Team:Cambridge/Week 3/Lab book

From 2012.igem.org

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{| style="color:#1b2c8a;background-color:#3ae2e8;" cellpadding="3" cellspacing="0" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Cambridge|Home]]
 
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!align="center"|[[Team:Cambridge/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Cambridge Official Team Profile]
 
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!align="center"|[[Team:Cambridge/Project|Project]]
 
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!align="center"|[[Team:Cambridge/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Cambridge/Modeling|Modeling]]
 
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!align="center"|[[Team:Cambridge/Notebook|Notebook]]
 
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!align="center"|[[Team:Cambridge/Safety|Safety]]
 
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!align="center"|[[Team:Cambridge/Attributions|Attributions]]
 
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!align="center"|[[Team:Cambridge/Sponsors|Sponsors]]
 
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|}
 
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{|align="center"
 
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!align="center"|[[Team:Cambridge/Week 3|Diary]]         
 
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!align="center"|[[Team:Cambridge/Week 3/Lab book|Lab book]]
 
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|}
 
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===Monday===
 
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Making ''bacillus'' competent
 
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'''Bacillus salts''' *10 made up:
 
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*(NH4)2SO4: 20g
 
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* KH2PO4 (anhydrous): 60g
 
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* Anhydrous K2HPO4 189.7g ''(N.B. Different from the one below!)''
 
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* KH2PO4 60g
 
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* Tri-sodium citrate 10g
 
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* MgSO4•7H2O 2g
 
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* Added distilled water to make 1000mL
 
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* Autoclaved
 
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'''Medium A base''' *10 made up:
 
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* Yeast extract 10g
 
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* Casamino acids 2g
 
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* Added distilled water to make 900mL
 
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* Autoclaved
 
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''bacillus'' strain 168 streaked out and grown on plate overnight.
 
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===Tuesday===
 
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Making ''bacillus'' competent
 
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===Wednesday===
 
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Making ''bacillus'' competent
 
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===Thursday===
 
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Test transformation of frozen ''bacillus'' stocks
 
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===Friday===
 
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Transformation of ''bacillus'' and e.coli with lux genes from 2010
 
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Testing of the spectral properties of filters
 
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Latest revision as of 13:17, 20 July 2012