Team:Chalmers-Gothenburg

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== Biodetection of human chorionic gonadotropin hormone ==
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The aim of this project was to develop an idea for a biological construct that could be entered in the International Genetically Engineered Machine (iGEM) competition. For this reason, ''Saccharomyces cerevisiae'' have been planned to be modifed in such a way that the yeast cell should function as a biosensor for the human chorionic gonadotropin hormone (hCG). This hormone is produced in the body during pregnancy and consequently, the idea is for the biosensor to function as a simple pregnancy test. To construct the biosensor, the Ste2 receptor in the yeast pheromone signaling pathway will be replaced with the human luteinizing hormone receptor (LH/CG), which is the receptor that hCG binds to with high affnity. The yeast strain that will be used contains a yeast/human chimeric G-alpha subunit, enabling coupling of the receptor with the already existing pheromone pathway in yeast. Consequently, binding of hCG should result in activation of the pathway. The genes tnaA and fmo, encoding tryptophanase and avin-containing monooxygenas  respectively, will also be introduced into the yeast strain. These enzymes catalyze the conversion of tryptophan to indigo. tnaA will be set under the control of the pheromone induced FIG1 promoter and fmo of the constitutive TEF1 promoter. Hence, detection of hCG should result in the production of bio-indigo. In order to ensure hCG to pass the yeast cell wall, the gene CWP2, encoding a cell wall protein, will also be deleted.
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<p><div align="left"  style="margin-left:30px; margin-right:30px; "> <b><font size="7"><b>Welcome</b></font></b>
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to the official wiki of the Chalmers iGEM Team 2012!
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<p><div align="left"  style="margin-left:30px; margin-right:20px; "> <b><font size="5.5">Yeast We Can!</font></b>
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<p><div align="justify"  style="margin-left:30px; margin-right:40px;"> <b><font size="7">Abstract</font></b>
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As our iGEM project, <em>Saccharomyces cerevisiae</em> is planned to be modifed in such a way that the yeast cell should function as a biosensor for the human chorionic gonadotropin hormone (hCG). This hormone is produced in the body during pregnancy and consequently, the idea for the biosensor is to function as a simple pregnancy test. We also plan to introduce genes for bio-indigo production which will be the output signal of the pregnancy test. Below you can see in an illustration how our bio-pregnancy test should function. You can find more details about our project <a href="https://2012.igem.org/Team:Chalmers-Gothenburg/Biodetection_of_hCG">here</a>.
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<p><div align="justify"  style="margin-left:20px; margin-right:20px; "> <b><font size="7"><b>News</b></font></b>
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</br>
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</br><b>26th of Sep:</b> We have been very busy with the summary, analysis and the discussion of our results. Finally, you can find our achievements in the <a href="https://2012.igem.org/Team:Chalmers-Gothenburg/Results">result section</a>. Now we are getting ready for the European Jamboree that takes place next week in Amsterdam! We are very motivated and looking forward to presenting our project for the iGEM jury.</br>See you in Amsterdam!
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</br><b>3rd of Sep:</b> Chalmers iGEM team participated at the <a href="https://2012.igem.org/Team:Chalmers-Gothenburg/Collaboration"> Scandinavian iGEM meet-up </a> weekend in Uppsala. It was very intresting to exchange ideas and experience with other iGEM teams! Thank you Uppsala iGEM Team for a great weekend!
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</br><b>25th of Aug:</b> Our first BioBrick, the yeast FIG1 promoter, is now on its way to the Registry of Standard Biological parts <a href="http://partsregistry.org/Part:BBa_K753000"> BBa_K753000 </a>
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</br>
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<b>17th of Aug:</b> We were holding a presentation for the young students of the <a href="http://sommarforskarskolan.se/"> Astra Zeneca summer science school </a>. A very nice but also challenging alternation to the work in the lab.
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<b>3rd of Aug:</b> We were participating in the UCL iGEM radioshow. Listen to it <a href="https://2012.igem.org/Team:University_College_London/gemFM"> here </a>
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</br>
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<b>23rd of July:</b> We are very happy to present you our new sponsor, <a href="http://www.molnlycke.com/se/">Mölnlycke Health Care</a>! 
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<b>17th of July:</b></br> The sequencing results arrived. All plasmid have perfectly correct inserts without any mutations. Nice!
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</br>
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<b>13th of July:</b> First milestone reached! Our cwp2 deletion is confirmed and the cells seem to be healthy.
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</br>
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<b>5th of July:</b> We can now present a new sponsor of our project: <a href="http://www.gatc-biotech.com/en/index.html">GATC Biotech</a>
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</br>
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<b>20th of June:</b> Today we can be seen on metro.se. Read the article <a href="http://allastudier.se/artiklar/865-labbet-ist%C3%A4llet-f%C3%B6r-stranden-i-sommar">here</a>.
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<b>Old news:</b> Do you want to look up old news? Then you can click <a href="https://2012.igem.org/Team:Chalmers-Gothenburg/Old_news ">here</a>.
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<p><div align="left"  style="margin-left:30px; margin-right:30px; "> <b><font size="7"><b>Links</b></font></b>
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</br>
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Follow us on Facebook and Twitter
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<a href="http://twitter.com/#!/Chalmers_iGEM"><img src="https://static.igem.org/mediawiki/2012/c/ce/Twitt.gif"></a>
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Learn more about iGEM
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Many thanks to our generous sponsors:
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{{Team:Chalmers-Gothenburg/footer}}

Latest revision as of 20:56, 26 September 2012


Welcome to the official wiki of the Chalmers iGEM Team 2012!



Yeast We Can!



Abstract

As our iGEM project, Saccharomyces cerevisiae is planned to be modifed in such a way that the yeast cell should function as a biosensor for the human chorionic gonadotropin hormone (hCG). This hormone is produced in the body during pregnancy and consequently, the idea for the biosensor is to function as a simple pregnancy test. We also plan to introduce genes for bio-indigo production which will be the output signal of the pregnancy test. Below you can see in an illustration how our bio-pregnancy test should function. You can find more details about our project here.

News

26th of Sep: We have been very busy with the summary, analysis and the discussion of our results. Finally, you can find our achievements in the result section. Now we are getting ready for the European Jamboree that takes place next week in Amsterdam! We are very motivated and looking forward to presenting our project for the iGEM jury.
See you in Amsterdam!
3rd of Sep: Chalmers iGEM team participated at the Scandinavian iGEM meet-up weekend in Uppsala. It was very intresting to exchange ideas and experience with other iGEM teams! Thank you Uppsala iGEM Team for a great weekend!
25th of Aug: Our first BioBrick, the yeast FIG1 promoter, is now on its way to the Registry of Standard Biological parts BBa_K753000
17th of Aug: We were holding a presentation for the young students of the Astra Zeneca summer science school . A very nice but also challenging alternation to the work in the lab.
3rd of Aug: We were participating in the UCL iGEM radioshow. Listen to it here
23rd of July: We are very happy to present you our new sponsor, Mölnlycke Health Care!
17th of July:
The sequencing results arrived. All plasmid have perfectly correct inserts without any mutations. Nice!
13th of July: First milestone reached! Our cwp2 deletion is confirmed and the cells seem to be healthy.
5th of July: We can now present a new sponsor of our project: GATC Biotech
20th of June: Today we can be seen on metro.se. Read the article here.
Old news: Do you want to look up old news? Then you can click here.

Links

Follow us on Facebook and Twitter

Learn more about iGEM





















Many thanks to our generous sponsors:

Retrieved from "http://2012.igem.org/Team:Chalmers-Gothenburg"