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Team/CINVESTAV-IPN-UNAM MX/Chassis.htm
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<h1><em>Chassis!</em></h1> | <h1><em>Chassis!</em></h1> | ||
</br> | </br> | ||
| - | <p>Rhodopseudomonas palustris has an extraordinary metabolic versatility; this microorganism is able | + | <p><em>Rhodopseudomonas palustris</em> has an extraordinary metabolic versatility; this microorganism is able |
| - | to grow in a wide variety of environmental conditions. R palustris obtain energy by different | + | to grow in a wide variety of environmental conditions. <em>R palustris</em> obtain energy by different |
mechanism including anoxygenic photosynthesis, aerobic and anaerobic respiration<sup>1.</sup></p> | mechanism including anoxygenic photosynthesis, aerobic and anaerobic respiration<sup>1.</sup></p> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2012/d/d7/Cha01.gif" alt="cha01" width="324" height="363"></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2012/d/d7/Cha01.gif" alt="cha01" width="324" height="363"></div> | ||
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<p>When O2 tension is little, light energy can be harnessed by a photosynthetic electron transport | <p>When O2 tension is little, light energy can be harnessed by a photosynthetic electron transport | ||
chain that has similar features to those used by plants and other oxygen-evolving organisms (3). | chain that has similar features to those used by plants and other oxygen-evolving organisms (3). | ||
| - | During photosynthetic growth, R. palustris is capable of use either carbon dioxide (CO2) or organic | + | During photosynthetic growth, <em>R. palustris</em> is capable of use either carbon dioxide (CO2) or organic |
carbon sources.</p> | carbon sources.</p> | ||
<p id="text2">Biotechnological potential</p> | <p id="text2">Biotechnological potential</p> | ||
| - | <p>R. palustris is an excellent chassis because is useful in a wide variety of biotechnological | + | <p><em>R. palustris</em> is an excellent chassis because is useful in a wide variety of biotechnological |
applications because of its regulation systems that allow it to sense environmental conditions.</p> | applications because of its regulation systems that allow it to sense environmental conditions.</p> | ||
<img src="https://static.igem.org/mediawiki/2012/6/65/Cha02.gif" alt="cha02" width="557" height="376"><br> | <img src="https://static.igem.org/mediawiki/2012/6/65/Cha02.gif" alt="cha02" width="557" height="376"><br> | ||
| - | <p id="text2"><br>Why synthetic biology in | + | <p id="text2"><br>Why synthetic biology in <em>Rhodopseudomonas palustris</em>? |
</p> | </p> | ||
| - | <p>The Synthetic Biology application in R. palustris could help to harness its biotechnological | + | <p>The Synthetic Biology application in <em>R. palustris</em> could help to harness its biotechnological |
potential.<br> | potential.<br> | ||
We want to investigate the functioning of 2 orthologous regulation systems that respond to | We want to investigate the functioning of 2 orthologous regulation systems that respond to | ||
| - | oxygen and light inspired in Rhodobacter sphaeroides regulatory systems.<br> | + | oxygen and light inspired in <em>Rhodobacter sphaeroides</em> regulatory systems.<br> |
Also we aim to generate a set of regulatory biobricks for Purple Non-Sulfur Photosynthetic | Also we aim to generate a set of regulatory biobricks for Purple Non-Sulfur Photosynthetic | ||
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<hr> | <hr> | ||
| - | <p id="refe">References<br>1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) The Purple Phototrophic Bacteria. Springer; | + | <p id="refe">References<br>1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) <strong>The Purple Phototrophic Bacteria</strong>. Springer; |
200928. pp. 707–725. <br> | 200928. pp. 707–725. <br> | ||
| - | 2. Harwood et. al. (2004) Complete genome sequence of the metabolically versatile photosynthetic | + | 2. Harwood et. al. (2004) <strong>Complete genome sequence of the metabolically versatile photosynthetic |
| - | bacterium Rhodopseudomonas palustris | + | bacterium <em>Rhodopseudomonas palustris</em></strong> Nature Biotechnology Volume 2, Number 1, January 2004<br> |
| - | 3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J. Noguera D., Donohue T. (2011) iRsp1095: A genome- | + | 3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J. Noguera D., Donohue T. (2011) <strong>iRsp1095: A genome- |
| - | scale reconstruction of | + | scale reconstruction of the <em>Rhodobacter sphaeroides</em> metabolic network</strong> BMC Systems Biology 2011, 5:116 |
</p> | </p> | ||
</div> | </div> | ||
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<!-- end #page --> | <!-- end #page --> | ||
<div id="piedepagina"> | <div id="piedepagina"> | ||
| - | <p align="center"> Rhodofactory 2012 </p> | + | <p align="center"> <strong>Rhodofactory 2012</strong> </p> |
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div> |
</div> | </div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| Line 366: | Line 366: | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="80" height="97" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height="91" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="70" height="96" /></div> |
</div> | </div> | ||
Latest revision as of 15:03, 26 October 2012
Chassis!
Rhodopseudomonas palustris has an extraordinary metabolic versatility; this microorganism is able to grow in a wide variety of environmental conditions. R palustris obtain energy by different mechanism including anoxygenic photosynthesis, aerobic and anaerobic respiration1.
When O2 tension is little, light energy can be harnessed by a photosynthetic electron transport chain that has similar features to those used by plants and other oxygen-evolving organisms (3). During photosynthetic growth, R. palustris is capable of use either carbon dioxide (CO2) or organic carbon sources.
Biotechnological potential
R. palustris is an excellent chassis because is useful in a wide variety of biotechnological applications because of its regulation systems that allow it to sense environmental conditions.
Why synthetic biology in Rhodopseudomonas palustris?
The Synthetic Biology application in R. palustris could help to harness its biotechnological
potential.
We want to investigate the functioning of 2 orthologous regulation systems that respond to
oxygen and light inspired in Rhodobacter sphaeroides regulatory systems.
Also we aim to generate a set of regulatory biobricks for Purple Non-Sulfur Photosynthetic
Bacteria, something that nowadays has not been reported.
References
1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) The Purple Phototrophic Bacteria. Springer;
200928. pp. 707–725.
2. Harwood et. al. (2004) Complete genome sequence of the metabolically versatile photosynthetic
bacterium Rhodopseudomonas palustris Nature Biotechnology Volume 2, Number 1, January 2004
3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J. Noguera D., Donohue T. (2011) iRsp1095: A genome-
scale reconstruction of the Rhodobacter sphaeroides metabolic network BMC Systems Biology 2011, 5:116
Rhodofactory 2012
