Team:LMU-Munich

From 2012.igem.org

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==='''Bead'''zillus: Fundamental BioBricks for ''Bacillus subtilis'' and spores as a platform for protein display===
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|[[File:NEXT.png|100px|link=Team:LMU-Munich/Abstract]]
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|'''Bead'''zillus - For a guided tour through the most important information about our project and results, klick [[Team:LMU-Munich/Abstract|here]].
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/Abstract]]  
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|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
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|[[Team:LMU-Munich/Why_Beadzillus|Why is there a need for our Project '''Bead'''zillus?]]  
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|Why is there a need for our Project [[Team:LMU-Munich/Why_Beadzillus|'''Bead'''zillus]]?
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|[[File:LMU_The_solution.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
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|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].  
|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].  
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|[[Finally, we succeeded in displaying GFP on Endospores. '''Sporo'''beads.  
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|Finally, we succeeded in displaying proteins on endospores. Our [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]].  
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] thinkable.
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] are imaginable.
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==='''Bead'''zillus: Fundamental BioBricks for ''Bacillus subtilis'' and spores as a platform for protein display===
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We chose to work with [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction <i>Bacillus subtilis</i>] to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox] (<b>B<sup>4</sup></b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress-resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>B<sup>4</sup></b>, we developed [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins <b>Sporo</b>beads]. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector <b>Sporo</b>vector] to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we [https://2012.igem.org/Team:LMU-Munich/Germination_Stop prevented germination] by knocking out involved genes and developed a [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Suicide</b>switch] turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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Latest revision as of 13:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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