Team:WashU/Week8
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- | The gel of these digests is found below. | + | The gel of these digests is found below. |
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<div align = "center"><img src="https://lh3.googleusercontent.com/-htrirqNSahQ/UAbRBZ6vPfI/AAAAAAAAAVU/fTlb30i-V-I/s800/digestalotostuff-2.jpg" width=350/></div> <br> | <div align = "center"><img src="https://lh3.googleusercontent.com/-htrirqNSahQ/UAbRBZ6vPfI/AAAAAAAAAVU/fTlb30i-V-I/s800/digestalotostuff-2.jpg" width=350/></div> <br> | ||
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We have started cultures of <i>E. coli</i> doubly transformed with our Z construct and CS42S at different chloramphenicol concentrations. We will miniprep them after they grow to obtain the DNA. | We have started cultures of <i>E. coli</i> doubly transformed with our Z construct and CS42S at different chloramphenicol concentrations. We will miniprep them after they grow to obtain the DNA. | ||
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We repeated the digests and PCRs from yesterday in order to ascertain what went wrong. The PCRs yielded primer dimers and were thus unsuccessful, so we plan on troubleshooting our procedure to determine why our reactions are failing. | We repeated the digests and PCRs from yesterday in order to ascertain what went wrong. The PCRs yielded primer dimers and were thus unsuccessful, so we plan on troubleshooting our procedure to determine why our reactions are failing. | ||
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In order to increase our output of carotenoids in <i>Synechocystis</i>, we began a PAL mutant liquid culture of the cyanobacterium in order to transform with in the next few days. | In order to increase our output of carotenoids in <i>Synechocystis</i>, we began a PAL mutant liquid culture of the cyanobacterium in order to transform with in the next few days. | ||
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+ | After using a PCR cleanup kit to purify our results from yesterday, we then digested the constructs and ligated them into plasmids, and plated the final products. | ||
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- | <td2><a href="/Team:WashU/ | + | <td2><a href="/Team:WashU/Week7"><img src ="http://aux4.iconpedia.net/uploads/937908040.png" width=82px height=82px></a></td2> |
- | <td2><a href="/Team:WashU/ | + | <td2><a href="/Team:WashU/Week9"><img src ="http://aux.iconpedia.net/uploads/1782679686.png" width=82px height=82px></a></td2> |
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Latest revision as of 02:18, 15 September 2012