr. A | ||
+ | <h3>August 7, 2012</h3> | ||
+ | Transformed E. coli with our red construct<br /> | ||
+ | Members: Bobby, Adam, Devon, Kristen, Ben, Dr. A | ||
+ | <h3>August 8, 2012</h3> | ||
+ | Inoculated liquid media with transformed E.Coli<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 9, 2012</h3> | ||
+ | Ran Mini-Preps of pRS413, pRS416, and Red construct bearing E. Coli, stored extracted DNA<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 10, 2012</h3> | ||
+ | Ran quick gel of extracted DNA<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 11, 2012</h3> | ||
+ | Digested extracted DNA, isolated pRS413 & pRS416 fragments from gel<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 12, 2012</h3> | ||
+ | Isolated red construct fragments on gel, poured chloramphenicol plates<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 13, 2012</h3> | ||
+ | Digested extracted DNA, isolated pRS413, pRS416, and Red Construct from gel, Transformed E. Coli with NTNU construct<br /> | ||
+ | Members: Bobby, Adam, Dr. A | ||
+ | <h3>August 14, 2012</h3> | ||
+ | Ligated pRS413 and red construct, transformed E. Coli with ligated plasmid, inoculated liquid media with NTNU E. Coli<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 15, 2012</h3> | ||
+ | Mini-Preps of NTNU construct, confirmed presence on gel, Digested pRS413, pRS416, and Red Construct, poured ampicillin plates<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 16, 2012</h3> | ||
+ | Isolated pRS413, pRS416, and Red Construct from gel, began storing strains, DNA, and equipment<br /> | ||
+ | Members: Bobby, Adam | ||
+ | <h3>August 17, 2012</h3> | ||
+ | Ran digestion of pRS413, pRS416, and Red Construct, stored remaining strains, DNA, and equipment<br /> | ||
+ | Members: Bobby, Adam, Dr. A | ||
+ | <h3>August 28, 2012</h3> | ||
+ | Transformed E.Coli with BBa_E0422 reporter<br /> | ||
+ | Members: Bobby, Adam, Dr. A | ||
+ | <h3>August 29, 2012</h3> | ||
+ | Digested and isolated Red Construct and pRS413 from gel, Inoculated liquid media with BBa_E0422 reporter<br /> | ||
+ | Members: Adam | ||
+ | <h3>August 30, 2012</h3> | ||
+ | Digested pRS413 and Red Construct, Isolated from gel and Ligated, Transformed E. Coli.<br /> | ||
+ | Members: Adam | ||
+ | <h3>August 31, 2012</h3> | ||
+ | Digested pRS413 and Red Construct<br /> | ||
+ | Members: Bobby, Adam, Dr. A | ||
+ | <h3>September 1, 2012</h3> | ||
+ | Started cultures of pRS413 and the Red construct form frozen cultures<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 2, 2012</h3> | ||
+ | Inoculated liquid media with colonies from plates of pRS413 and Red Construct strains<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 3, 2012</h3> | ||
+ | Ran mini-preps of liquid colonies<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 4, 2012</h3> | ||
+ | Ran diagnostic gel to check amount of DNA extracted<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 5, 2012</h3> | ||
+ | Purified and concentrated DNA samples<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 6, 2012</h3> | ||
+ | Ran diagnostic gel to check for purification success<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 7, 2012</h3> | ||
+ | Ran BioBrick Assembly protocol on NTNU lld promoter and BBa_E0422, Transformed E.Coli with ligation mixture<br /> | ||
+ | Members: Adam, Dr. A | ||
+ | <h3>September 8, 2012</h3> | ||
+ | Inoculated liquid cultures with transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 10, 2012</h3> | ||
+ | Digested and isolated Red Construct and pRS413 from gel, Ran mini-preps of liquid cultures<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 11, 2012</h3> | ||
+ | Ran gel of lld/0422 hybrid, Gel showed presence of desired product <br /> | ||
+ | Members: Adam | ||
+ | <h3>September 12, 2012</h3> | ||
+ | Inoculated liquid cultures and plates with transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 13, 2012</h3> | ||
+ | Performed ligation on Red Construct, Transformed E.Coli with blue construct<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 14, 2012</h3> | ||
+ | Inoculated liquid cultures with blue construct transformants<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 15, 2012</h3> | ||
+ | Ran mini-preps on colonies with blue constructs<br /> | ||
+ | Members: Adam and Bobby | ||
+ | <h3>September 16, 2012</h3> | ||
+ | Digested Blue construct and pRS413, isolated desired fragments from gel<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 17, 2012</h3> | ||
+ | Ligated Blue construct and pRS413 fragments, transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 18, 2012</h3> | ||
+ | Inoculated Liquid Media with Blue construct/pRS413 fusion bearing E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 19, 2012</h3> | ||
+ | NTNU construct testing via fluorescence, Mini-Preps of Blue construct/pRS413 fusion bearing E.Coli<br /> | ||
+ | Members: Bobby, Alex, Adam | ||
+ | <h3>September 20, 2012</h3> | ||
+ | Diagnostic gels of Blue Construct/pRS413 mini-preps<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 22, 2012</h3> | ||
+ | Inoculated liquid media with Blue Construct/pRS413 E. Coli, prepared stock solutions<br /> | ||
+ | Members: Adam, Dr. A | ||
+ | <h3>September 23, 2012</h3> | ||
+ | Mini-Preps of E.Coli, Ran gel of prep, made liquid media<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 24, 2012</h3> | ||
+ | Ran digestion of mini-prep DNA, plated Yeast<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 25, 2012</h3> | ||
+ | Gel of digestion result, isolated fragments from gel<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 26, 2012</h3> | ||
+ | Ligated Blue Fragment/pRS413, transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 27, 2012</h3> | ||
+ | Inoculated LB-AMP broth with transformed E.Coli, plated Yeast<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 28, 2012</h3> | ||
+ | Inoculated LB-CARB broth with transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 29, 2012</h3> | ||
+ | Inoculated LB-CARB broth with transformed E.Coli<br /> | ||
+ | Members: Adam | ||
+ | <h3>September 30, 2012</h3> | ||
+ | Performed Mini-preps of transformed E.Coli, Began yeast transformation, plated additional yeast cultures.<br /> | ||
+ | Members: Adam | ||
+ | <h3>October 1, 2012</h3> | ||
+ | Plated Yeast Transformants<br /> | ||
+ | Members: Adam | ||
+ | <h3>October 3, 2012</h3> | ||
+ | Transformed yeast found growing on plates<br /> | ||
+ | Members: Adam | ||
</div> | </div> | ||
<div class="rhit-grnSheet" id="rhit-grnSheet"> | <div class="rhit-grnSheet" id="rhit-grnSheet"> | ||
- | + | <h2>Strains Used</h2> | |
+ | <h3><i>E. Coli</i></h3> | ||
+ | <p>NEB5-alpha (NEB #C2988J or #C2987I): <i>fhuA2 ?(argF-lacZ)U169 phoA glnV44 F80? (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17</i></p><br /> | ||
+ | <h3><i>S. Cerevisiae</i></h3> | ||
+ | <p>BY4741 (ATCC #201388): <i>MATa his3-?0 leu2-?0 met15-?0 ura3-?0</i></p><br /> | ||
+ | <p>BY4742 (ATCC #201389): <i>MATalpha his3-?0 leu2-?0 lys2-?0 ura3-?0</i></p><br /><br /> | ||
+ | <h2>Lab Protocols</h2> | ||
+ | <p>Transformations were carried out on competent E.Coli using the procedure available on the <a href="http://www.neb.com/nebecomm/products/protocol119.asp">NEB website</a>.</p><br /><br /> | ||
+ | |||
+ | <p>DNA extraction from cells was performed using a Qiagen QIAprep Spin Miniprep Kit (Cat. no. 27104). Included procedure was followed.</p><br /><br /> | ||
+ | |||
+ | <p>Digestions were performed using EcoRI, XbaI, SpeI and PstI using recommended NEB procedure.</p><br /><br /> | ||
+ | |||
+ | <p>Gel extraction of digested DNA was performed using a Qiagen QIAEX II Gel Extraction Kit (Cat. no. 20021). Included procedure was followed.</p><br /><br /> | ||
+ | |||
+ | <p>Ligations were performed following procedure available on the <a href="http://www.neb.com/nebecomm/products/protocol658.asp">NEB website</a>. Total mass of DNA for ligations was 100ng with a 1:3 to 1:4 ratio of vector to insert. </p><br /><br /> | ||
+ | |||
+ | </div> | ||
+ | <div class="rhit-fourthSheet" id="rhit-fourthSheet"> | ||
+ | The official RHIT Safety page can be found <a href="https://2012.igem.org/Team:RHIT/Safety">here</a>. | ||
+ | <h2>Researcher, Public, and Environmental Safety</h2> | ||
+ | <p>The Rose-Hulman iGEM team has considered safety a top priority since the early stages of project development. | ||
+ | As a result, projects that could pose a significant risk were dismissed. | ||
+ | Our laboratory space is considered a Basic Biosafety Level 1 laboratory but it does have a few characteristics of higher categories. | ||
+ | It has controlled access, biohazard signs and waste disposal bins, and an autoclave. Other safety features are demonstrated below.<br /></p> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Safety_pics.png" width="60%"></div><br /> | ||
+ | <p>All participating students received standard safety and good laboratory practice training as part of their academic laboratory course-work. | ||
+ | The Instructor provided more specific safety instruction, as necessary. | ||
+ | Proper attire was worn at all times in the laboratory. Gloves and glasses were donned as warranted. | ||
+ | The only organisms used in our project are common laboratory strains of <i>Saccharomyces cerevisiae</i> (BY4741 and BY4742) and <i>Escherichia coli</i> (NEB5alpa), | ||
+ | which are both considered Risk Group 1 microorganisms according to the Laboratory Biosafety Manual published by the World Health Organization. | ||
+ | Group 1 organisms are defined as “no or low individual and community risk, a microorganism that is unlikely to cause human or animal disease.” | ||
+ | Aseptic technique is used whenever working with these organisms and any contaminated wastes are sterilized by autoclaving or destroyed by commercial pyrolysis. | ||
+ | All laboratory chemicals are stored, handled and used as recommended by the manufacturer, | ||
+ | and they are disposed of in accordance with national, state, and local regulations and recommendations. | ||
+ | The laboratory space and contents are not accessible by unauthorized personnel. | ||
+ | All microbial strains, including bacteria rendered antibiotic resistant by transformation, harbor nutritional auxotrophies or other mutations | ||
+ | that mitigate the risk of their growing outside of the laboratory or causing disease in healthy humans or animals. | ||
+ | Furthermore, none of our recombinant constructs produce any known contagion or toxin.<p> | ||
+ | <h2>BioBrick Safety</h2> | ||
+ | <p>None of the BioBrick parts utilized or constructed are known to pose any safety issues. | ||
+ | Furthermore, they are well contained by the microbes that harbor them. The risk of unintended transfer to any other organism is minimal.</p> | ||
+ | <h2>Biosafety Group</h2> | ||
+ | <p>Rose-Hulman does not have a biosafety group, committee or review board other than an Animal Care and Use Committee, | ||
+ | which oversees animal research. Safety training and laboratory waste disposal are facilitated by an Environmental Health and Safety Officer, who also serves as a resource for faculty and students.</p> | ||
+ | <h2>Future iGEM Safety</h2> | ||
+ | <p>The possibility of designing mutually dependent strain/vector systems for routine manipulation and storage of BioBrick parts should be explored.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
- |
Latest revision as of 03:57, 4 October 2012