Team:Queens Canada

From 2012.igem.org

(Difference between revisions)
 
(49 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
<head>
<head>
 +
<link href='http://fonts.googleapis.com/css?family=Signika' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Merienda+One|Philosopher' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Merienda+One|Philosopher' rel='stylesheet' type='text/css'>
<style type="text/css">
<style type="text/css">
 +
body
 +
{
 +
background-image: url('http://dl.dropbox.com/u/46807995/website%20background.png');
 +
font-family: 'Signika', sans-serif;
 +
}
 +
.ca-item-1 .ca-icon{
 +
background-image:url('https://static.igem.org/mediawiki/2012/3/3d/Allflicchimeras.jpeg');
 +
}
 +
.ca-item-2 .ca-icon{
 +
background-image:url('https://static.igem.org/mediawiki/2012/6/61/Chimeric_guide_image.png');
 +
}
 +
.ca-item-3 .ca-icon{
 +
background-image:url('http://dl.dropbox.com/u/46807995/synthetiq.png');
 +
}
 +
.ca-item-5 .ca-icon{
 +
background-image:url('http://dl.dropbox.com/u/46807995/shakinghands.png');
 +
}
 +
.ca-item-6 .ca-icon{
 +
background-image:url('http://dl.dropbox.com/u/46807995/hazard.png');
 +
}
 +
.ca-item-4 .ca-icon{
 +
background-image:url('http://dl.dropbox.com/u/46807995/QGEM%202012%20Logo3.png');
 +
}
 +
 +
.ca-icon
.ca-icon
{
{
Line 39: Line 65:
<div class="ca-content-wrapper">
<div class="ca-content-wrapper">
<div class="ca-content">
<div class="ca-content">
-
<h6>FliCing Bioremediation into action</h6>
+
<h6>Using flagella as scaffolds</h6>
<a href="#" class="ca-close">close</a>
<a href="#" class="ca-close">close</a>
<div class="ca-content-text">
<div class="ca-content-text">
<p>
<p>
-
This year, our team is investigating new methods of increasing the efficiency of bioremediation and biosynthesis using modified bacteria. The development of the oil sands in Alberta, has resulted in the build up of toxic byproducts stored in massive tailings ponds. To help resolve these issues, our goals can be divided up into three main categories: the binding of pollutants, adhesion and aggregation of bacteria, and catalysis.
+
This year, our team is investigating new methods of increasing the efficiency of biosynthesis and bioremediation using modified bacteria. Most bacteria possess tail-like appendages called flagella, which can be genetically altered for novel functions. Each flagellum is made up of a number of polymerizing proteins, often called flagellin.
</p>
</p>
</p>
</p>
-
Most bacteria possess tail-like appendages called flagella, which can be genetically altered for novel functions. Each flagella is made up of a number of polymerizing proteins, often called flagellin. By making chimeric insertions in the variable domain of the flagellin, we can incorporate metal binding proteins, enzymes, adhesive proteins as well as scaffolding proteins to further extend the possible applications.  
+
By making chimeric insertions in the variable domain of the flagellin, we can incorporate fluorescent proteins, enzymes, and scaffolding proteins to extend the possible applications. By having a protein inserted into each monomer, it is possible to cluster thousands of proteins in close proximity to each other, thereby increasing the efficiency of production and break-down of various products.
</p>
</p>
</div>
</div>
<ul>
<ul>
-
<li><a href="#">Read more</a></li>
+
<li><a href="https://2012.igem.org/Team:Queens_Canada/ChimeriQ">Description</a></li>
-
+
<li><a href="https://2012.igem.org/Team:Queens_Canada/ChimeriQ/Results">Results</a></li>
 +
<li><a href="https://2012.igem.org/Team:Queens_Canada/ChimeriQ/Parts">Parts</a></li>
 +
<li><a href="https://2012.igem.org/Team:Queens_Canada/Notebook/Week1">Notebook</a></li>
</ul>
</ul>
</div>
</div>
Line 60: Line 88:
<div class="ca-item-main">
<div class="ca-item-main">
<div class="ca-icon"></div>
<div class="ca-icon"></div>
-
<h3>Team</h3>
+
<h3>Chimeric Proteins</h3>
<h4>
<h4>
<span class="ca-quote">&ldquo;</span>
<span class="ca-quote">&ldquo;</span>
-
<span>There's no I in team</span>
+
<span>A handy guide for introducing you to chimeric protein design.</span>
</h4>
</h4>
<a href="#" class="ca-more">more...</a>
<a href="#" class="ca-more">more...</a>
</div>
</div>
<div class="ca-content-wrapper">
<div class="ca-content-wrapper">
-
<div class="ca-content" >
+
<div class="ca-content">
-
<h6>Team</h6>
+
<h6>How to make chimeric proteins</h6>
<a href="#" class="ca-close">close</a>
<a href="#" class="ca-close">close</a>
-
<div class="ca-content-text" style="padding-bottom: 0px;">
+
<div class="ca-content-text">
-
<img src="http://dl.dropbox.com/u/46807995/teamphoto.jpg" width="600px">
+
<p>There are a lot of considerations that need to be made in the design of chimeric proteins. To help introduce future teams to chimeric proteins and their standard design, we have summarized our work into a resourceful guide that covers all (or almost all) of the considerations that need to be made in designing a chimeric protein from start to finish.</p>
-
<p>QGEM is an undergraduate team composed of both full-time members and volunteers. All faculties of the university are eligible to participate in the iGEM team and previous members have from the departments of  Chemical and Mechanical Engineering, Engineering Chemistry, Biology, Biochemistry, Life Science and Computing.</p>
+
</div>
</div>
<ul>
<ul>
-
<li><a href="https://2012.igem.org/Team:Queens_Canada/Team">Read more</a></li>
+
<li><a href="https://2012.igem.org/Team:Queens_Canada/Guide/DNA">Part 1: DNA </a></li>
 +
<li><a href="https://2012.igem.org/Team:Queens_Canada/Guide/mRNA">Part 2: mRNA</a></li>
 +
<li><a href="https://2012.igem.org/Team:Queens_Canada/Guide/Protein_Structure">Part 3: Protein Structure</a></li>
</ul>
</ul>
</div>
</div>
Line 87: Line 116:
<h4>
<h4>
<span class="ca-quote">&ldquo;</span>
<span class="ca-quote">&ldquo;</span>
-
<span>"insert creative slogan here"</span>
+
<span>The first dance group to perform at a research conference</span>
</h4>
</h4>
<a href="#" class="ca-more">more...</a>
<a href="#" class="ca-more">more...</a>
Line 93: Line 122:
<div class="ca-content-wrapper">
<div class="ca-content-wrapper">
<div class="ca-content">
<div class="ca-content">
-
<h6>SyntheiQ</h6>
+
<h6>SynthetiQ</h6>
<a href="#" class="ca-close">close</a>
<a href="#" class="ca-close">close</a>
<div class="ca-content-text" style="padding-bottom: 0px;">
<div class="ca-content-text" style="padding-bottom: 0px;">
-
<p>SynthetiQ Experimental Dance is a group devoted to creating, testing and analyzing movement, in the form of dance, as a means of explaining scientific concepts. Inspired by Dr. John Bohannon's “Dance Your PhD Contest” and his TEDxBrussels talk in 2011, our first project will be partnered with the Queen's Genetically Engineered Machine (QGEM) Team. Because our research and learning goals align perfectly with those of the QGEM team, we will be researching and presenting together at the International Genetically Engineered Machine Competition in the fall.</p>
+
<p>SynthetiQ Experimental Dance is a group devoted to creating, testing and analyzing movement, in the form of dance, as a means of explaining scientific concepts. Inspired by Dr. John Bohannon's “Dance Your PhD Contest” and his TEDxBrussels talk in 2011, our first project was  partnered with the Queen's Genetically Engineered Machine (QGEM) Team. Because our research and learning goals align perfectly with those of the QGEM team, we will be researching and presenting together at the International Genetically Engineered Machine Competition in the fall.</p>
-
<p>As a part of our 2012 iGEM project, we plan on using dance to explain concepts associated with synthetic biology, as well as our own research.
+
<p>As a part of our 2012 iGEM project, we started this group, devoted to using dance to explain concepts associated with synthetic biology, as well as our own research.
</p>
</p>
<br>
<br>
-
<p>We will make dance into our own unique tool for teaching others scientific concepts, as an alternative to use powerpoint presentations. </p>
+
<p>We made dance into our own unique tool for teaching others scientific concepts, as an alternative to use powerpoint presentations. </p>
</div>
</div>
<ul>
<ul>
Line 110: Line 139:
</div>
</div>
<div class="ca-item ca-item-4">
<div class="ca-item ca-item-4">
 +
<div class="ca-item-main">
 +
<div class="ca-icon"></div>
 +
<h3>Team</h3>
 +
<h4>
 +
<span class="ca-quote">&ldquo;</span>
 +
<span>Who we arespan>
 +
</h4>
 +
<a href="#" class="ca-more">more...</a>
 +
</div>
 +
<div class="ca-content-wrapper">
 +
<div class="ca-content" >
 +
<h6>Team</h6>
 +
<a href="#" class="ca-close">close</a>
 +
<div class="ca-content-text" style="padding-bottom: 0px;">
 +
<img src="http://dl.dropbox.com/u/46807995/teamphoto.jpg" width="600px">
 +
<p>QGEM is an undergraduate team composed of both full-time members and volunteers. All faculties of the university are eligible to participate in the iGEM team and previous members have from the departments of  Chemical and Mechanical Engineering, Engineering Chemistry, Biology, Biochemistry, Life Science and Computing.</p>
 +
</div>
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Queens_Canada/Team">Read more</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="ca-item ca-item-5">
<div class="ca-item-main">
<div class="ca-item-main">
<div class="ca-icon"></div>
<div class="ca-icon"></div>
Line 127: Line 180:
</div>
</div>
<ul>
<ul>
-
<li><a href="#">Read more</a></li>
+
<li><a href="https://2012.igem.org/Team:Queens_Canada/Partners">Read more</a></li>
-
<li><a href="#">Share this</a></li>
+
-
<li><a href="#">Become a member</a></li>
+
-
<li><a href="#">Donate</a></li>
+
</ul>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>
-
<div class="ca-item ca-item-5">
+
<div class="ca-item ca-item-6">
<div class="ca-item-main">
<div class="ca-item-main">
<div class="ca-icon"></div>
<div class="ca-icon"></div>
Line 141: Line 192:
<h4>
<h4>
<span class="ca-quote">&ldquo;</span>
<span class="ca-quote">&ldquo;</span>
-
<span>It's important! More coming soon....</span>
+
<span> Safety doesn't happen by accident.</span>
</h4>
</h4>
<a href="#" class="ca-more">more...</a>
<a href="#" class="ca-more">more...</a>
Line 150: Line 201:
<a href="#" class="ca-close">close</a>
<a href="#" class="ca-close">close</a>
<div class="ca-content-text">
<div class="ca-content-text">
-
<p>More coming soon.....</p>
+
<p> Would any of your project ideas raise safety issues in terms of...researcher safety, public safety, environmental safety?</p>
</div>
</div>
<ul>
<ul>
-
<li><a href="#">Read more</a></li>
+
<li><a href="https://2012.igem.org/Team:Queens_Canada/Safety">Read more</a></li>
-
<li><a href="#">Share this</a></li>
+
 
-
<li><a href="#">Become a member</a></li>
+
-
<li><a href="#">Donate</a></li>
+
</ul>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>
 +
</div>
<!--
<!--
<div class="ca-item ca-item-6">
<div class="ca-item ca-item-6">
Line 250: Line 300:
</script>
</script>
<div id="contentmain">
<div id="contentmain">
-
<i>Our wiki is still under construction! Stay tuned for updates!</i>
+
<iframe align="right" style="width:480px;height:370px;align:right;display:inline;padding:10px;" src="http://www.youtube.com/embed/W3eh2VFe3gs" frameborder="0" style="display:inline" allowfullscreen></iframe>
-
<h3>ChimeriQ</h3>
+
<iframe align="right" style="width:480px;height:370px;align:right;display:inline;padding:10px;" src="http://www.youtube.com/embed/V_eBXVNKvIo" frameborder="0" style="display:inline" allowfullscreen></iframe>
-
<p>
+
 
-
This year, our team is investigating new methods of increasing the efficiency of bioremediation and biosynthesis using modified bacteria. The development of the oil sands in Alberta, has resulted in the build up of toxic byproducts stored in massive tailings ponds. To help resolve these issues, our goals can be divided up into three main categories: the binding of pollutants, adhesion and aggregation of bacteria, and catalysis.
+
-
</p>
+
-
</p>
+
-
Most bacteria possess tail-like appendages called flagella, which can be genetically altered for novel functions. Each flagella is made up of a number of polymerizing proteins, often called flagellin. By making chimeric insertions in the variable domain of the flagellin, we can incorporate metal binding proteins, enzymes, adhesive proteins as well as scaffolding proteins to further extend the possible applications. To accomplish this, we can summarize the majority of our work into three main tasks:  
+
-
</p>
+
-
<ul>
+
-
<li>
+
-
clone and modify the constant domains of the flagellin protein for making insertions using Biobricks and parts obtained from the wild.
+
-
</li>
+
-
<li>
+
-
design a flexible, compatible cloning method for efficientlymaking chimeric insertions using Biobricks and other parts
+
-
</li>
+
-
<li>
+
-
introduce binding and catalysis to the length of the flagella.
+
-
</li>
+
-
</ul>
+
-
<br>Check out our article in <a href="http://www.kingstonthisweek.com/2012/06/21/getting-biological-over-the-break">Kingston This Week!</a>
+
-
</p>
+
-
<h3>SynthetiQ Experimental Dance</h3>
+
-
<p>
+
-
As part of the human practices component of our project, we will be creating a new and unique group devoted to developing dance as a means of teaching, learning, and explaining scientific concepts.
+
-
</p>
+
-
<p>
+
-
Today, scientists and teachers, of any subject, everywhere around the world are working hard to explain their ideas to their students, investors, friends, family and other scientists. Often, we end up in situations where our ideas are so incredible, they are difficult to explain or someone else's explanation of their idea fails to help with your understanding.
+
-
</p>
+
-
<p>
+
-
Inspired by Dr. John Bohannon and the Dance Your PhD Contest, SynthetiQ's primary goal is to provide a new, unique alternative for explaining scientific concepts. By using dance as a means of demonstrating concepts and ideas, we can make complex, or straight-forward, scientific research easier to understand, while leaving a lasting impact on the audience, be it in a classroom, lecture hall, conference or for a panel of investors. An excellent demonstration of this idea can be found in <a href="http://www.youtube.com/watch?v=UlDWRZ7IYqw">Dr. John Bohannon's talk at TedxBrussels</a> from 2011.
+
-
</p>
+
-
<hr/>
+
<h3>
<h3>
How can you reach us?
How can you reach us?

Latest revision as of 03:05, 15 January 2013
Control

ChimeriQ

“ Engineering chimeric flagella for a better world

more...
Using flagella as scaffolds
close

This year, our team is investigating new methods of increasing the efficiency of biosynthesis and bioremediation using modified bacteria. Most bacteria possess tail-like appendages called flagella, which can be genetically altered for novel functions. Each flagellum is made up of a number of polymerizing proteins, often called flagellin.

By making chimeric insertions in the variable domain of the flagellin, we can incorporate fluorescent proteins, enzymes, and scaffolding proteins to extend the possible applications. By having a protein inserted into each monomer, it is possible to cluster thousands of proteins in close proximity to each other, thereby increasing the efficiency of production and break-down of various products.

Chimeric Proteins

“ A handy guide for introducing you to chimeric protein design.

more...
How to make chimeric proteins
close

There are a lot of considerations that need to be made in the design of chimeric proteins. To help introduce future teams to chimeric proteins and their standard design, we have summarized our work into a resourceful guide that covers all (or almost all) of the considerations that need to be made in designing a chimeric protein from start to finish.

SynthetiQ

“ The first dance group to perform at a research conference

more...
SynthetiQ
close

SynthetiQ Experimental Dance is a group devoted to creating, testing and analyzing movement, in the form of dance, as a means of explaining scientific concepts. Inspired by Dr. John Bohannon's “Dance Your PhD Contest” and his TEDxBrussels talk in 2011, our first project was partnered with the Queen's Genetically Engineered Machine (QGEM) Team. Because our research and learning goals align perfectly with those of the QGEM team, we will be researching and presenting together at the International Genetically Engineered Machine Competition in the fall.

As a part of our 2012 iGEM project, we started this group, devoted to using dance to explain concepts associated with synthetic biology, as well as our own research.


We made dance into our own unique tool for teaching others scientific concepts, as an alternative to use powerpoint presentations.

Team

“ Who we arespan>

more...
Team
close

QGEM is an undergraduate team composed of both full-time members and volunteers. All faculties of the university are eligible to participate in the iGEM team and previous members have from the departments of Chemical and Mechanical Engineering, Engineering Chemistry, Biology, Biochemistry, Life Science and Computing.

Partners

“ We would once again like to thank our generous sponsors.

more...
Partners
close

We would like to thank our sponsors who have supported us financially, allowing us to pursue this project. In particular, we would like to thank Queen's University and the Oil Sands Leadership Initiative.

Safety

“ Safety doesn't happen by accident.

more...
Safety
close

Would any of your project ideas raise safety issues in terms of...researcher safety, public safety, environmental safety?

How can you reach us?

info@qgemteam.com

Retrieved from "http://2012.igem.org/Team:Queens_Canada"