Team:UC Davis/Data

From 2012.igem.org

(Difference between revisions)
 
(26 intermediate revisions not shown)
Line 13: Line 13:
@import "/wiki/skins/igem/main.css?270";
@import "/wiki/skins/igem/main.css?270";
/*]]>*/</style>
/*]]>*/</style>
 +
<link rel="stylesheet" type="text/css" media="screen, projection" href="https://2012.igem.org/wiki/skins/common/shared.css?270" />
<link rel="stylesheet" type="text/css" media="screen, projection" href="https://2012.igem.org/wiki/skins/common/shared.css?270" />
<link rel="stylesheet" type="text/css" media="screen, projection" href="https://2012.igem.org/wiki/skins/igem/main.css?270" />
<link rel="stylesheet" type="text/css" media="screen, projection" href="https://2012.igem.org/wiki/skins/igem/main.css?270" />
Line 66: Line 67:
</script>  
</script>  
<script type="text/javascript" src="https://2012.igem.org/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
<script type="text/javascript" src="https://2012.igem.org/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
<!-- Head Scripts -->
<script src="https://2012.igem.org/wiki/skins/common/ajax.js?270"></script>
<script src="https://2012.igem.org/wiki/skins/common/ajax.js?270"></script>
Line 73: Line 75:
<script type="text/javascript"        src ="https://2012.igem.org/common/jquery-latest.min.js"></script>
<script type="text/javascript"        src ="https://2012.igem.org/common/jquery-latest.min.js"></script>
<script type="text/javascript"        src ="https://2012.igem.org/common/tablesorter/jquery.tablesorter.min.js"></script>
<script type="text/javascript"        src ="https://2012.igem.org/common/tablesorter/jquery.tablesorter.min.js"></script>
 +
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/common/tablesorter/themes/groupparts/style.css" />
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/common/tablesorter/themes/groupparts/style.css" />
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/common/table_styles.css" />
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/common/table_styles.css" />
Line 78: Line 81:
<script type="text/javascript"        src ="https://2012.igem.org/forum/forum_scripts.js"></script>
<script type="text/javascript"        src ="https://2012.igem.org/forum/forum_scripts.js"></script>
 +
<!-- slide script starts here -->
 +
 +
<script type="text/javascript">
 +
function slider() {
 +
    var $current = $('#slides IMG.current');
 +
 +
    if ( $current.length == 0 ) $current = $('#slides IMG:last');
 +
    var $next =  $current.next().length ? $current.next()
 +
        : $('#slides IMG:first');
 +
 +
      $current.addClass('previous');
 +
    $next.css({opacity: 0.0})
 +
        .addClass('current')
 +
        .animate({opacity: 1.0}, 2500, function() {
 +
            $current.removeClass('current previous');
 +
        });
 +
 +
var $navicurrent = $('.progress li.current');
 +
if($navicurrent.length ==0) $navicurrent = $('.progress li:last');
 +
var $navinext = $navicurrent.next().length ? $navicurrent.next() : $('.progress li:first');
 +
$navicurrent.addClass('previous');
 +
$navicurrent.removeClass('current previous');
 +
$navinext.addClass('current').animate({opacity: 1.0}, 2500, function() {
 +
        });
 +
 +
$('.progress li').click(function(){
 +
   
 +
var $ncurrent = $(this);
 +
        $('.progress li').removeClass('current').removeClass('previous');
 +
        $('#slides IMG').removeClass('current').removeClass('previous');
 +
        $ncurrent.addClass('current');
 +
        var ncurrentindex = $ncurrent.index();
 +
if(  ncurrentindex=== 0) $current = $('#slides IMG:first');
 +
        if(  ncurrentindex=== 1) $current = $('#slides IMG:eq(1)');
 +
        if(  ncurrentindex=== 2) $current = $('#slides IMG:eq(2)');
 +
        if(  ncurrentindex=== 3) $current = $('#slides IMG:last');
 +
        $current.addClass('current');
 +
 +
});
 +
 +
}
 +
$(function() {
 +
    setInterval( "slider()", 5000 );
 +
});
 +
 +
$(document).ready(function(){
 +
 +
  $('a.lightbox').click(function(e) {
 +
    $('body').css('display', 'inherit'); // hide scrollbars!
 +
 +
    $('<div id="overlay"></div>')
 +
    .css('opacity', '0')
 +
      .animate({'opacity': '0.7'}, 'slow')
 +
    .click(function(){
 +
      removeLightbox();
 +
      })
 +
      .appendTo('body');
 +
 +
    $('<div id="lightbox"></div>')
 +
      .hide()
 +
      .appendTo('body');
 +
 +
    $('<img>')
 +
      .attr('src', $(this).attr('href'))
 +
      .load(function() {
 +
        positionLightboxImage();
 +
      })
 +
      .click(function() {
 +
        removeLightbox();
 +
      })
 +
      .appendTo('#lightbox');
 +
 +
    return false;
 +
  });
 +
});
 +
 +
function positionLightboxImage() {
 +
  var top = ($(window).height()  - $('#lightbox').height())/2;
 +
  var left = ($(window).width() - $('#lightbox').width())/2;
 +
 +
  $('#lightbox')
 +
    .css({
 +
      'top': top,
 +
      'left':left-80
 +
    })
 +
    .fadeIn();
 +
}
 +
 +
function removeLightbox() {
 +
 +
  $('#overlay, #lightbox')
 +
    .fadeOut('slow', function() {
 +
      $(this).remove();
 +
      $('body').css('overflow-y', 'auto'); // show scrollbars!
 +
    });
 +
}
 +
</script>
 +
<!-- slide script ends here -->
<style type="text/css">
<style type="text/css">
<!-- beginning of slider style -->
<!-- beginning of slider style -->
Line 87: Line 188:
min-height:100%; /* real browsers */
min-height:100%; /* real browsers */
}
}
-
#content { z-index: 1; background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; margin-top: -45px !important;
+
#content { z-index: 1; background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; top:-3px; margin-top: -45px !important;
height:auto !important; /* real browsers */
height:auto !important; /* real browsers */
height:100%; /* IE6: treaded as min-height*/
height:100%; /* IE6: treaded as min-height*/
Line 97: Line 198:
min-height:100%; /* real browsers */
min-height:100%; /* real browsers */
}
}
-
#top-section { z-index: 2; height: 10px; margin: 0px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important; padding:0; border: none; font-size: 10px;}
+
#top-section { z-index: 2; height: 15px; margin: 0px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important; padding:0; border: none; font-size: 10px;}
#p-logo { height:1px; overflow:hidden; display: none;}
#p-logo { height:1px; overflow:hidden; display: none;}
#search-controls { overflow:hidden; display:block; background: none; position: absolute; top: 100px; right: 40px;}
#search-controls { overflow:hidden; display:block; background: none; position: absolute; top: 100px; right: 40px;}
Line 104: Line 205:
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
.right-menu ul { border: none; width: 300px;}
.right-menu ul { border: none; width: 300px;}
-
#footer-box { background-color: #216085; border: none; width: 100%; margin: -10px auto 0 auto; padding: 20px 0;}
+
#footer-box { background-color: #216085; border: none; width: 850px; height: 30px; margin: -10px auto 0 auto; padding: 20px 0;}
.visualClear { display: none; }
.visualClear { display: none; }
#footer { border: none; width: 850px; margin: 0 auto; padding: 0;}
#footer { border: none; width: 850px; margin: 0 auto; padding: 0;}
Line 116: Line 217:
h3#siteSub { display: none;}
h3#siteSub { display: none;}
#contentSub {display: none;}
#contentSub {display: none;}
-
p:first-child { display: none;}
+
p:first-child { display: block;}
h1{border:none; width: 100%; clear: both;}
h1{border:none; width: 100%; clear: both;}
-
<!-- end of slides style -->
 
-
 
-
body {
 
-
    background-color: #ffffff;
 
-
    color: black;
 
-
    font: x-small sans-serif;
 
-
    margin: 0;
 
-
    padding: 0;
 
-
}
 
#globalWrapper {
#globalWrapper {
     position: relative;
     position: relative;
     font-size: 127%;
     font-size: 127%;
-
     width: 100%;
+
     width: 850px //100%;
     margin: 0;
     margin: 0;
     padding: 0;
     padding: 0;
     padding-bottom: 10px;
     padding-bottom: 10px;
-
    background-color: #ffffff;
+
#    background:url(http://img.photobucket.com/albums/v26/bluemelon/bg-2-1.jpg);
 +
#    background-repeat: no-repeat;
 +
#   background-attachment: fixed;
 +
#    background-size:100% auto;
}
}
Line 143: Line 238:
     margin: 0 auto;
     margin: 0 auto;
     padding: 5px 5px 5px 5px;;
     padding: 5px 5px 5px 5px;;
-
     background: white;
+
     background: transparent;
     color: black;
     color: black;
-
    border-left: 1px solid #ffffff;
+
#    border-left: 1px solid #ffffff;
-
    border-right: 1px solid #ffffff;
+
#    border-right: 1px solid #ffffff;
-
    line-height: 1.5em;
+
#    line-height: 1.5em;
-
#    z-index: 2;
+
}
}
 +
 +
 +
body {
 +
background-color: rgba(255,255,255,1);
 +
background-image: url('http://img.photobucket.com/albums/v26/bluemelon/bg-2-1.jpg');
 +
background-size: 100%;
 +
background-repeat: no-repeat;
 +
background-attachment:fixed;
 +
 +
    color: black;
 +
    font: x-small sans-serif;
 +
    margin: 0;
 +
    padding: 0;
 +
#    height:100%;
 +
}
 +
#topmenubar {
#topmenubar {
Line 166: Line 276:
     text-align: right;
     text-align: right;
     text-transform: lowercase;
     text-transform: lowercase;
-
  color: white;
 
}
}
Line 202: Line 311:
.topleft-menu:hover a {
.topleft-menu:hover a {
     color: white;
     color: white;
 +
}
 +
#newnavi {
 +
        position:relative;
 +
        height:42px;
 +
        width: 850px;
 +
        margin-left: -2px;
 +
        display:block;
 +
        background:transparent;
 +
}
 +
#newnavi .newmenu {
 +
        display:inline;
 +
        overflow:hidden;
 +
        padding:0px;
 +
        margin:2px solid transparent;
 +
        list-style-type:none;
 +
        height:24px;
 +
        width: 107px;
 +
        list-style-image:none;
 +
        background: #004b85;
 +
}
 +
#newnavi .newmenu li    {
 +
        float:right;
 +
        background: #004b85;
 +
        text-align:center;
 +
}
 +
#newnavi .newmenu .selected {
 +
        background: #ba9108;
 +
}
 +
#newnavi .newmenu li a  {
 +
text-align:center; vertical-align:middle; width:86px; padding:5px 10px 5px 10px; display:block; color:#fff; text-decoration:none; font:14px arial, verdana, sans-serif; font-weight: bold;
 +
}
 +
#newnavi .newmenu li:hover a {
 +
        text-decoration:none;
 +
}
 +
#newnavi .newmenu ul    {
 +
position:absolute; left:-9999px; top:-9999px; list-style-type:none; list-style-image:none; margin:0px; border:0px; padding:0px; background:  #05bcea;
 +
}
 +
#newnavi .newmenu li:hover      {
 +
position:relative; background: #05bcea;
 +
}
 +
#newnavi .newmenu li:hover ul  {
 +
left:0px; top:26px; background: #05bcea;  width:150px; opacity:1.0 ; z-index:4;
 +
}
 +
#newnavi .newmenu li:hover ul li        {
 +
       
 +
        background: #05bcea;
 +
}
 +
#newnavi .newmenu li:hover ul li a      {
 +
text-align:left; padding:5px 0px 5px 0px;display:block; font-size:12px; width:150px; text-indent:10px; color:#444;
 +
background-color: #05bcea; text-decoration:none;     
 +
}
 +
#newnavi .newmenu li:hover ul li a:hover {
 +
background: #05bcea; color:#ffffff; width:150px;     
}
}
-
#nav {
 
-
    position: relative;
 
-
    height: 24px;
 
-
    width: 850px;
 
-
#    margin-left: 10px;
 
-
#    margin: 0 auto 0 auto;
 
-
#    border-left: 1px solid #444444;
 
-
#    border-right: 1px solid #444444;
 
-
#    border-bottom: 1px solid #444444;
 
-
    background-color: white;
+
#newnavi .newmenu li ul li ul {
-
#    border: 0px solid black;
+
        height:40px;
-
#    height: 0px;
+
        left:299px;
-
#    width: 850px;
+
        top:-26px;
-
#    left: 220px;
+
        margin: 0;
-
#    top: -2px;
+
padding: 0;
-
#    position: relative;}
+
list-style: none;
 +
width: 150px;
 +
        position:absolute;
 +
display:none;
 +
}
-
#nav ul {
+
#newnavi .newmenu li ul li ul li {
-
       
+
position:relative;
-
        list-style-type:none;
+
background: #05bcea;
-
        margin:0;
+
width: 150px;
-
        padding:0;
+
}
-
        overflow:hidden;}
+
 
-
#nav ul .groupb {
+
#newnavi .newmenu li ul li ul li a {
-
    float: right;
+
 
-
    list-style:none;
+
padding:5px 0px 5px 0px;display:block; font-size:12px; width:100px; text-indent:10px; color:#444;
-
    padding: 0 .0em;}
+
background-color: #05bcea; text-decoration:none;
 +
}
 +
 
 +
#newnavi .newmenu li ul li:hover ul {
 +
display:block;
 +
color:#ffffff;  
 +
width:150px;
 +
background:transparent;
 +
}
 +
 
 +
#newnavi .newmenu li ul li:hover ul li {
 +
top:-25px;
 +
left:150px;
 +
margin-bottom:-1px;
 +
height:26px;
 +
}
 +
 
 +
#newnavi .newmenu li ul li:hover ul li a {
-
#nav a:hover, #nav a:focus {
+
padding:5px 0px 5px 0px;display:block; font-size:12px; text-indent:10px; color:#444;
-
        opacity:0.7;
+
background-color: #05bcea; text-decoration:none;border:0px solid;
}
}
#slides {
#slides {
     position:relative;
     position:relative;
-
     height:350px;
+
     height:280px;
     width: 840px
     width: 840px
      
      
Line 259: Line 433:
#slides .previous {
#slides .previous {
     z-index:2;
     z-index:2;
 +
}
 +
 +
.progress {
 +
 +
margin-top:-270px;
 +
margin-left:20px;
 +
position:absolute;
 +
 +
}
 +
 +
.progress li{
 +
border:1px solid rgba(0,0,0,0.9);
 +
border-radius:3px;
 +
margin-left: 5px;
 +
float:left;
 +
display:block;
 +
position:relative;
 +
z-index:10;
 +
background-color:rgba(255,255,255,0.4);
 +
}
 +
 +
.progress li a{
 +
    padding-left: 6px;
 +
    padding-right: 6px;
 +
}
 +
 +
.progress li a:hover {
 +
text-decoration:none;
 +
}
 +
 +
.progress .current {
 +
background-color:white;
 +
}
 +
 +
#myleftrightbox
 +
{
 +
position:relative;
 +
width:850px;
 +
float:left;
 +
background-color:transparent;
 +
margin-top: 0px;
 +
margin-left:3.75px solid transparent;
 +
margin-right:3.75px solid transparent;
 +
border-radius: 4px;
 +
padding: 0px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-1
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-3
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-4
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-1:hover {
 +
background-color:green;
 +
}
 +
#myleftrightbox .fourboxes-2:hover {
 +
background-color:orange;
 +
}
 +
#myleftrightbox .fourboxes-3:hover {
 +
background-color:#05bcea;
 +
}
 +
#myleftrightbox .fourboxes-4:hover {
 +
background-color:#676767;
 +
}
 +
 +
 +
#myleftrightbox .fourboxes2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .spacebox
 +
{
 +
float:left;
 +
width:5px;
 +
 +
}
 +
#myleftbox
 +
{
 +
position:relative;
 +
width:625px;
 +
float:left;
 +
background-color:;
 +
margin-top: 0px;
 +
border-radius: 4px;
 +
padding: 0px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftbox .smallbox
 +
{
 +
width:604px;
 +
float:left;
 +
background-color:#bfbfbf;
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
line-height:1.5em;
 +
color:black;
 +
}
 +
#myleftbox .smallbox h1
 +
{
 +
color:#004b85;
 +
font-weight:bold;
 +
}
 +
#myleftbox .smallbox p
 +
{
 +
color: #004b85;
 +
font-weight:bold;
 +
}
 +
#myleftbox .smallboxsite
 +
{
 +
width:604px;
 +
float:left;
 +
background-color:rgba(191,191,191,0.6 );
 +
margin-top: 17px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
line-height:1.5em;
 +
color:black;
 +
 +
}
 +
#myleftbox .threeboxes
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
border-left:2px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftbox .threeboxes2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
border-radius: 4px;
 +
border-left:2px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftbox .threeleft
 +
{
 +
width:175px;
 +
float:left;
 +
background-color:#ba9108;
 +
margin-top: -52px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftbox .threecenter
 +
{
 +
width:175px;
 +
#float:left;
 +
background-color:#ba9108;
 +
margin-top: -52px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftbox .threeright
 +
{
 +
width:175px;
 +
float:right;
 +
background-color:#ba9108;
 +
margin-top: -52px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
 +
 +
#myleftbox .twoboxes
 +
{
 +
width:295px;
 +
height:120px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
border-left:2px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftbox .twoboxes1
 +
{
 +
width:295px;
 +
height:120px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
border-left:1px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
left:9px;
 +
}
 +
 +
#myleftbox .twoboxes:hover {
 +
background-color:red;
 +
}
 +
 +
#myleftbox .twoboxes1:hover {
 +
background-color:green;
 +
}
 +
 +
#sponsorbox
 +
{
 +
position: relative;
 +
width: 208px;
 +
margin-top:15px;
 +
float: right;
 +
height: auto;
 +
}
 +
 +
#sponsorbox .sponsorfloat
 +
{
 +
left:-3px;
 +
width:198px;
 +
float: right;
 +
background-color: #004b85;
 +
margin-top: 0px;
 +
margin-bottom:5px;
 +
border-radius: 4px;
 +
padding: 5px 5px 5px 5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: rgba(225,225,225,1);
 +
}
 +
 +
#sponsorbox .sponsorfloat h2
 +
{
 +
#color:#ba9108;
 +
color:white;
 +
font-size:130%;
 +
}
 +
 +
#sponsorbox .sponsorfloat a
 +
{
 +
color:white;
 +
font: sans-serif;
 +
}
 +
 +
#sponsorbox .sponsorfloat p
 +
{
 +
font: sans-serif;
 +
}
 +
.newsAnnouncement
 +
{
 +
width:205px;
 +
height:110px;
 +
overflow-x:hidden;
 +
overflow-y:scroll;
}
}
Line 265: Line 813:
position:relative;
position:relative;
top: 0px;
top: 0px;
-
width:830px;
+
width:790px;
left: 0px;
left: 0px;
background-color: #d2d2d2;
background-color: #d2d2d2;
margin-top: 0px;
margin-top: 0px;
border-radius: 4px;
border-radius: 4px;
-
padding: 30px 10px 10px 10px;
+
padding: 30px;
font: sans-serif;
font: sans-serif;
font-size: 13px;
font-size: 13px;
Line 287: Line 835:
font-weight: bold;
font-weight: bold;
color: #016D8B;
color: #016D8B;
 +
}
 +
 +
.floatbox3 article{
 +
font-size: 14px;
 +
color: #000000;
}
}
Line 302: Line 855:
     padding: 0 .0em;}
     padding: 0 .0em;}
-
 
+
#catlinks {
 +
background-color:transparent;
 +
border:1px solid transparent;
 +
}
 +
#footpage {
 +
background-color:transparent;
 +
}
 +
#footer-box {
 +
background-color:rgba(191,191,191,0.5);
 +
margin-top:0px;
 +
}
#footer-box-1 {
#footer-box-1 {
          
          
Line 311: Line 874:
         border: 1px solid #444444;
         border: 1px solid #444444;
}
}
 +
#tweets {
 +
    width: 203px;
 +
    margin: 0 auto;
 +
    font: sans-serif;
 +
    font-size: 12px;
 +
}
 +
 +
#tweets .twtr-widget,
 +
#tweets .twtr-doc {
 +
 +
    width: 100%;
 +
    height: auto;
 +
}
 +
 +
#tweets .twtr-hd {
 +
    display:none;
 +
    background: #d40;
 +
    color: #fff;
 +
    -moz-border-radius: 4px 4px 0 0;
 +
    border-radius: 4px 4px 0 0;
 +
    font-family: sans-serif !important;
 +
    }
 +
 +
#tweets .twtr-hd *,
 +
#tweets .twtr-hd h4 a {
 +
 +
    background: #d40 !important;
 +
    font: sans-serif !important;
 +
}
 +
 +
#tweets .twtr-hd h3,
 +
#tweets .twtr-hd h4 {
 +
 +
    font-weight: normal;
 +
    text-align: left;
 +
    margin:0;
 +
}
 +
 +
#tweets .twtr-hd h3 {
 +
       
 +
    background: #fff !important;
 +
    color: #333 !important;
 +
    font: sans-serif;
 +
    font-size: 1.0em !important;
 +
    padding: 0.2em !important;
 +
    -moz-border-radius: 0px;
 +
    border-radius: 0px;
 +
 +
}
 +
 +
#tweets .twtr-tweet {
 +
 +
    background: #fff;
 +
    font: sans-serif !important;
 +
    font-size: 12px;
 +
}
 +
 +
#tweets .twtr-tweet a:link,
 +
#tweets .twtr-tweet a:visited,
 +
#tweets .twtr-tweet a:hover {
 +
 +
    color: #c40 !important;
 +
 +
}
 +
 +
#tweets .twtr-tweet .twtr-tweet-text {
 +
 +
    border-bottom: 1px dashed #666 !important;
 +
    padding-bottom: 4px !important;
 +
    font: sans-serif;
 +
    font-size:12px;
 +
}
 +
 +
#tweets .twtr-tweet .twtr-tweet-text p {
 +
 +
    color: #333 !important;
 +
    font: sans-serif;
 +
    font-size: 12px;
 +
 +
}
 +
 +
#overlay {
 +
  z-index:2;
 +
  position:fixed;
 +
  top: 0;
 +
  left: 0;
 +
  height: 100%;
 +
  width: 100%;
 +
  background: black no-repeat scroll center center;
 +
}
 +
 +
#lightbox {
 +
  position: fixed;
 +
  width:840;
 +
  z-index:2;
 +
}
</style>
</style>
Line 327: Line 986:
  <li style='color:#014457; cursor:default'><a>teams</a></li>
  <li style='color:#014457; cursor:default'><a>teams</a></li>
                    <li class='selected'        ><a href="https://2012.igem.org/Team:UC_Davis/Data">Page</a></li>
                    <li class='selected'        ><a href="https://2012.igem.org/Team:UC_Davis/Data">Page</a></li>
 +
                 <li class='new'><a href="https://2012.igem.org/wiki/index.php?title=Talk:Team:UC_Davis/Data&amp;action=edit&amp;redlink=1">Discussion</a></li>
                 <li class='new'><a href="https://2012.igem.org/wiki/index.php?title=Talk:Team:UC_Davis/Data&amp;action=edit&amp;redlink=1">Discussion</a></li>
               <li><a href="https://2012.igem.org/wiki/index.php?title=Team:UC_Davis/Data&action=edit">Edit</a></li>
               <li><a href="https://2012.igem.org/wiki/index.php?title=Team:UC_Davis/Data&action=edit">Edit</a></li>
Line 336: Line 996:
    </ul>
    </ul>
  </div>
  </div>
-
   
+
  <div id="newnavi">
-
      <div id="nav">
+
    <ul class="newmenu">
-
        <ul>
+
         <li ><a href="https://2012.igem.org/" title="Back to iGEM">iGEM</a></li>
-
         <li class="groupb"><a href="https://2012.igem.org/" title="Back to iGEM">
+
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_09.jpg"></a></li>
+
-
         <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Attributions" title="Attributions">
+
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Attributions" title="Attributions">Attributions</a></li>
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_08.jpg"></a></li>
+
-
         <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Data" title="Data">
+
         <li class="selected"><a href="https://2012.igem.org/Team:UC_Davis/Data" title="Data">Data</a>
-
<img src="https://static.igem.org/mediawiki/2012/5/5a/UCDMain-button_07selected.jpg"></a></li>
+
          <ul>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Data" title="Data">Data</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Parts">Parts</a></li>
 +
          </ul>
 +
        </li>
 +
        <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Notebook">Notebook</a>
 +
          <ul>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook">Notebook</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols ">Protocols</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Gallery">Gallery</a></li>
 +
          </ul>
 +
        </li>
-
         <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Notebook">
+
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Safety" title="Safety">Safety</a></li>
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_06.jpg"></a></li>
+
-
         <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Safety" title="Safety">
+
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Project" title="Project">Project</a>
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_05.jpg"></a></li>
+
          <ul>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Project">Project Overview</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Catalyst">Module Engineering</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Protein_Engineering">Protein Engineering</a></li>
-
        <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Project" title="Project">
+
            <li ><a title="https://2012.igem.org/Team:UC_Davis/Project/Strain">Chassis Engineering </a>
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_04.jpg"></a></li>
+
  <ul>
 +
                <li><a href="https://2012.igem.org/Team:UC_Davis/Project/Strain">Background</a></li>
 +
        <li><a href="https://2012.igem.org/Team:UC_Davis/Project/Directed_Evolution">Directed Evolution</a></li>
 +
                <li><a href="https://2012.igem.org/Team:UC_Davis/Project/Our_Strain">Rational Engineering </a></li>
 +
</ul>
 +
</li>
 +
          </ul>
 +
        </li>
 +
        <li ><a href="https://2012.igem.org/Team:UC_Davis/Team" title="Team">Team</a></li>
 +
        <li ><a href="https://2012.igem.org/Team:UC_Davis" title="Home">Home</a></li>
 +
    </ul>
 +
  </div>
 +
<!-- slide show starts here
 +
<div id="slides">
-
        <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis/Team" title="Team">
+
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-1-2.jpg" width="850" height="280" alt="" class="current" />
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/main-button_03.jpg"></a></li>
+
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-2-2.jpg" width="850" height="280" alt="" />
 +
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-3-2.jpg" width="850" height="280" alt="" />
 +
    <img src="http://img.photobucket.com/albums/v26/bluemelon/slide-4-2.jpg" width="850" height="280" alt="" />
 +
</div>
 +
    <ul class="progress">
 +
<li class="current"><a href="#n_0">1</a> </li>
 +
<li><a href="#n_1">2</a> </li>
 +
        <li><a href="#n_2">3</a> </li>
 +
        <li><a href="#n_3">4</a> </li>
 +
    </ul>
 +
slide show ends here -->  
-
        <li class="groupb"><a href="https://2012.igem.org/Team:UC_Davis" title="Home">
+
<img src="https://static.igem.org/mediawiki/2012/e/ee/UCD_data_banner.jpg">
-
<img src="https://static.igem.org/mediawiki/2012/c/c4/UCDMain-button_02normal.jpg"></a></li>
+
-
        </ul>
+
-
      </div> 
+
-
<br>
+
-
<!--<a><h1 class="firstHeading">Team:UC Davis</h1></a>-->
+
-
<div id="bodyContent">  
+
<!-- <div id="sponsorbox" style="width:220px; float:right;"> -->
-
            <div id="contentSub"></div>
+
 
-
<br>
+
<div id="sponsorbox">
 +
<div id="sponsorbox" class="sponsorfloat">
 +
<!-- twitter starts here -->
 +
</script>
 +
<div id="tweets">
 +
<center>
 +
<h2>UCDavis iGEM Tweets</h2>
 +
</center>
 +
<script charset="utf-8" src="http://widgets.twimg.com/j/2/widget.js"></script>
 +
<script>
 +
new TWTR.Widget({
 +
  version: 2,
 +
  type: 'profile',
 +
  rpp: 10,
 +
  interval: 30000,
 +
  width: 203,
 +
  height: 100,
 +
 
 +
  theme: {
 +
    shell: {
 +
      background: '#004b85',
 +
      color: '#ffffff'
 +
    },
 +
    tweets: {
 +
      background: '#004b85',
 +
      color: '#ffffff',
 +
      links: '#4aed05'
 +
    }
 +
 +
  },
 +
  features: {
 +
    scrollbar: true,
 +
    loop: false,
 +
    live: true,
 +
    behavior: 'all'
 +
  }
 +
}).render().setUser('UCDavisiGEM').start();
-
<div class="floatbox3">
+
</script>
-
<h1> Data </h1>
+
</div>
</div>
-
<p>Here goes our data</p>
+
<!-- twitter ends here -->
-
<!--
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #c2c2c2; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #c1c1c1; padding: 5px;">
+
 
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples.
+
<div id="sponsorbox" class="sponsorfloat">
 +
<center>
 +
<h2>Our Sponsors</h2>
 +
<a href="http://www.novozymes.com/en/Pages/default.aspx" target="_blank"><img src="https://static.igem.org/mediawiki/2011/2/21/UCD_Novozymes-logo.jpg" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://engineering.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/f/f6/UCD_CoE.png" width="200" height="40"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://biosci.ucdavis.edu/index_js.html" target="_blank"><img src="https://static.igem.org/mediawiki/2011/b/b1/UCD_biosci_sponsor.jpg" width="200" height="90"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.genomecenter.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/1/1b/UCD_Genome_center_sponsor.jpg" width="200" height="60"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.cs.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/6/6b/UCD_Computer_sponsor.jpg" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.bme.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/4/40/UCD_BME_logo_minimal_copy.png" width="200 height="70"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.fishersci.com" target="_blank"><img src="https://static.igem.org/mediawiki/2011/a/a4/UCD_Fisher_Logo.gif" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.arcadiabio.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/4/46/UCD_Arcadia_sponsor.jpg
 +
" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://provost.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/8/82/UCD_Provost_sponsor.jpg
 +
" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://www.research.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/9/99/UCD_Research_sponsor.jpg" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a href="http://ucomm.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/b/b4/UCD_Communications_sponsor.jpg" width="200"></a>
 +
</center>
 +
 
 +
<center>
 +
<a title="" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/25/UCD_Schultz_sponsor.jpg
 +
" width="200"></a>
 +
</center>
 +
 
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #cccccc; padding: 5px;">
 
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
 
</div>
</div>
 +
<!--  <br> -->
 +
<!-- div id="myleftbox" style="width:625px; float:left;" -->
 +
<div id="myleftbox">
 +
<div id="myleftbox"  class="smallbox">
 +
<h1>Data</h1>
 +
<article>
 +
The accumulation of plastic products poses a hazard to the environment, as well as humans, through drinking water contamination. This threat led us to develop a degradation pathway to turn the polyethylene terephthalate into different substrates. We produce terephthalic acid and ethylene glycol. Ethylene glycol is a moderately toxic substance, which is oxidized to glycolic acid. The glycolic acid is further oxidized to oxalic acid – a toxic substance that affects the central nervous system via the liver. However, in the environment, the ethylene glycol will be degraded by hydroxyl radicals and in sewage sludge, it is readily biodegradable. Because ethylene glycol must be ingested to pose a problem, researchers take extra precaution to make sure there are no splashes of ethylene glycol in the laboratory and the wastes will be disposed of in the appropriate hazardous waste receptacles. Ethylene glycol can also be a mild irritant if it comes in contact with the skin or if it is inhaled, so researchers wear eye protection as well as gloves and lab coats, and always work with ethylene glycol in the confine of a fume hood. Also, in our constructs, we have produced enzymes that will degrade ethylene glycol into glycoaldehyde and then glycolate. The glycolate has the potential to be turned in to oxaloacetate, a metabolic intermediate. In the environment, ethylene glycol can potentially be toxic within waterways, however the team made sure to dispose of ethylene glycol in a responsible way.
 +
<br><br><a name="Growth">
 +
<p>Toxicity of EG Graph for MG1655 and DH5a</p></a>
 +
We observed that EG is non-toxic to both DH5a and MG1655 cells, as evident from the growth of the two strains. Both strains were exposed to LB media containing varying amounts of EG, ranging from 0 mM to 150 mM.
-
<p><br />
+
<br><center>
-
</p><p><br />
+
<a href="https://static.igem.org/mediawiki/2012/d/d3/UCD_DH5a_data_1_large.jpg" class="lightbox">
-
</p>
+
<img src="https://static.igem.org/mediawiki/2012/2/2b/UCD_DH5a_data_1.jpg" width="300" align="left"></a>
-
<table align="justify">
+
<a href="https://static.igem.org/mediawiki/2012/7/7e/UCD_MG1655_data_1_large.jpg" class="lightbox">
-
<tr>
+
<img src="https://static.igem.org/mediawiki/2012/4/43/UCD_MG1655_data_1.jpg" width="300" align="right"></a>
-
<td>You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
</center>
-
</td><td><div class="thumb tright"><div class="thumbinner" style="width:182px;"><a href="https://2012.igem.org/wiki/index.php?title=Special:Upload&amp;wpDestFile=UC_Davis_logo.png" class="new" title="File:UC Davis logo.png">File:UC Davis logo.png</a>  <div class="thumbcaption">200px</div></div></div>
+
<br><br><br><br><br><br><br><br><br><br>
-
</td></tr>
+
<br><br><br><br><br><br><br><br><br><br>
-
<tr>
+
-
<td>
+
-
<p><i>Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)</i>
+
-
</p>
+
-
</td><td><div class="thumb tright"><div class="thumbinner" style="width:182px;"><a href="/wiki/index.php?title=Special:Upload&amp;wpDestFile=UC_Davis_team.png" class="new" title="File:UC Davis team.png">File:UC Davis team.png</a>  <div class="thumbcaption">Your team picture</div></div></div>
+
-
</td></tr>
+
-
<tr>
+
-
<td>
+
-
</td><td align="center"><strong class="selflink"> Team UC_Davis</strong>
+
-
</td></tr></table>
+
-
<p><br />
+
-
</p>
+
-
<table style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" width="62%" align="center">
+
-
<tr>
+
-
<th align="center"><strong class="selflink">Home</strong>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Team" title="Team:UC Davis/Team">Team</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team.cgi?year=2012&amp;team_name=UC_Davis" class="external text" rel="nofollow">Official Team Profile</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Project" title="Team:UC Davis/Project">Project</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Parts" title="Team:UC Davis/Parts">Parts Submitted to the Registry</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Modeling" title="Team:UC Davis/Modeling">Modeling</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Team:UC Davis/Notebook">Notebook</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Safety" title="Team:UC Davis/Safety">Safety</a>
+
-
</th><th align="center"><a href="https://2012.igem.org/Team:UC_Davis/Attributions" title="Team:UC Davis/Attributions">Attributions</a>
+
-
</th></tr></table>
+
-
-->
+
-
<!--
+
-
NewPP limit report
+
-
Preprocessor node count: 4/1000000
+
-
Post-expand include size: 0/2097152 bytes
+
-
Template argument size: 0/2097152 bytes
+
-
Expensive parser function count: 0/100
+
-
-->
+
-
<!-- Saved in parser cache with key 2012_igem_org:pcache:idhash:1318-0!1!0!!en!2!edit=0 and timestamp 20120705172055 -->
+
 
-
<!--
+
<p>Spain in EG Graph</p>
 +
Knowing that Strain E-15 EG3 utilizes ethylene glycol, we devised an experiment to test the optimal amount of ethylene glycol the strain would thrive in. From the graph, we deduced that 30 mM of ethylene glycol showed the highest growth rate, which also matched previous data provided in the referenced paper [1].
 +
<br>
 +
<a href="https://static.igem.org/mediawiki/2012/3/3b/UCD_EG_large_1.jpg" class="lightbox"><img src="https://static.igem.org/mediawiki/2012/6/67/UCD_EG_1.jpg"></a>
 +
 
 +
</article></div>
 +
<br>
 +
<div id="myleftbox"  class="smallbox">
 +
<h1>References</h1>
 +
<article>
 +
1. Boronat, Albert, Caballero, Estrella, and Juan Aguilar. “Experimental Evolution of a Metabolic Pathway for Ethylene Glycol Utilization by Escherichia coli.” Journal of Bacteriology, Vol. 153 No. 1, pp. 134-139, January 1983.)
 +
</article></div>
 +
 
 +
 
 +
<!-- site map starts here -->
 +
<div id="myleftbox"  class="smallboxsite">
 +
<ul style="font-size:10px;list-style-image:none;list-style-type:none;float:left;display:inline;color:#000000;"  >
 +
 
 +
<li style="float:left;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis"><p>Home</p><ul style="text-indent:-15px;
 +
list-style-image:none;list-style-type:none;color:#000000;"><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis">Welcome</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis">Tweets</a></li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis">Sponsors</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Criteria">Criteria</a> </li> </ul> </a> </li>
 +
 
 +
<li style="float:left;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Team"><p>Team</p><ul style="text-indent:-15px;
 +
list-style-image:none;list-style-type:none;color:#000000;"><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Team">Who we are</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Team">Students</a></li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Team">Advisors</a> </li> </ul> </a> </li>
 +
 
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Project "><p>Project</p></a> <ul style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000"><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project">Project Overview</a> </li>
 +
<li><a style="color:#000000 "  href="https://2012.igem.org/Team:UC_Davis/Project/Catalyst ">Module Engineering</a></li>
 +
<li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Protein_Engineering ">Protein Engineering</a></li>
 +
<li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Strain ">Chassis Engineering</a></li>
 +
  <li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Directed_Evolution "> - Directed Evolution</a></li>
 +
  <li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Our_Strain "> - Rational Engineering</a></li>
 +
<li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Criteria">Critera</a> </li></ul> </li>
 +
 
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Team:UC_Davis/Safety "> <p>Safety</p></a> <a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Safety "> Safety</a> </li>
 +
 
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Notebook "> <p>Notebook</p></a> <ul style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000  "><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook">Notebook</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols ">Protocols</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook/Gallery">Gallery</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Criteria">Critera</a> </li> </ul> </li>
 +
 
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Data "> <p>Data </p></a> <ul style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000  "><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Data "> Data</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Parts ">Parts</a></li> </ul>
 +
 
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Team:UC_Davis/Attributions "> <p>Attribution </p></a><a style="color:#000000 "  href="https://2012.igem.org/Team:UC_Davis/Attributions "> Attribution</a></li>
 +
 
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Main_Page "> <p>iGEM </p></a><a style="color:#000000 " href="https://2012.igem.org/Main_Page " > iGEM</a></li>
 +
</ul>
 +
</div>
 +
<!-- site map ends here -->
 +
 
 +
</div>
 +
</div>
 +
<!--  </div> -->
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
</div>
 +
</body>
 +
<!-- footer starts here -->
 +
<br>
 +
<div id="footpage">
<div class="printfooter">
<div class="printfooter">
Retrieved from "<a href="https://2012.igem.org/Team:UC_Davis">https://2012.igem.org/Team:UC_Davis</a>"</div>
Retrieved from "<a href="https://2012.igem.org/Team:UC_Davis">https://2012.igem.org/Team:UC_Davis</a>"</div>
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div>
+
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
-
-->
+
<div class="visualClear"></div>
-
<!-- end content -->
+
-
<!-- <div class="visualClear"></div>  -->
+
</div>
</div>
     </div>
     </div>
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
<script>if (window.runOnloadHook) runOnloadHook();</script>
</div>
</div>
-
<!-- Served in 0.192 secs. -->
+
</div>
-
</body>
+
<!-- footer ends here -->
</html>
</html>

Latest revision as of 20:55, 3 October 2012

Team:UC Davis - 2012.igem.org

UCDavis iGEM Tweets

Our Sponsors

Data

The accumulation of plastic products poses a hazard to the environment, as well as humans, through drinking water contamination. This threat led us to develop a degradation pathway to turn the polyethylene terephthalate into different substrates. We produce terephthalic acid and ethylene glycol. Ethylene glycol is a moderately toxic substance, which is oxidized to glycolic acid. The glycolic acid is further oxidized to oxalic acid – a toxic substance that affects the central nervous system via the liver. However, in the environment, the ethylene glycol will be degraded by hydroxyl radicals and in sewage sludge, it is readily biodegradable. Because ethylene glycol must be ingested to pose a problem, researchers take extra precaution to make sure there are no splashes of ethylene glycol in the laboratory and the wastes will be disposed of in the appropriate hazardous waste receptacles. Ethylene glycol can also be a mild irritant if it comes in contact with the skin or if it is inhaled, so researchers wear eye protection as well as gloves and lab coats, and always work with ethylene glycol in the confine of a fume hood. Also, in our constructs, we have produced enzymes that will degrade ethylene glycol into glycoaldehyde and then glycolate. The glycolate has the potential to be turned in to oxaloacetate, a metabolic intermediate. In the environment, ethylene glycol can potentially be toxic within waterways, however the team made sure to dispose of ethylene glycol in a responsible way.

Toxicity of EG Graph for MG1655 and DH5a

 We observed that EG is non-toxic to both DH5a and MG1655 cells, as evident from the growth of the two strains. Both strains were exposed to LB media containing varying amounts of EG, ranging from 0 mM to 150 mM.




















Spain in EG Graph

Knowing that Strain E-15 EG3 utilizes ethylene glycol, we devised an experiment to test the optimal amount of ethylene glycol the strain would thrive in. From the graph, we deduced that 30 mM of ethylene glycol showed the highest growth rate, which also matched previous data provided in the referenced paper [1].

References

1. Boronat, Albert, Caballero, Estrella, and Juan Aguilar. “Experimental Evolution of a Metabolic Pathway for Ethylene Glycol Utilization by Escherichia coli.” Journal of Bacteriology, Vol. 153 No. 1, pp. 134-139, January 1983.)

Retrieved from "http://2012.igem.org/Team:UC_Davis/Data"