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| - | {{Team:HokkaidoU_Japan/header}}
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| - | {{Team:HokkaidoU_Japan/nav.notebook}}
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| - | <div id="hokkaidou-column-main">
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| - | <!-- DO NOT EDIT OVER THIS LINE @iTakeshi -->
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| - | ==July 16th==
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| - | <div class="hokkaidou-notebook-daily">
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| - | ===Digestion===
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| - | Results of digestion in 15th.
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| - | [[image:HokkaidoU2012 120716 Ag43 on pSB1C3 digestion with S&P-1.jpg|thumb|Digestion result image]]
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| - | Product:Ag43(K346007)=3120bp and 2070bp, pT7-RBS on pSB1K3=2247bp
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| - | We confirmed there are some kind of restriction enzyme site in K346007 (digested with SpeI, PstI) and pT7-RBS on pSB1K3 was successfully digested with EcoRI and PstI. Balance between d+(EcoRI) and d+(E&P:cut with EcoRI and PstI) is about 80bp.
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| - | ===Gel Extraction===
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| - | Gel Extraction for digestion products. We used FastGene Gel&PCR extraction kit(NipponGenetics).
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| - | Got 50ul of DNA solution.
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| - | ===Ethanol Precipitation===
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| - | Ethanol Precipitation for digestion and gel extraction products.
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| - | #Added 5ul of NaoAc, 1.5ul of glycogen and 125ul of 100% ethanol.
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| - | #Centrifuged in 15000rpm, 10min at 4C.
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| - | #Remove supernatant and added 220ul of 70% ethanol.
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| - | #Centrifuged in 15000rpm, 10min at 4C.
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| - | #Remove supernatant and air drying in room temperature then added 5ul of DW.
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| - | <!-- DO NOT EDIT UNDER THIS LINE @iTakeshi -->
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| - | </div>
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| - | <br style="line-height: 0; clear: both;" />
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| - | {{Team:HokkaidoU_Japan/footer}}
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