Team:Penn

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''Project Description:
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This year our project plans to tackle the problems of health and medicine through the lens of synthetic biology.
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Part 1: Light-activated Drug Delivery
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                <ul id="rotmenu">
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<li>
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                        <a href="rot1" style="font-size:12px;"><b>Regional Winners!</b></a>
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                        <div style="display:none;">
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                            <div class="info_image">2/2c/Regional-Winners-Slider.jpg</div>
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                            <div class="info_heading"></div>
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                                Penn iGEM placed first in the Americas East Regional Jamboree and advanced to the World Championships!
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                                <a href="https://igem.org/Results?year=2012&division=igem&region=Americas_East" class="more">Read more</a>
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                            </div>
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                        </div>
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                        <a href="rot2" style="font-size:12px;"><p><b>Light Induced</b></p><p><b>Lysis</b></p></a>
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                        <div style="display:none;">
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                            <div class="info_image">4/4f/Light-Induced-Lysis-Slider.jpg</div>
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                            <div class="info_heading">pDAWN ClYA construct</div>
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<div class="info_description">We have developed light-activated cell lysis using the YF1/FixJ Blue Light sensor and the ClyA protein.
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<br>
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<a href="https://2012.igem.org/Team:Penn/DrugDeliveryResults" class="more">Read more</a>
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                            </div>
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                        </div>
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                        <a href="rot3" style="font-size:12px;"><p><b>Novel Surface</b></p><p><b>Display</b></p></a>
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                            <div class="info_image">4/46/Novel-Surface-Display-Slider.jpg</div>
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                            <div class="info_heading">Display of ANTi-her2 DARPin</div>
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                            <div class="info_description">
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Our team is the first to use the INPNC protein to display the DARPin Anti-HER2 binding protein on the surface of bacteria <br>
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                                <a href="https://2012.igem.org/Team:Penn/DrugDeliveryResults" class="more">Read more</a>
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                            </div>
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                        </div>
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                    </li>
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                        <a href="rot4" style="font-size:12px;"><b>Outreach</b></a>
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                        <div style="display:none;">
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                            <div class="info_image">5/53/Outreach-Slider-Reverse.jpg</div>
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                            <div class="info_heading">Clark Park Science Fair</div>
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                            <div class="info_description">
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Learn more about our education outreach with high schoolers and West Philadelphia residents!
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<br>
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                                <a href="https://2012.igem.org/Team:Penn/Outreach" class="more">Read more</a>
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                            </div>
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                        </div>
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                    </li>
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                    <li>
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                        <a href="rot5" style="font-size:12px;"><b>Lab Work</b></a>
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                        <div style="display:none;">
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                            <div class="info_image">3/36/Lab-Work-Slider2.jpg</div>
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                            <div class="info_heading"></div>
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                            <div class="info_description">
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Learn about cool experiments the team performed this summer!
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<br>
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                                <a href="https://2012.igem.org/Team:Penn/Notebook" class="more">Read more</a>
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                            </div>
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                        </div>
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One of the most pervading diseases afflicting the lives of countless individuals is cancer. Current widespread therapies such as chemotherapy and radiation therapy are limited in their ability to serve as a highly successful cancer therapeutic due to their off-target effects. As a result, one of our projects aims to solve some of these problems of specificity through an effective synthetic system that stems from the field of optogenetics. By using a system that is controlled by light we will engineer bacteria so that it kills cancerous cells with increased specificity in three dimensions. First, the bacteria will be able to specifically target cancerous regions through the use of antibody mimetic proteins (DARPins) that bind to growth factors which are commonly found on the surface of cancer cells. Second, because the system is light-activated we will be able to increase specificity by temporally controlling the duration of the cancer treatment. Since we have the ability to determine the exposure of light to the region of the tumor, the times between doses can be easily controlled. Third, since this treatment strategy has the additional advantage of being spatially-controlled by light, the drug does not lyse normal, unaffected cells.
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                    <div class="description">
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                        <p></p>
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Part 2: Engineering microbial biofilms
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Bacteria are capable of surviving on many surfaces, where they often organize into structured and highly resilient communities known as “biofilms.” Recent research has discovered that many pathogenic bacteria are capable of forming biofilms that are difficult to eradicate and even more difficult to treat. Many chemical approaches have been applied to preventing biofilm formation, especially in invasive devices such as catheters, as well as in the food processing industry. However, these devices carry the drawbacks of loss of function over time (as a result of the depletion of antimicrobial elements from the surface) and also safety concerns when used in vivo.
 
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This project seeks to investigate a biological approach rather than a chemical approach to antimicrobial surfaces. Surfaces will be colonized with a non-pathogenic, biofilm forming bacterium that would secrete antimicrobial peptides (AMP) that would inhibit subsequent colonization of the surface by pathogenic microbes. In our project, we have chosen an AMP known as lysostaphin, which selectively targets Staphylococcus bacteria, a biofilm forming genus that is a primary culprit of hospital acquired infections. Furthermore, the bacteria that form our biofilm will be programed with a bacterial “leash,” that prevents them from surviving should they become detached from the biofilm. This approach has never been attempted before in the healthcare and food production fields. Should our system function properly, it would set the groundwork for potential commercial technologies that would lead to healthier lives, and safer foods.''
 
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|[[Image:Penn_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:Penn | Team Penn]]
 
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!align="center"|[[Team:Penn|Home]]
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<td style="width:250px;">
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!align="center"|[[Team:Penn/Team|Team]]
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<table class="box" style="margin-top:20px;height:305px;width:220px;margin-left:10px;margin-right:10px;">
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Penn Official Team Profile]
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!align="center"|[[Team:Penn/Project|Project]]
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<td>
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!align="center"|[[Team:Penn/Parts|Parts Submitted to the Registry]]
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    <div style="font-size:20px;height:200px;width:220px;">
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!align="center"|[[Team:Penn/Modeling|Modeling]]
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        <div>
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!align="center"|[[Team:Penn/Notebook|Notebook]]
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        <h2><b>Welcome to the Penn Wiki!</b></h2>
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!align="center"|[[Team:Penn/Safety|Safety]]
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!align="center"|[[Team:Penn/Attributions|Attributions]]
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<p style="text-align:center; font-size: 12px; font-weight:bold; font-color: white; padding-left:5px; padding-right:5px;">
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With the help of our sponsors, the Penn iGEM Team is excited to compete for its second year! </p>
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<p style="text-align:center; font-size: 12px; font-weight:bold; font-color: white; padding-left:5px; padding-right:5px;">
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We have been working hard all summer and are proud of what we have accomplished.
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</p>
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<p style="text-align:center; font-size: 12px; font-weight:bold; font-color: white; padding-left:5px; padding-right:5px;">
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Please, take a look around!
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</p>
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<p style="text-align:center; font-size: 14px; font-weight:bold; font-color: white;"><u>Quick Links</u></p>
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<p style="text-align:center; font-size: 14px; font-weight:bold; font-color: white;"><a href="https://2012.igem.org/Team:Penn/ProjectResults" class="white">Project Overview</a></p>
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<p style="text-align:center; font-size: 14px; font-weight:bold; font-color: white;"><a href="https://2012.igem.org/Team:Penn/Parts" class="white">Parts Submitted</a></p>
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<p style="text-align:center; font-size: 14px; font-weight:bold; font-color: white;"><a href="https://2012.igem.org/Team:Penn/Team" class="white">About the Team</a></p>
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Latest revision as of 19:22, 24 August 2013

Penn 2012 iGEM Wiki

Image Map
  • Regional Winners!
    2/2c/Regional-Winners-Slider.jpg
    Penn iGEM placed first in the Americas East Regional Jamboree and advanced to the World Championships! Read more

  • Light Induced

    Lysis
    4/4f/Light-Induced-Lysis-Slider.jpg
    pDAWN ClYA construct
    We have developed light-activated cell lysis using the YF1/FixJ Blue Light sensor and the ClyA protein.
    Read more

  • Novel Surface

    Display
    4/46/Novel-Surface-Display-Slider.jpg
    Display of ANTi-her2 DARPin
    Our team is the first to use the INPNC protein to display the DARPin Anti-HER2 binding protein on the surface of bacteria
    Read more
  • Outreach
    5/53/Outreach-Slider-Reverse.jpg
    Clark Park Science Fair
    Learn more about our education outreach with high schoolers and West Philadelphia residents!
    Read more
  • Lab Work
    3/36/Lab-Work-Slider2.jpg
    Learn about cool experiments the team performed this summer!
    Read more

Welcome to the Penn Wiki!

With the help of our sponsors, the Penn iGEM Team is excited to compete for its second year!

We have been working hard all summer and are proud of what we have accomplished.

Please, take a look around!

Quick Links

Project Overview

Parts Submitted

About the Team


Retrieved from "http://2012.igem.org/Team:Penn"