Team:LMU-Munich/Spore Coat ProteinsTour/Background

From 2012.igem.org

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|[[File:Imamura, 2011 & McKenney, 2010.png|Protein distribution in spore coat of ''Bacillus subtilis''|500px|center]]
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=====Crust Protein Genes Organization=====
=====Crust Protein Genes Organization=====
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<p align="justify">The gene ''cgeA'' is located in the ''cgeABCDE'' cluster and is expressed from its own promoter P<sub>''cgeA''</sub>. The cluster ''cotVWXYZ'' contains the gene ''cotZ'' which is cotranscribed with ''cotY'' and expressed from the promoter P<sub>''cotYZ''</sub>. Another promoter of this cluster, P<sub>''cotV''</sub>, is responsible for the transcription of the other three genes (Fig. 2). Those three promoters were evaluated with the ''lux'' reporter genes ([http://partsregistry.org/Part:BBa_K823025 pSB<sub>''Bs''</sub>3C-''lux''ABCDE]) to analyze their strength and the time point of their activation (see [https://2012.igem.org/Team:LMU-Munich/Data/crustpromoters data]).</p>  
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<p align="justify">The gene ''cgeA'' is located in the ''cgeABCDE'' cluster and is expressed from its own promoter P<sub>''cgeA''</sub>. The cluster ''cotVWXYZ'' contains the gene ''cotZ'' which is cotranscribed with ''cotY'' and expressed from the promoter P<sub>''cotYZ''</sub>. Another promoter of this cluster, P<sub>''cotV''</sub>, is responsible for the transcription of the other three genes (Fig. 2). Those three promoters were evaluated with the ''lux'' reporter genes (pSB<sub>''Bs''</sub>3C-''lux''ABCDE) to analyze their strength and the time point of their activation (visit later DATA page). Based on this data, two of the three promoters could be used for expression of spore crust fusion proteins.
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Based on this data, two of the three promoters could be used for expression of spore crust fusion proteins.
 

Latest revision as of 16:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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