Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

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| pSB1A2
| pSB1A2
| 2012 kit plate 1 (13F)
| 2012 kit plate 1 (13F)
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| Incorrect
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| '''Incorrect'''
| Ben
| Ben
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| Razan
| Razan
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| Plasmid Box 1
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| pIGM027
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| Promoter-Theophylline riboswitch 1-Venus-Double terminator
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009]
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| pSB1C3
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| 2012 kit plate 5 (2M)
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| '''Incorrect'''
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| Ben
 +
|-
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| Plasmid Box 1
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| pIGM028
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| Theophylline Riboswitch 1-Venus
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003]
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| pSB1C3
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| 2012 kit plate 5 (7I)
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|
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| Peter/Ben
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| IGM029
| IGM029
| pET-SpA_ScTrp
| pET-SpA_ScTrp
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| Bl-21 D3
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| Bl-21 DE3
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|  
| Logan
| Logan
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| Logan
| Logan
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| iGEM 2012 Box 1
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| IGM031
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| pET-SpA_ScTrp_mCherry_5'
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| Bl-21 DE3
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|
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| Logan
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|-
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| iGEM 2012 Box 1
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|
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| IGM032
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| pET-SpA_ScTrp_mCherry_3'
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| Bl-21 DE3
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|
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| Logan
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|-
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| iGEM 2012 Box 1
 +
|
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| IGM033
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| pET-SpA_ScTrp
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| Bl-21 DE3
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|
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| Logan
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|-
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| iGEM 2012 Box 1
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|
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| IGM034
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| pAAV-miniCMV-mCherry
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| Bl-21 DE3
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|
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| Logan
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|-
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| iGEM 2012 Box 1
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|
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| IGM035
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| psb3k3-J2315.ORF1 + pra301-ndmApro
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| TOP10
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| Barrick Lab
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| Peter
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|-
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| iGEM 2012 Box 1
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|
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| IGM036
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| J23001-NdmABCDgst9-pSB1C3
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| BW25113-guaB
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| Erik
 +
| Erik
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|-
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| iGEM 2012 Box 1
 +
|
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| IGM037
 +
| psb3k3-J2315.ORF1
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| TOP10
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| Barrick Lab
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| Peter
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|-
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| iGEM 2012 Box 1
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|
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| IGM038
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| none
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| BW25113-guaB
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| Erik
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| Erik
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|-
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| iGEM 2012 Box 1
 +
|
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| IGM039
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| pSB1C3-K537011
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| TOP10
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| iGEM parts kit
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| Peter
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|-
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| iGEM 2012 Box 1
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|
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| IGM040
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| pSB1C3-K537003
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| TOP10
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| iGEM parts kit
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| Peter
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|-
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| iGEM 2012 Box 1
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|
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| IGM041
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| NdmABCD
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| BW25113-guaB
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| Erik
 +
| Razan
|}
|}

Latest revision as of 20:22, 11 December 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Label Name Part Plasmid Source Sequence Added by
Plasmid Box 1 cadA ASKA cadA plasmid Barrick Lab
Plasmid Box 1 ldcC ASKA ldcC plasmid Barrick Lab
Plasmid Box 1 pIGM001 LasR Promoter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] pSB1A2 2012 kit plate 1 (12A) Verified Razan
Plasmid Box 1 pIGM002 LasR [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] pSB3K3 2012 kit plate 1 (14J) Verified Razan
Plasmid Box 1 pIGM003 Lac Promoter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] pSB1A2 2012 kit plate 1 (1D) Razan
Plasmid Box 1 pIGM004 GFP [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L) Verified Ben
Plasmid Box 1 pIGM005 RFP [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O) Verified Ben
Plasmid Box 1 pIGM006 LasI [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N) Verified Ben
Plasmid Box 1 pIGM007 RBS + LacI [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] pSB1A2 2012 kit plate 1 (7L) Verified Aurko
Plasmid Box 1 pIGM008 Double terminator (BBa_B0010 - BBa_B0012) [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] pSB1AK3 2012 kit plate 1 (23L) Verified Aurko
Plasmid Box 1 pIGM009 J23100 promoter - sfGFP (Part:BBa_K515105) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] pSB1C3 2012 kit plate 5 (1K)
Plasmid Box 1 pIGM014 Plasmid pSB1A3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1A3 2012 kit plate 1 (1G) Logan
Plasmid Box 1 pIGM015 Plasmid pSB1C3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1C3 2012 kit plate 1 (3A) Logan
Plasmid Box 1 pIGM016 Plasmid pSB1K3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1K3 2012 kit plate 1 (5A) Logan
Plasmid Box 1 pIGM017 Plasmid pSB1T3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1T3 2012 kit plate 1 (7A) Logan
Plasmid Box 1 pIGM021 RFP [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] pSB1A2 2012 kit plate 1 (13F) Incorrect Ben
Plasmid Box 1 pIGM022 pAK.TAQ Jared Mike
Plasmid Box 1 pIGM023 pET21.KOD Jared Mike
Plasmid Box 1 pIGM024 pQL269 Jared Mike
Plasmid Box 1 pIGM025 pACYC.T7.GFP Jared Mike
Plasmid Box 1 pIGM026 sfGFP pET21 Razan
Plasmid Box 1 pIGM027 Promoter-Theophylline riboswitch 1-Venus-Double terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009] pSB1C3 2012 kit plate 5 (2M) Incorrect Ben
Plasmid Box 1 pIGM028 Theophylline Riboswitch 1-Venus [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003] pSB1C3 2012 kit plate 5 (7I) Peter/Ben

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Label Description Strain Source Added by
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 IGM004 pSB3K3-I714891 (GFP - but not used in construct) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM005 pSB1AK3-K081014 (RFP) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM006 pSB1A2-K081009 (LasI) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM009 crdASC (4864 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM010 crdASC (4943 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM011 crdASC (4864 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 IGM012 crdASC (4943 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 11 IGM013 BBa_K515105: J23100-sfGFP-pSB1C3(CamR) epi300 Parts Kit
iGEM 2012 Box 1 IGM014 pSB1A3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM015 pSB1C3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM016 pSB1K3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM017 pSB1T3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM018 pSB1A2 with LasR Promoter Bba_R0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM019 pSB2K3 with LasR Bba_C0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM020 pSB1A2 with Lac Promoter Bba_R0010 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM021 J23151_GFP_pSB3K3: Bba_I20270 TOP10 Parts Kit Erik
iGEM 2012 Box 1 IGM022 J23101_GFP_pSB3K3: Bba_I20260 TOP10 Parts Kit Erik
iGEM 2012 Box 1 IGM023 pet21.KOD TOP10 Jared
iGEM 2012 Box 1 IGM024 pQL269 TOP10 Jared
iGEM 2012 Box 1 IGM025 pACYC.T7.GFP TOP10 Jared
iGEM 2012 Box 1 IGM026 pSB1A2-J06504 (RFP--incorrect sequence) TOP10 Parts Kit Ben
iGEM 2012 Box 1 IGM027 pAK.TAQ TOP10 Jared
iGEM 2012 Box 1 IGM028 PAAV-miniCMV-mCherry Bl-21 AI Addgene Logan
iGEM 2012 Box 1 IGM029 pET-SpA_ScTrp Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM030 pET-SpA_ScTrp_mCherry_5' Bl-21 AI Logan
iGEM 2012 Box 1 IGM031 pET-SpA_ScTrp_mCherry_5' Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM032 pET-SpA_ScTrp_mCherry_3' Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM033 pET-SpA_ScTrp Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM034 pAAV-miniCMV-mCherry Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM035 psb3k3-J2315.ORF1 + pra301-ndmApro TOP10 Barrick Lab Peter
iGEM 2012 Box 1 IGM036 J23001-NdmABCDgst9-pSB1C3 BW25113-guaB Erik Erik
iGEM 2012 Box 1 IGM037 psb3k3-J2315.ORF1 TOP10 Barrick Lab Peter
iGEM 2012 Box 1 IGM038 none BW25113-guaB Erik Erik
iGEM 2012 Box 1 IGM039 pSB1C3-K537011 TOP10 iGEM parts kit Peter
iGEM 2012 Box 1 IGM040 pSB1C3-K537003 TOP10 iGEM parts kit Peter
iGEM 2012 Box 1 IGM041 NdmABCD BW25113-guaB Erik Razan
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"