Team:Lyon-INSA

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<h3 align=center> 2010 </h3>
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INSA-Lyon -> Silver Medal <br/>
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<h1>Welcome to the Lyon-INSA wiki !</h1>
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<div ><h1>Team project description</h1></div>
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<h3 align=center> 2011 </h3>
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Biofilms are responsible for billions of dollars in production losses and treatment costs in the industry every year. Biofilm-related problems are major concerns in the food industry where it can cause food spoilage or poisoning, in health industry because of pathogens' persistence and dispersal, or in the oil and water industry where it causes corrosion. Assuming that the environment is already over-saturated with harmful chemical products such as biocides, whose long term health effects remain to be elucidated, <b>there is a great need for novel solutions to reduce detrimental biofilm effects</b>.<br/>
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Lyon-INSA-ENS -> Gold Medal and <b> Best New BioBrick Device, Engineered </b> <br/>
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<center><iframe frameborder="0" width="480" height="360" src="http://www.dailymotion.com/embed/video/xtsdia?logo=0"></iframe><br /><a href="http://www.dailymotion.com/video/xtsdia_our-project-presentation-biofilm-killer-lyon-insa-igem_tech" target="_blank"><font color ="purple">Our project presentation Biofilm Killer Lyon...</font></a></center><br>
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<h3 align=center> 2012 </h3>
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<b>To reduce the use of biocides</b>, the Lyon-INSA iGEM team aims to <b>engineer bacterial "torpedo" capable to infiltrate and destroy biofilms</b> formed on industrial equipments, pipes or reservoirs. Industrial surfaces will then be protected from further deleterious contamination by either a <b>surfactant coating</b>, or the <b>establishment of a protective biofilm</b>.<br/>
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Same same.... but different<br/>
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<b>Lyon-INSA is back!!</b> <br/>
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You, <b>sponsor</b>, want to take part in this great adventure?? <br/>
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Our experimental model consists of <b><i>Staphylococcus epidermidis</i> as the detrimental biofilm</b>, and <b><i>Bacillus subtilis</i> as the "Biofilm Killer" agent</b>. Three complementary modules will be constructed to arm our "Biofilm Killer" strain:<br/>
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Download our sponsorship proposal, contact us, and make a step toward an innovative future with us!!! <br/>
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<u>Choose your language:</u>
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<a href="https://static.igem.org/mediawiki/2012/d/d9/Lyon-INSA_plaquette.pdf"><IMG SRC="https://static.igem.org/mediawiki/2012/2/23/Lyon-INSA_UKflag.png" height=3% width=3% TITLE="English version"></a>
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<a href="https://static.igem.org/mediawiki/2012/2/23/Lyon-INSA_plaquettege.pdf"><IMG SRC="https://static.igem.org/mediawiki/2012/4/46/Lyon-INSA_germanflag.png" height=3% width=3% TITLE="German version"></a>
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<div align = center><h1>Team project description</h1></div>
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Biofilms are responsible for billions of dollars in production losses and treatment costs in the industry every year. Biofilm-related problems are major concerns in the food industry where they can cause food spoilage or poisoning, in health industry due to the dispersal of pathogens, or in the oil and water industry where they induce corrosion. Assuming that the environment is already over-saturated with harmful chemical products with indubitable long-term health effects <b>there is a great need for novel solutions to reduce detrimental biofilm effects</b>.<br/>
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<li>The first step will be to fit <i>Bacillus subtilis</i> swimmers with both a <b>biocide and a scattering agent</b>. Irrigation of these active substances in the biofilm should be facilitated by the tunneling activity of these swimmers cells.</li>
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<li>Then, to protect the surface which used to be the biofilm's substrate from next possible bacterial adhesion, we will engineered the <b>conditional production of surfactin</b>, a naturally toxic bio-surfactant produced by <i>B. subtilis</i> and displaying well-known antimicrobial properties.</li>
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<li>Finally, the <b>conditional establishment of a competitive barrier flora</b>, constituted of <i>B. subtilis</i> in our model, will be achieved by inhibiting the main biofilm repressor abrB gene. </li><br/>  
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<b>To reduce the use of biocides</b>, the INSA-Lyon iGEM team aims to <b>engineer “torpedo” bacteria able to destroy biofilms</b> formed on industrial pipes or reservoirs. Industrial piping systems will then be protected from further deleterious contamination by either a <b>surfactant coating</b>, or the <b>establishment of a probiotic biofilm</b>.<br/>
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Our experimental model consists of <b><i>Staphylococcus epidermidis</i> as the detrimental biofilm</b>, and <b><i>Bacillus subtilis</i> as the “Biofilm Killer” agent</b>. Three complementary modules will be constructed to arm our “Biofilm Killer” strain:<br/>
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<li>The first step will be to fit <i>Bacillus subtilis</i> swarmers with both a <b>biocide and a biofilm-distructuring agent</b>. Penetration of these active substances in the biofilm should be facilitated by the swarming activity of these cells.</li>
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<li>In a second step, to prevent surface recolonisation by unwanted biofilms, we will engineer our "Biofilm Killer" strain with a <b>conditional production of surfactin</b>, a naturally toxic bio-surfactant produced by <i>B. subtilis</i> and displaying well-known antimicrobial properties.</li>
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<li>Finally, we will engineer the Bacterial Killer to establish a <b>conditional of a barrier flora</b>, to prevent further surface recolonisation in the long term, by inhibiting the expression of  abrB, its main biofilm formation repressor gene.</li><br/>  
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Bacilli strains are non-pathogenic, and do not cause equipment degradation by corrosion : their attachment on surfaces appears as a good solution to prevent the formation of new dangerous biofilms. This project provides a potential cheap, permanent and environmental-friendly solution for unwanted biofilm development.
 
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<i>Bacillus</i> strains are non-pathogenic, and do not cause equipment degradation by corrosion: their settlement on surfaces represents a  biocide-alternative strategy to prevent the formation of new dangerous biofilm. This project provides a potential economy, and environmental friendly solution for the control of unwanted biofilm.  
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Latest revision as of 22:56, 26 October 2012

Welcome to the Lyon-INSA wiki !

Team project description


Biofilms are responsible for billions of dollars in production losses and treatment costs in the industry every year. Biofilm-related problems are major concerns in the food industry where it can cause food spoilage or poisoning, in health industry because of pathogens' persistence and dispersal, or in the oil and water industry where it causes corrosion. Assuming that the environment is already over-saturated with harmful chemical products such as biocides, whose long term health effects remain to be elucidated, there is a great need for novel solutions to reduce detrimental biofilm effects.


Our project presentation Biofilm Killer Lyon...

To reduce the use of biocides, the Lyon-INSA iGEM team aims to engineer bacterial "torpedo" capable to infiltrate and destroy biofilms formed on industrial equipments, pipes or reservoirs. Industrial surfaces will then be protected from further deleterious contamination by either a surfactant coating, or the establishment of a protective biofilm.

Our experimental model consists of Staphylococcus epidermidis as the detrimental biofilm, and Bacillus subtilis as the "Biofilm Killer" agent. Three complementary modules will be constructed to arm our "Biofilm Killer" strain:

  • The first step will be to fit Bacillus subtilis swimmers with both a biocide and a scattering agent. Irrigation of these active substances in the biofilm should be facilitated by the tunneling activity of these swimmers cells.
  • Then, to protect the surface which used to be the biofilm's substrate from next possible bacterial adhesion, we will engineered the conditional production of surfactin, a naturally toxic bio-surfactant produced by B. subtilis and displaying well-known antimicrobial properties.
  • Finally, the conditional establishment of a competitive barrier flora, constituted of B. subtilis in our model, will be achieved by inhibiting the main biofilm repressor abrB gene.

    • Bacillus strains are non-pathogenic, and do not cause equipment degradation by corrosion: their settlement on surfaces represents a biocide-alternative strategy to prevent the formation of new dangerous biofilm. This project provides a potential economy, and environmental friendly solution for the control of unwanted biofilm.

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