Lab Journal

April

26/04/2012

We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.

27/04/2012

Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.

May

07/05/2012

Preparing O/N cultures which can be taken out of 37C in the weekend by a friend

08/05/2012

Our friend former igem member Yuri of 2011 will take out our O/N cultures.

09/05/2012

Yuri forgot... Start again. Note; rely on ourselves

10/05/2012

The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.