Team:LMU-Munich/Human Practice/Students Practical Course

From 2012.igem.org

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==Students Practical Course==
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==High School Practical Course==
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On July 27, 28, and 30, we held a '''3-day practical course for 20 students''' from a local high school. The students were 16-18 years old, and had prior knowledge of microbiological principles. For many (or all) students though, the practical course was their first time employing many new techniques.
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On July 27, 28, and 30, we organized a 3-day practical course for 20 students from a local high school. The students were 16-18 years old, and had prior knowledge of microbiological principles. For many (or all) students though, the practical course was their first time employing many new techniques.
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The concept for the practical course was to teach students the principles of synthetic biology, how to work safely in the laboratory, and how to utilize cloning as a biological tool.  
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The concept for the practical course was to teach students the princples and practices of synthetic biology, laboratory safety, and cloning as a biological tool.
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<div class="slideshow" style="height: 488px; width: 650px;">
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*[[File:LMU HS 1.JPG|650px]]
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*[[File:LMU HS 2.JPG|650px]]
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*[[File:LMU HS 3.JPG|650px]]
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*[[File:LMU HS 4.JPG|650px]]
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*[[File:LMU HS 8.JPG|650px]]
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*[[File:LMU HS 10.JPG|650px]]
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*[[File:LMU HS 11.JPG|650px]]
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*[[File:LMU HS 12.JPG|650px]]
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*[[File:LMU HS 13.JPG|650px]]
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*[[File:LMU HS 14.JPG|650px]]
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The daily plan was as follows:
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The daily plan was as follows.
 
'''Friday'''  
'''Friday'''  
Introduction to laboratory safety by Professor Thorsten Mascher
Introduction to laboratory safety by Professor Thorsten Mascher
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Basics of cloning explained by Simon
Basics of cloning explained by Simon
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Dilution series competition
Dilution series competition
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First steps of PCR
First steps of PCR
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'''Saturday'''
'''Saturday'''
Cutting insert with restriction enzymes
Cutting insert with restriction enzymes
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Gel electrophoresis of PCR product
Gel electrophoresis of PCR product
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Presentation
Presentation
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Construction of a new plasmid with RFP
Construction of a new plasmid with RFP
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Transformation of ''E. coli'' with new vectors and plating
Transformation of ''E. coli'' with new vectors and plating
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'''Monday'''
'''Monday'''
Checking plates for red (RFP) ''E. coli'' colonies
Checking plates for red (RFP) ''E. coli'' colonies
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Introduction to synthetic biology by Professor Thorsten Mascher
Introduction to synthetic biology by Professor Thorsten Mascher
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Paper & glue constructs
Paper & glue constructs
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Explanation of the LMU-Munich iGEM 2012 project
Explanation of the LMU-Munich iGEM 2012 project
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Brainstorming and presentation of student '''Sporo'''Bead ideas
Brainstorming and presentation of student '''Sporo'''Bead ideas
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Certificates of participation
Certificates of participation
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</div>
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Have a look at the creative ideas the students had about what to do with our spores:
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<div class="slideshow" style="height: 488px; width: 650px;">
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*[[File:LMU-HP Students 1.jpeg|650px|Pollen-binding for allergic persons]]
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*[[File:LMU-HP Students 2.jpeg|650px|caries-binding]]
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*[[File:LMU-HP Students 3.jpeg|650px|oil-binding]]
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*[[File:LMU-HP Students 3b.jpeg|650px|O2-binding]]
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*[[File:LMU-HP Students 4.jpeg|650px|sense drugs]]
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*[[File:LMU-HP Students 4b.jpeg|650px|NaCl-binding]]
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*[[File:LMU-HP Students 5.jpeg|650px|Lactose-binding]]
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*[[File:LMU-HP Students 5b.jpeg|650px|colour hair]]
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*[[File:LMU-HP Students 6.jpeg|650px|Protein production]]
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*[[File:LMU-HP Students 7.jpeg|650px|wound healing]]
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*[[File:LMU-HP Students 8.jpeg|650px|destroy chitin]]
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*[[File:LMU-HP Students 9.jpeg|650px|Suicide spores]]
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</div>
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<p></p>
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'''Our thoughts'''
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[[File:LMU-HP Students 11.jpeg|thumb|right]]
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<p align="justify">
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The chance to solidify our own knowledge of cloning and synthetic biology in general by teaching it to others was fantastic! We also appreciated the enthusiasm of the students, as they volunteered to participate, and did so on their summer vacation. They had creative and innovative ideas of proteins to fuse to our '''Sporo'''beads. When they left on Monday, the students had learned many new lab techniques, and about the new opportunities that synthetic biology can open up. As a kind gesture of thanks for the practical, the students and their teacher gave us a thank-you card, bottle of champagne, and bag of chocolate bars. Organizing and leading the practical was a great experience.
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The chance to solidify our own knowledge of cloning and synthetic biology in general by teaching it to others was fantastic! As a kind gesture of thanks for the practical, the students and their teacher gave us a thank-you card, bottle of champagne, and bag of chocolate bars.  
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Latest revision as of 13:39, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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