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-        padding-top: 60px; /* 60px to make the container go all the way to the bottom of the topbar */
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-      h1 {
-        padding-bottom: 10px;
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-      #footer{
-        padding-top: 60px;
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-    </style>
-    <title></title>
-</head>
-<body id="body-pattern">
-    <div class="container" id="content-container">
-        <header class="jumbotron subhead" id="overview">
-            <h1>Lab Book</p>
-<p></p>
-<h2>Procedure</h2>
-             </header>
-<div class="tabbable">
-  <ul class="nav nav-tabs">
-    <li class="active"><a href="#tab1" data-toggle="tab">General Methods</a></li>
-    <li><a href="#tab2" data-toggle="tab">ω-3 Synthesis</a></li>
-    <li><a href="#tab3" data-toggle="tab">Metal-binding peptides</a></li>
-  </ul>
-  <div class="tab-content">
-    <div class="tab-pane active" id="tab1">
-      <dl class="dl-horizontal">
-        <dt>date</dt>
-        <dd>what we did</dd>
-        <dt>date</dt>
-        <dd>what we did</dd>
-        <dt>date</dt>
-        <dd>what we did</dd>
-       </dl>
-      <p>anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.</p>
-      <p>Please also refer to our <a href="https://2012.igem.org/Team:St_Andrews/Lab-book"><font color="gray">Protocols</font></a> page.</p>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-    </div>
-    <div class="tab-pane" id="tab2">
-<BR>&nbsp;</BR>
-<span class="label"><a href="#Sequences"><font color="white">Sequences</font></a></span>&nbsp;&nbsp;&nbsp;<span class="label"><a href="#Primers"><font color="white">Primers</font></a></span>&nbsp;&nbsp;&nbsp;<span class="label"><a href="#Sequencing"><font color="white">Sequencing results</font></a></span>&nbsp;&nbsp;&nbsp;<span class="label"><a href="#Lipid"><font color="white">Lipid analysis</font></a></span>
-<BR>&nbsp;</BR>
-      <p>Start from lipid extraction</p>
-      <p>primers, sequence results</p>
-<BR>&nbsp;</BR>
-<a name="Sequences"><span class="label">Sequences</span></a>
-<div class="btn-toolbar">
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 T. Cruzi
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-    <div class="span5"><p><font size="1">
-atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtgcttaagtctgggcgcaacaaggagcacaat
-gtcattgtgccttcctccgcactgaccattggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc
-atgtttcttttacgcgacctcgcacaggtggcgttgacgtacgccattatgtacgccgtcgccttgccacttgccacttccatggaagtttctg
-cagcaagaacgttggccgacggtggggtgttatcattgatggcaggtacagccatgacgacagcggcgtggcttttaaaggcagtgttgtg
-ggcggtgttttggtttgtacagggacttaacggcactgcgctttgggtcctggctcacgaatgcggccatcaggcgttcagccccatgaaag
-gtttgaatgacgccgttggcatgattttgcactctgcgctgctcgtgccgtaccacagctggcgcatcacccacggcggccaccacaaac
-acacgaatcacctcacgaaggatcttgtgtttgttccagacaaacgaaacgcggttgtggagctcgtggaggaggcgccgctggtgttatt
-aattcaattattgctgatttttctctttggttggccggcacatcttctttttaatgcctctggacaggaatttggccgactcgcgagccactttgac
-cccggcgctccatttttccgcagcgaagaccgtcacgatattgtcctgtcgaatgttgggattgtcagcgcgttatttgtcattttctccagcgt
-ttaccgctttggttttacaaatgttttctgctggtacattgtaccgtacctctgggtgaacttttggcttgtgtacattacatacctgcagcacacg
-gatatacgcattcctcactacacacatgagcactggacgtttgttcgcggtgcattggcggctgtggacagggactacggctttgtccttaac
-acatggctccatcacatcaatgattcccacgtggtacatcacctctttagcaaaatgccacactacaacgcaatccaggtgacaagaaag
-tacattcgtgagatactgggtgccacatacattacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctatg
-ttgttcccgcagagggcgtctgtgtctttcatgggtaa</font></p></div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 Synechocystis
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-    <div class="span5"><p><font size="1">atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
-attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
-aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat
-ttgggcattatttatctgccctggtactgcttgcccattacctggatctggacagggaca
-gccttaacgggggccttcgttgtcggccatgactgtggccatcgctcctttgctaaaaaa
-cgctgggtcaatgatttagtgggacatatcgcttttgctcccctcatctaccctttccat
-agctggcgcctactccacgaccaccatcacctccacaccaacaaaattgaggttgataac
-gcctgggatccctggagtgtggaagctttccaagccagcccggcgatcgtccggcttttt
-tatcgggccatccggggtcccttctggtggactggttccattttccattggagcttaatg
-cacttcaaactttccaactttgcccaaagggaccgcaataaagtcaaattatccattgcc
-gttgtcttcctgtttgcggcgatcgcctttcctgccctaattatcaccacaggggtgtgg
-ggtttcgtcaaattttggctaatgccctggttggtgtatcacttttggatgagcactttt
-accattgtgcaccacaccattcccgaaattcgtttccgtcccgccgccgattggagtgcc
-gccgaagcccagttaaatggtactgttcactgcgattatccccgttgggtggaagtgctc
-tgccatgacatcaacgtccatattccccaccacctctccgttgccatcccttcctataac
-ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc
-tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
-ggctaccgcaccttcggctccctgaaaaaagtttaa</font></p></div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ15 Synechocystis
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-    <div class="span5"><p><font size="1">gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
-ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
-gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta
-gcggcctaccttgattcctggttcttctatccgattttttggttaattcagggaacccta
-ttctggtccctgtttgtggtgggccatgattgtggccatggctccttttccaaatccaaa
-acccttaataattggattggtcatctcagccacacgccaattttggtgccttaccatggc
-tggcgtattagtcatcgtactcaccatgccaacacgggcaatatcgacaccgacgaaagt
-tggtatccagtgtcggagcaaaaatataaccaaatggcctggtatgaaaaacttctacgt
-ttttacttgcctctgatcgcctaccccatttatctatttcggcgatcgccaaaccggcaa
-ggctcccatttcatgcccggcagtcccctattccgtcccggagaaaaagcagctgttctc
-accagcacctttgcccttgcagcctttgtcggcttccttggctttttaacttggcaattt
-ggctggctatttttgctgaaattttatgttgccccctacctcgtgtttgtggtgtggtta
-gatttggtcacatttttacatcacactgaagacaatatcccttggtatcgtggtgatgac
-tggtattttctcaaaggtgccctctccaccattgatcgggattacggcttcattaacccc
-attcaccatgacattggcacccacgtcgcccaccatattttctcgaatatgccccactac
-aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct
-gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
-caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa</font></p></div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ6 Synechocystis
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-    <div class="span5"><p><font size="1">atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
-caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
-tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt
-gctccagttatttttccggtgcgcctactgggttgtatggttttggcgatcgccttggcg
-gccttttccttcaatgtcggccacgatgccaaccacaatgcctattcctccaatccccac
-atcaaccgggttctgggcatgacctacgattttgtcgggttatctagttttctttggcgc
-tatcgccacaactatttgcaccacacctacaccaatattcttggccatgacgtggaaatc
-catggagatggcgcagtacgtatgagtcctgaacaagaacatgttggtatttatcgtttc
-cagcaattttatatttggggtttatatcttttcattcccttttattggtttctctacgat
-gtctacctagtgcttaataaaggcaaatatcacgaccataaaattcctcctttccagccc
-ctagaattagctagtttgctagggattaagctattatggctcggctacgttttcggctta
-cctctggctctgggcttttccattcctgaagtattaattggtgcttcggtaacctatatg
-acctatggcatcgtggtttgcaccatctttatgctggcccatgtgttggaatcaactgaa
-tttctcacccccgatggtgaatccggtgccattgatgacgagtgggctatttgccaaatt
-cgtaccacggccaattttgccaccaataatcccttttggaactggttttgtggcggttta
-aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg
-gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
-ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga</font></p></div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    ELO 6 L. major
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-     <div class="span5"><p><font size="1">atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
-gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
-aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
-cgcccgaacctcattactctgtcagataaggtgcccctgtcgcagcagggggtgaaggat
-ggctcggtgatcacttacaaggacctcggcccgcagatcggctaccgcacggtgttctac
-gtcgagtatgccggccccatcgccttcatgctgctgtacgccatgcgcccttcgctcatc
-tacggctctgccccgatgccggcttacggctacacgcagaagctatacattggcctcttc
-ctcgcccacttcttaaagcgcgagctcgagtccatgtttgtgcacaagttctcgcaccca
-acgatgccgatgcgcaacatcttcaagaactgcatctactactggtccttcgccgccttc
-atcggctacgtgctgtgcagtccttcattcacgccgaccagcaccatgcagtcaaacttc
-ggcgccgtggtcatggtcatcaacgagctgctgaacttcgcggtgcactaccagcttagc
-acgatgcgcaagtccgatggtgacaccacccgcaacgtgccgcaaggccctctgttcgcc
-ttcgtctcgtgcccgaactacttctttgagattatgtcgtgggtgtccttttccatcggc
-acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg
-gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
-attctgcccttcatcatgtag</font></p></div>
-  </ul>
-</div>
-<BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR>
-<a name="Primers"><span class="label">Primers</span></a>
-<BR>&nbsp;</BR>
-<p>All primers are notated 5' to 3'.
-<BR>&nbsp;</BR>
-<div class="btn-toolbar">
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-   Δ12 T. Cruzi forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-     <div class="span3">ATATCATATGTGGCGGGTTGACAATTTA
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 T. Cruzi reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <p><div class="span3">ATATCTCGAGTTACCCATGAAAGACAC</div></p>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 Synechocystis forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-      <div class="span3">ATATCATATGACTGCCACGATTCCC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 Synechocystis reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-      <div class="span3">ATATCTCGAGTTAAACTTTTTTCAGGGAGC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ15 Synechocystis forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-      <div class="span3">ATATCATATCGCTCTAGAAATTTCATC</div>
-  </ul>
-</div>
-</div>
-<p>
-<div class="btn-toolbar">
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ15 Synechocystis reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-      <div class="span3">ATATCACGACTTAAGGTTTCTTTTGATATC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ6 Synechocystis forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
- <div class="span3">ATATCATATGCAAACAGCGGAAAGAATT</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ6 Synechocystis reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <div class="span4">ATATCTCGAGTAACGATGCTTTGACCATGGCCTCTAG</div>
-  </ul>
-</div></p>
-</div>
-<BR>&nbsp;<?BR>
-<p>When using the pET-duet vector, we needed additionally primers for the alternative restriction sites and His-tags.</p>
-<div class="btn-toolbar">
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    ELO6 L. major forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <div class="span6">TATCATATGGGAAGCAGCCATCATCATCATCATCACAGCATGGTCTCTCTGGAGCAGGCC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ15 T. Cruzi forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <div class="span6">TATCATATGGGCAGCCATCATCATCATCATCACAGCATGCGTCTAGAAATTTCATCG</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ6 T. Cruzi forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <div class="span4">GGATCCGAATTCATCTAACAGCGGAAAGAATT</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ6 T. Cruzi reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-   <div class="span4">GACAAGCTTTCACGATGCTTTGCCCATGGCCTCTAC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 T. Cruzi forward
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-  <div class="span4"> GGATCCGAATTCATGACTGCCACGATTCCC</div>
-  </ul>
-</div>
-<div class="btn-group">
-  <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
-    Δ12 T. Cruzi reverse
-    <span class="caret"></span>
-  </a>
-  <ul class="dropdown-menu">
-<div class="span3">GACAAGCTTTTAAACTTTTTTCAGGGAGC</div>
-  </ul>
-</div>
-</div>
-<BR>&nbsp;</BR>
-<a name="Sequencing"><span class="label">Sequencing results</span></a>
-<BR>&nbsp;</BR>
-<div class="modal hide" id="myModal">
-  <div class="modal-header">
-    <button type="button" class="close" data-dismiss="modal">×</button>
-    <h3>Modal header</h3>
-  </div>
-  <div class="modal-body">
-    <p>One fine body…</p>
-  </div>
-  <div class="modal-footer">
-    <a href="#" class="btn" data-dismiss="modal">Close</a>
-    <a href="#" class="btn btn-primary">Save changes</a>
-  </div>
-</div>
-<dl class="dl-horizontal">
-        <dt>date and gene</dt>
-        <dd>result</dd>
-        <dt>date and gene</dt>
-        <dd>result</dd>
-        <dt>date and gene</dt>
-        <dd>result</dd>
-        <dt>23/7/12 Δ12 Syn.</dt>
-        <dd><p>15b forward gave 0 nucleotides</p>
-            <p>15b reverse</p>
-            <p>20b forward gave a 41 nucleotide result.</p>
-            <div class="btn-group">
-            <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">Sequence<span class="caret"></span>
-            </a>
-               <ul class="dropdown-menu">
-                   <div class="span5"><font size="1">gCGnCctGntGCCgCGCGGCnnnnnnnnnnnnnnnnnngac</font></div>
-               </ul>
-            </div>
-            <p>A BLAST search showed this to be compatible with <i>Crocosphaera watsonii</i> (genome shotgun sequence, 21%), a diazotrophic cyanobacteria. Add in relations to synechocystis.</p>
-            <p>20b reverse</p>
-            <a class="btn" data-toggle="modal" href="#myModal" >Launch Modal</a>
-        </dd>
-      </dl>
-</div>
-<BR>&nbsp;</BR>
-<BR>&nbsp;</BR>
-<a name="Lipid"><span class="label">Lipid analysis</span></a>
-<BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR>
-<BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR><BR>&nbsp;</BR>
-</div>
-<div class="tab-pane" id="tab3">
-      <p>who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely.</p>
-    </div>
-  </div>
-</div>
-</div>
-</body>
-</html>
-{{:Team:St_Andrews/Template:Footer}}

Team:St Andrews/Procedure

From 2012.igem.org

Latest revision as of 11:51, 23 September 2012