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| - | {| style="color:#1b2c8a;background-color:#3ae2e8;" cellpadding="3" cellspacing="0" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:Cambridge|Home]]
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| - | !align="center"|[[Team:Cambridge/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Cambridge Official Team Profile]
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| - | !align="center"|[[Team:Cambridge/Project|Project]]
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| - | !align="center"|[[Team:Cambridge/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:Cambridge/Modeling|Modeling]]
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| - | !align="center"|[[Team:Cambridge/Notebook|Notebook]]
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| - | !align="center"|[[Team:Cambridge/Safety|Safety]]
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| - | !align="center"|[[Team:Cambridge/Attributions|Attributions]]
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| - | !align="center"|[[Team:Cambridge/Sponsors|Sponsors]]
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| - | |}
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| - | {|align="center"
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| - | !align="center"|[[Team:Cambridge/Week 3|Diary]]
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| - | !align="center"|[[Team:Cambridge/Week 3/Lab book|Lab book]]
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| - | |}
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| - | ===Monday===
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| - |
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| - | Making ''bacillus'' competent
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| - | ----
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| - | '''Bacillus salts''' *10 made up:
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| - | *(NH4)2SO4: 20g
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| - | * KH2PO4 (anhydrous): 60g
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| - | * Anhydrous K2HPO4 189.7g ''(N.B. Different from the one below!)''
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| - | * KH2PO4 60g
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| - | * Tri-sodium citrate 10g
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| - | * MgSO4•7H2O 2g
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| - | * Added distilled water to make 1000mL
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| - | * Autoclaved
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| - | '''Medium A base''' *10 made up:
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| - | * Yeast extract 10g
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| - | * Casamino acids 2g
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| - | * Added distilled water to make 900mL
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| - | * Autoclaved
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| - | ''bacillus'' strain 168 streaked out and grown on plate overnight.
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| - | ===Tuesday===
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| - |
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| - | Making ''bacillus'' competent
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| - | ----
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| - |
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| - | ===Wednesday===
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| - |
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| - | Making ''bacillus'' competent
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| - | ----
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| - |
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| - | ===Thursday===
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| - | Test transformation of frozen ''bacillus'' stocks
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| - | ----
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| - | ===Friday===
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| - | Transformation of ''bacillus'' and e.coli with lux genes from 2010
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| - | ----
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| - | Testing of the spectral properties of filters
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| - | Blue , Green and Red filters were tested in different wavelengths (400 nm - 600 nm) in a spectrophotometer.
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| - | Excellent absorbance/emmitance results given by the blue and red filters near 580nm and 490nm respectively. 580nm is the wavelength where intensity of light is to be measured to identify the presence of mOrange. 490nm is the wavelength where maximum light intensity is expected with or without the presence of mOrange.
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| - | [[File:Filter_spectrum.png|250px||thumb|left]]
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| - |
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| - | [[File:MOrange_expected_graphs.jpg|250px||thumb|left]]
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