Team:Evry/Notebook/w14

From 2012.igem.org

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<h1>Weeks</h1>
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<h1>Week 14: 10th September - 16th September</h1>
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<p> this week we have worked extensively on a batch of different manipulations including:
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<ul>
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<li>biobricking promotors in Psb1c3 which are:
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            <ul><li>P Elastase</li>
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                    <li>Hsp70</li>
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                    <li>P Car A</li>
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                    <li> FLk1</li>
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                    <li>P CMV</li></ul>
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this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
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<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w2">2</a></td>
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this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles</li>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w3">3</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w4">4</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w5">5</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w6">6</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w7">7</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w8">8</a></td>
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<li>Preparation of chemical Competent Cells</li></ul>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w9">9</a></td>
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</ul>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w10">10</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w11">11</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w12">12</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w13">13</a></td>
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  <td>14</td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w15">15</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w16">16</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w17">17</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w18">18</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w19">19</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w20">20</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w21">21</a></td>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w22">22</a></td>
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</tr>
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</table>
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<h1>Week 14: 10th September - 16th September</h1>
 
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Latest revision as of 00:20, 27 September 2012

Weeks:

June July August September October November

Week 14: 10th September - 16th September

this week we have worked extensively on a batch of different manipulations including:

  • biobricking promotors in Psb1c3 which are:
    • P Elastase
    • Hsp70
    • P Car A
    • FLk1
    • P CMV
    this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
  • Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles
  • Preparation of chemical Competent Cells

Retrieved from "http://2012.igem.org/Team:Evry/Notebook/w14"