J/19 July 2012

From 2012.igem.org

(Difference between revisions)

Latest revision as of 10:18, 23 July 2012

July
M	T	W	T	F	S	S
						[http://2012.igem.org/J/1_July_2012 1]
[http://2012.igem.org/J/2_July_2012 2]	[http://2012.igem.org/J/3_July_2012 3]	[http://2012.igem.org/J/4_July_2012 4]	[http://2012.igem.org/J/5_July_2012 5]	[http://2012.igem.org/J/6_July_2012 6]	[http://2012.igem.org/J/7_July_2012 7]	[http://2012.igem.org/J/8_July_2012 8]
[http://2012.igem.org/J/9_July_2012 9]	[http://2012.igem.org/J/10_July_2012 10]	[http://2012.igem.org/J/11_July_2012 11]	[http://2012.igem.org/J/12_July_2012 12]	[http://2012.igem.org/J/13_July_2012 13]	[http://2012.igem.org/J/14_July_2012 14]	[http://2012.igem.org/J/15_July_2012 15]
[http://2012.igem.org/J/16_July_2012 16]	[http://2012.igem.org/J/17_July_2012 17]	[http://2012.igem.org/J/18_July_2012 18]	[http://2012.igem.org/J/19_July_2012 19]	[http://2012.igem.org/J/20_July_2012 20]	[http://2012.igem.org/J/21_July_2012 21]	[http://2012.igem.org/J/22_July_2012 22]
[http://2012.igem.org/J/23_July_2012 23]	[http://2012.igem.org/J/24_July_2012 24]	[http://2012.igem.org/J/25_July_2012 25]	[http://2012.igem.org/J/26_July_2012 26]	[http://2012.igem.org/J/27_July_2012 27]	[http://2012.igem.org/J/28_July_2012 28]	[http://2012.igem.org/J/29_July_2012 29]
[http://2012.igem.org/J/30_July_2012 30]	[http://2012.igem.org/J/31_July_2012 31]

Day Nineteen

(10:00 am) Next the group tried out shaving EPS into acetone to see if the cloudy dissolved solution of acetone, eps and distilled water could be recreated. The solution turned cloudy but not as much as before, so it is being placed and left in the fume cupbaord to see what happens.

(10:30 am) A few members of the group also tried plating out the 10g M.A. plate then sprinkling polystyrene "sugar" onto the surface rather than mixing it into the agar. This produced good results as the sugar made a layer just under the surface of the agar rather than sinking to the bottom, making it easier and more cost effective as less of the resources are needed.

Step one: measure out 0.5g of the polystyrene "sugar" on a zeroed balance using a measuering paper receptical.

Step two: make sure the agar is molten, and your petridish is slightly warmed in the hybridiser so the small amount of agar used does not set strait away.

Step three: place the empty pre labled petridish onto the balance and zero. After this poor 9.50g of the heated M.A Agar into the petrisish and remove from the balance to a level table.

Setp four: sprinkle the polystyrene "sugar" onto the agar making sure there is an even spread, replace the lid and allow to cool. Once set turn the plate over so condensation doesn't drip onto the agar.

We noticed after the sprinking, then agar set quickly, resulting in a thin layer of the polystyrene at the surface of the dish, which was the aim of the process.

(11:00 am) Another step the group wanted to try was to gently heat the polystryene "sugar" to see if it can be formed into more of a liquid to work with, as this could be easily spread into a thin layer.

(15:00 pm) After the group had lunch and finished up some work in the lab, we went to the Pseudomonas lab to see what had happened with the plates, and if it had grown or not. Unfortunately it had grown on the minimal media without polystyrene purely on the tiny amount of agar to make the gel set. With this new complication, the group headed back to the lab to decide on the next course of action, before stopping for the day.

@@ Line 11: / Line 11: @@
 {|align="justify"
-|===== Day Nine =====
+|
-(10:00 am) chris tried out shaving EPS into acetone to see if we could recreate the cloudy disolved solution of acetone, eps and distilled water, the solution turned cloudy but not as much as before.. so is not in the fume cupbaord to see what happens.
+===== Day Nineteen =====
-also have tried plating out the 10g M.A. plate then sprinkling "sugar" polystyrene onto the surface rather than mixing it into the agar, so far this has produced good results as the sugar has remained just under the surface of the agar rather than sinking to the bottom, making it easier and more cost effective as less glass wear is needed.
+(10:00 am) Next the group tried out shaving EPS into acetone to see if the cloudy dissolved solution of acetone, eps and distilled water could be recreated. The solution turned cloudy but not as much as before, so it is being placed and left in the fume cupbaord to see what happens.
-Step one: measuer out 0.50g of the sugar polystyrene on a 0.00 balance using a measuering paper receptical, making sure to zero after adding the recepical before pooring the polystyrene.
-Step two: make sure your agar has been melted, and your petridish is slightly warmed in the hybridiser so the small amount of agar we are using does not set strait away.
-Step three: place the empty pre labled petridish onto the blanace and zero. After this poor 9.50g of the heated M.A Agar into the petrisish and remove from the balance to a level table,
-Setp four: sprinkle the sugar polystyrene onto the agar making sure you have a even spread, replace the lid and allow to cool.
-We noticed after the sprinking, then agar set quickly, resulting in a thin layer of the polystyrene at the surface of the dish, which is what we were trying to achieve.
+(10:30 am) A few members of the group also tried plating out the 10g M.A. plate then sprinkling polystyrene "sugar" onto the surface rather than mixing it into the agar. This produced good results as the sugar made a layer just under the surface of the agar rather than sinking to the bottom, making it easier and more cost effective as less of the resources are needed.
-(11:00am) Chris and will are now going to try and gentally heat polystryene sugar to see if we can form it into more of a liquid to then work with, as this could be easily spread into a thin layer.
+Step one: measure out 0.5g of the polystyrene "sugar" on a zeroed balance using a measuering paper receptical.
+Step two: make sure the agar is molten, and your petridish is slightly warmed in the hybridiser so the small amount of agar used does not set strait away.
+Step three: place the empty pre labled petridish onto the balance and zero. After this poor 9.50g of the heated M.A Agar into the petrisish and remove from the balance to a level table.
+Setp four: sprinkle the polystyrene "sugar" onto the agar making sure there is an even spread, replace the lid and allow to cool. Once set turn the plate over so condensation doesn't drip onto the agar.
+We noticed after the sprinking, then agar set quickly, resulting in a thin layer of the polystyrene at the surface of the dish, which was the aim of the process.
+(11:00 am) Another step the group wanted to try was to gently heat the polystryene "sugar" to see if it can be formed into more of a liquid to work with, as this could be easily spread into a thin layer.
+(15:00 pm) After the group had lunch and finished up some work in the lab, we went to the ''Pseudomonas'' lab to see what had happened with the plates, and if it had grown or not. Unfortunately it had grown on the minimal media without polystyrene purely on the tiny amount of agar to make the gel set. With this new complication, the group headed back to the lab to decide on the next course of action, before stopping for the day.