Team:Peking

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<h2>Programming Cells to Talk Through Light</h2>
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<p style="text-align:justify;">During the last few decades, chemically regulated genetic systems have been thoroughly developed and analyzed. Though remarkable endeavors were made towards this issue, the disadvantages of chemical regulation remain: the high cost of chemical synthesis, the diffusion limits, the insecurity and the limited choices of chemicals. </p>
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<p style="text-align:justify;">Such incommodity calls for a new generation of optogenetics expression systems. Since first demonstrated in 2002, the contributions of optogenetics have been far beyond that of neuroscience due to the fact that light is more controllable, compared with chemicals, to regulate molecular and cellular behavior. However, most optogenetics methods rely on laser, which limits their field application. This summer, Peking iGEM is endeavoring on developing a new luminescence sensor, which is expected to be sensitive to natural light and even bio-luminescence. Based on this ultrasensitive luminescence sensor, we are trying to program cells to talk through light. The light-communication between cells attempts to overcome various limitations of conventional quorum sensing and accomplish ‘genuine’ self-organization within a population beyond chemical diffusion. </p>
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Sowing Tomorrow's Synthetic Biologists
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  <div style="float:left;"><p style="font-size:45px;line-height:45px;color:#ffffff;margin:20px 3px 0 20px;">L</p></div>
 +
  <p class="context_block" style="width:610px;">
 +
ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the <b style="color:#ffee55;"> <i>Luminesensor</i></b>. Amazingly, the <i>Luminesensor</i> was proven to be so sensitive as to be able to detect <b style="color:#ffee55;">natural light</b> and even <b style="color:#ffee55;">bioluminescence</b>. With this sensor, spatiotemporal control of cellular behavior, such as <b style="color:#ffee55;">high-resolution 2D</b> and <b style="color:#ffee55;">3D bio-printing</b> using dim light and even the <b style="color:#ffee55;">luminescence of iPad</b> were shown to be very easy. What’s more, we successfully implemented <b style="color:#ffee55;">cell-cell signaling using light</b> for the very first time in synthetic biology, which will be of great importance for biotechnological use.
 +
  </p>
 +
</div>
 +
<div class="floatL" style="margin:0 10px 5px 0;"><table class="title_block" style="background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><tr><td style="height:244px;"><p style="font-size:36px;">Abstract</p></td></tr></table></div>
 +
 
 +
<div class="floatC"><p style="height:30px;"></p></div>
 +
<div class="floatL">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/a/a4/Peking2012_Color_Title_FlameYellow.jpg');"><tr><td><a href="/Team:Peking/Project/Luminesensor" style="font-size:26px;">Luminesensor</a></tr></td></table></div>
 +
  <div class="floatR"><img src="/wiki/images/4/4f/Peking2012_LuminesensorMechanism.jpg" alt="" style="width:200px;"/></div>
 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us <i>Luminesensor</i>, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.
 +
  </p></div>
 +
</div>
 +
<div class="floatR">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/a/a2/Peking2012_Color_Title_FreshGreen.jpg');"><tr><td><a href="/Team:Peking/Project/Communication" style="font-size:26px;">Light <br /> Communication</a></tr></td></table></div>
 +
  <div class="floatR"><img src="/wiki/images/7/77/Peking2012_LightCommunication.jpg" alt="" style="width:200px;"/></div>
 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
The <i>Luminesensor</i> is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.
 +
  </p></div>
 +
</div>
 +
<div class="floatC"><p style="height:30px;"></p></div>
 +
<div class="floatL">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/f/fe/Peking2012_Color_Title_GrapePurple.jpg');"><tr><td><a href="/Team:Peking/Project/3D">Syn Bio in <br /> 2D & 3D</a></tr></td></table></div>
 +
  <div class="floatR"><img src="/wiki/images/b/b1/Peking2012_PrintParagraph_Square_Edit.jpg" alt="" style="width:200px;"/></div>
 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
3D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our <i>Luminesensor</i> to implement 3D printing that can be utilized in many medical or manufacturing applications.
 +
  </p></div>
 +
</div>
 +
<div class="floatR">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/5/50/Peking2012_Color_Title_CherryPink.jpg');"><tr><td><a href="/Team:Peking/Project/Phototaxis">Phototaxis</a></tr></td></table></div>
 +
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 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
"Phototatic" bacteria can be built by programming the chemotaxis system in <i>E. coli</i> through light. By controlling the expression level of the CheZ protein with the <i>Luminesensor</i>, the tumbling frequency is coupled to the intensity of light signals.
 +
  </p></div>
 +
</div>
 +
<div class="floatC"><p style="height:30px;"></p></div>
 +
<div class="floatL">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/a/a2/Peking2012_Color_Title_FreshGreen.jpg');"><tr><td><a href="/Team:Peking/HumanPractice/Sowing">Human <br /> Practice</a></tr></td></table></div>
 +
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 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.
 +
  </p></div>
 +
</div>
 +
<div class="floatR">
 +
  <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><tr><td><a href="/Team:Peking/Modeling">Modeling</a></tr></td></table></div>
 +
  <div class="floatR"><img src="/wiki/images/a/a8/Peking2012_PhototaxisSPECSdot_Edit.gif" alt="" style="width:200px;"/></div>
 +
  <div class="floatC"><p class="context_block" style="width:360px;">
 +
Excellent stochastic simulation was conducted to describe our ultrasensitive <i>Luminesensor</i>, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.   
 +
  </p></div>
 +
</div>
 +
<div class="floatC"><p style="height:80px;"></p></div>
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<div id="sponsor" class="floatC">
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    <a href="http://cqb.pku.edu.cn"><img src="/wiki/images/f/f8/Peking2012_Sponsor_CQB.jpg" alt="" style="width:530px;"/></a>
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 +
    <a href="http://dean.pku.edu.cn"><img src="/wiki/images/b/be/Peking2012_Sponsor_PkuEduAdministration.jpg" alt="" style="width:170px;"/></a>
 +
  </td><td>
 +
    <a href="http://www.genscript.com"><img src="/wiki/images/9/9b/Peking2012_Sponsor_GenScript.jpg" alt="" style="width:152px;"/></a>
 +
  </td><td>
 +
    <a href="http://www.scitech-youth.org.cn"><img src="/wiki/images/3/36/Peking2012_Sponsor_BeijingTeenagerST.jpg" alt="" style="width:170px;"/></a>
 +
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<p class="blank" style="width:0;height:134px;">&#32;</p>
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Latest revision as of 20:16, 26 October 2012

L

ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the Luminesensor. Amazingly, the Luminesensor was proven to be so sensitive as to be able to detect natural light and even bioluminescence. With this sensor, spatiotemporal control of cellular behavior, such as high-resolution 2D and 3D bio-printing using dim light and even the luminescence of iPad were shown to be very easy. What’s more, we successfully implemented cell-cell signaling using light for the very first time in synthetic biology, which will be of great importance for biotechnological use.

Abstract

Luminesensor

Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us Luminesensor, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.

Light
Communication

The Luminesensor is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.

Syn Bio in
2D & 3D

3D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our Luminesensor to implement 3D printing that can be utilized in many medical or manufacturing applications.

Phototaxis

"Phototatic" bacteria can be built by programming the chemotaxis system in E. coli through light. By controlling the expression level of the CheZ protein with the Luminesensor, the tumbling frequency is coupled to the intensity of light signals.

Human
Practice

We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.

Modeling

Excellent stochastic simulation was conducted to describe our ultrasensitive Luminesensor, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.

Retrieved from "http://2012.igem.org/Team:Peking"