Team:Trieste

From 2012.igem.org

(Difference between revisions)
(added title)
(fix layout)
 
(103 intermediate revisions not shown)
Line 1: Line 1:
 +
           
 +
{{Team:Trieste/head}}
<html>
<html>
-
      <script type="text/javascript" src="https://ajax.googleapis.com/ajax/libs/jquery/1.7.2/jquery.min.js" ></script>
+
<div id="body" dest_id="home">
-
      <script type="text/javascript" src='/Team:Trieste/static/page_boot.js?action=raw&ctype=text/javascript'></script>
+
<div id="container"> <!-- start container -->
-
      <div id="head">
+
</html>{{Team:Trieste/menu}}<html>
-
          <meta charset="UTF-8">
+
<div id="content"> <!-- start content -->
-
          <title>Team iGEM Trieste 2012</title>
+
<h1 id="h1_lf" class="main_tit"><div>Our Project</div></h1>
 +
<h1 id="h1_mn" class="main_tit"><div>Quick Links</div></h1>
 +
<h1 id="h1_rt" class="main_tit"><div>The Team</div></h1>
 +
<div id="box_left"><!-- start box_main -->
 +
<h2>We present you the Jolly JoCare</h2>
 +
<iframe width="330" height="280" src="http://www.youtube.com/embed/vB5xmbLnj7I" frameborder="0" allowfullscreen></iframe>
 +
<p>
 +
<a href="https://2012.igem.org/Team:Trieste/project/overview" target="_blank">Project Overview</a>
 +
- Have a look to our Jolly JoCare Project at a glance!
 +
</p>
 +
<p>
 +
<strong> Abstract </strong> <br/>
 +
Recent studies have evidenced that having a beneficial and healthy intestinal microflora is very important for human health. Our aim is to modify a bacteria normally found in human gut and create a safe, controllable and versatile molecular platform which can be used to produce a wide range of molecules  leading to a beneficial probiotic. For this purpose we have chosen the <i>E. coli</i> strain Nissle 1917 which has been used for many years as a probiotic. We designed a robust gene guard system regulated by a novel and easy to control inducible cumate switch that activates the production of a human antimicrobial peptide LL-37 that can kill the bacteria and also avoid horizontal transfer. 
 +
The safe probiotic constructed here can be used to produce nutritious, preventive or therapeutic molecules. For example, we have used it to produce an antibody against the emerging virus, Norovirus.
 +
</p>                                 
 +
</div> <!-- end box_left -->
 +
<div id="box_main"> <!-- start box_main -->
 +
<div class="box_contenuti">
 +
<div class="box-article"> <!-- start box-article -->
 +
                       
 +
<!-- -->
 +
<div class="article">
 +
<div class="title">Achievements</div>
 +
<div class="date">  </div>
 +
<div class="intro">
 +
<a href="https://2012.igem.org/Team:Trieste/extra/ach"target="_blank">Quick link</a>
 +
to our judging form and Achievements summary<br/>
 +
</div>
 +
<div class="read_more">
 +
<a href="https://2012.igem.org/Team:Trieste/extra/ach" target="_blank">a</a>
 +
</div>
 +
</div>
 +
                       
 +
<!-- -->
 +
<div class="article">
 +
<div class="title">Main Results</div>
 +
<div class="date">  </div>
 +
<div class="intro">
 +
<a href="https://2012.igem.org/Team:Trieste/project/mainres" target="_blank">Click here</a> to reach our summary page.
 +
</div>
 +
<div class="read_more">
 +
<a href="https://2012.igem.org/Team:Trieste/project/mainres"target="_blank">a</a>
 +
</div>
 +
</div>
 +
<!-- -->
 +
<div class="article">
 +
<div class="title">Data</div>
 +
<div class="date">  </div>
 +
<div class="intro"><a href="https://2012.igem.org/Team:Trieste/data"target="_blank">Here</a> are our favourite parts!</div> </br>
 +
<div class="read_more"><a href="https://2012.igem.org/Team:Trieste/data"target="_blank">a</a></div>
 +
</div>
 +
<br/>
 +
<img src="https://static.igem.org/mediawiki/2012/6/64/Foto_home_cumato_tag.jpg"alt="#" width="300px"/>
 +
</div> <!-- end box-article -->
 +
</div>
 +
</div> <!-- end box_main -->
 +
<div id="box_right"> <!-- start box_right -->
 +
<ul id="sub_menu">
 +
</ul>
 +
<img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" />
 +
<br/>
-
          <link rel="stylesheet" href='/Team:Trieste/static/main.css?action=raw&ctype=text/css' type="text/css" />
+
<div class="box_contacts">
-
      </div>
+
<h2>Contact us</h2>
-
<div id="body">
+
<p>For other information, write to:</p>
-
    <div id=header>
+
<a href="mailto:igem2012@gmail.com" class="btn">igem2012@gmail.com</a>
-
<h3>&nbsp;</h3>
+
<div class="social">
-
<h1>Team iGEM Trieste 2012</h1>
+
Follow us also:
-
<div id="logo"><img src="img/logo.jpg" alt="logo" /></div>
+
<a href="https://www.facebook.com/IGEMUNITS?ref=ts" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/f4/Ico_fb.png" alt="Facebook" class="fb" /></a>
-
    </div>
+
<a href="https://twitter.com/iGEMTrieste" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/21/Ico_twitter.png" alt="twitter" class="tw" /></a>
-
    <div id=menu>&nbsp;</div>
+
</div>
-
    <div id="container">
+
</div>
-
<div id="content">
+
<br/> <br/><br/>
-
    <div class="column">
+
<img src="https://static.igem.org/mediawiki/2012/7/70/Trieste_foto_home_2.jpg" alt="sec photo team trieste" width="230px" height="140" />
-
        <h1>Abstract</h1>
+
 
-
                <h2 style="background-color: white;margin: 0px; padding: 10px;text-align:center;">A safe synthetic probiotic expression system for antibodies.</h2>
+
</div> <!-- end box_right -->
-
<p>Noroviruses (NoV) are cause of epidemic outbreaks of gastrointestinal illness. Even if they are considered as a moderate threat – classified as category B pathogens by the National Institute of Allergy and Infectious diseases<sup><a href="#footnote1">[1]</a></sup> – the spreading speed of these pathogens is dramatic: rates of reported NoV outbreaks reach 21 million cases of acute gastroenteritis per year, 70.000 of those need hospitalization and 800 die<sup><a href="#footnote2">[2]</a></sup>. This is due to a rapid person-to-person transmission in semi-closed places like hospital, school and public transports. Moreover it seems that it is really difficult to develop an immunological memory against NoV that avoids further illness.
+
</html>{{Team:Trieste/footer}}<html>
-
                </p>
+
<!-- end content is included in template -->
-
                <p>
+
</div> <!-- end container -->
-
Our project aims to build a probiotic bacteria that helps the immune system to fight this pathogenic agent. First of all we had to choose a suitable bacterial strain for the gut environment that can easily be manipulated. Than we had to face an important issue: safety. For this purpose we designed a robust gene guard system regulated by a novel inducible switch and by an human antimicrobial peptide that can destroy, in any moment, the bacteria and avoid the transgene horizontal exchange.
+
-
                </p>
+
-
                <p>
+
-
Finally we will provide to our bacteria a weapon able to capture and inactivate our target thanks to a synthetic minibody fused to a protein membrane. This feature can be slightly modified to target any other pathogenic agent with enteric tropism.
+
-
                </p>
+
-
                <hr />
+
-
                <p>
+
-
                    <span id="footnote1">[1] <a href="http://www.niaid.nih.gov/topics/biodefenserelated/biodefense/pages/cata.aspx">http://www.niaid.nih.gov/topics/biodefenserelated/biodefense/pages/cata.aspx</a></span><br />
+
-
                    <span id="footnote2">[2] <a href="http://www.cdc.gov/norovirus/trends-outbreaks.html">http://www.cdc.gov/norovirus/trends-outbreaks.html</a></span>
+
-
                </p>
+
-
    </div>
+
-
</div>
+
-
    </div>
+
</div>
</div>
</html>
</html>

Latest revision as of 02:10, 27 September 2012

Our Project

Quick Links

The Team

We present you the Jolly JoCare

Project Overview - Have a look to our Jolly JoCare Project at a glance!

Abstract
Recent studies have evidenced that having a beneficial and healthy intestinal microflora is very important for human health. Our aim is to modify a bacteria normally found in human gut and create a safe, controllable and versatile molecular platform which can be used to produce a wide range of molecules leading to a beneficial probiotic. For this purpose we have chosen the E. coli strain Nissle 1917 which has been used for many years as a probiotic. We designed a robust gene guard system regulated by a novel and easy to control inducible cumate switch that activates the production of a human antimicrobial peptide LL-37 that can kill the bacteria and also avoid horizontal transfer. The safe probiotic constructed here can be used to produce nutritious, preventive or therapeutic molecules. For example, we have used it to produce an antibody against the emerging virus, Norovirus.

Achievements
Quick link to our judging form and Achievements summary
a
Main Results
Click here to reach our summary page.
a
Data
Here are our favourite parts!

a

#
Team iGEM 2012

Contact us

For other information, write to:

igem2012@gmail.com



sec photo team trieste
Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012
HTML Hit Counter

Retrieved from "http://2012.igem.org/Team:Trieste"