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<h1><em>Overview! </em></h1>
 +
<p>The purple non-sulfur photosynthetic bacteria (PNSB) belong to the alpha-proteobacteria group, because of their genetic regulatory systems
 +
which coordinate different metabolic states, these microorganisms are able to grow under a wide variety of environmental conditions (1).<br><br>
 +
 
 +
Specifically, our project aims to isolate two genetic control systems based on <em>Rhodobacter sphaeroides</em> photosynthesis cluster regulation. The first one is the
 +
oxygen dependant system PrrA/PrrB, when oxygen tension is high it remains inactive, and when the oxygen is low it activates gene expression
 +
(2). The second system is the light and oxygen mediated system AppA/PpsR that represses gene expression under aerobic conditions and allows
 +
transcription in the absence of oxygen and presence light (3).<br><br>
 +
 
 +
To achieve this goal we designed two genetic biobrick in which GFP expression is oxygen and light-dependent by the antirepression of PpsR and
 +
oxygen dependent by the activation of PrrA/B system. The lab work is accompanied by a computational model, which will provide a way of
 +
testing our knowledge of these systems.<br><br>
 +
 
 +
Once, we have characterized the functionality of these regulatory systems we aim to take advantage of <em>Rhodopseudomonas palustris’</em> metabolic versatility, and use
 +
this bacteria as a microbial factory, that could work for the production of metabolites with economic value products using CO2 as carbon source.<br><br>
 +
 
 +
We are planning to use the S04147 clostridial butanol production operon (University of Alberta iGEM Team 2007) to evaluate the synthesis of
 +
this biofuel, linking it to our control systems. This would provide an interesting way to produce butanol using CO2 as carbon source under
 +
anaerobic photosynthetic conditions.</p>
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      <p align="justify">Purple non-sulfur photosynthetic bacteria (PNSB) are metabolically versatile organisms that belong to the  alpha-proteobacteria. This microorganisms are able to grow  under a wide variety of environmental conditions, this is possible due to their sophisticated regulatory systems which coordinate metabolic changes. Our project aims to build two  genetic control systems based on <em>R. sphaeroides</em> photosynthesis  regulation <strong>(1).</strong></p>  
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      <p> The first one is the oxygen dependent system PrrBCA. It activates gene expression when the oxygen is low <strong>(2)</strong>. The second system is the light/oxygen mediated system that strongly represses gene expression under aerobic conditions and allows transcription in the absence of oxygen and light <strong>(3)</strong>. </p>
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<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
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        To achieve this goal we designed a genetic  circuit in which GFP expression is oxygen and light-dependent by the  antirepression of PpsR and oxygen dependent by the activation of PrrA/B system.  The lab work is accompanied by a computational model, which will provide a way  of testing our knowledge of these systems. </p>
+
<div id="sponsors">
-
      <p><br />
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  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div>
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        Once, we characterize these regulatory circuits we aim to exploit <em>R. palustris&rsquo; </em>metabolic versatility, and use it as a microbial factory,  that could work for the production  economic valuable products  using CO2 as carbon source.</p>
+
</div>
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      <p><br />
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<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div>
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        We are planning to use the S04147 (University of Alberta iGEM Team 2007) to  evaluate butanol production  controlled by our systems. This  would provide an interesting way to produce this biofuel using photosynthesis under anaerobic conditions. </p>
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    <div id="sponsors">
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      <p><br />
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      <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div>
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      <p><img src="https://static.igem.org/mediawiki/2012/c/cf/Over1.jpg" width="468" height="456" alt="Figure: General Scheme of the project" /></p>
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        <strong><font size="-2">Figure: </strong>General Scheme of the project<strong> </strong></p>
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      <ol>
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        <li>1.Hunter CN, Daldal  F, Thurnauer MC, Beatty JT: (2009) <strong>The  Purple Phototrophic Bacteria</strong>. Springer; 200928.<em> pp. 707–725.</em></li>
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        <li></li>
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        <li></li>
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        <li>2.Elsen S, Swem  LR, Swem DL, Bauer CE. (2004). <strong>RegB/RegA,  a highly conserved redoxresponding global two-component regulatory system</strong>. <em>Microbiol. Mol.  Biol. Rev. </em>68:263–79.</li>
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        <li> </li>
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        <li>3.Shinji Masuda2 and Carl E. Bauer (2002)<strong>  AppA Is a Blue Light  Photoreceptor that Antirepresses Photosynthesis Gene Expression in <em>Rhodobacter  sphaeroides </em></strong>Cell, Vol. 110,  613–623, September 6, 2002.</li>
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      <p>&nbsp;</p>
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Latest revision as of 03:30, 27 October 2012

Rho

Overview!

The purple non-sulfur photosynthetic bacteria (PNSB) belong to the alpha-proteobacteria group, because of their genetic regulatory systems which coordinate different metabolic states, these microorganisms are able to grow under a wide variety of environmental conditions (1).

Specifically, our project aims to isolate two genetic control systems based on Rhodobacter sphaeroides photosynthesis cluster regulation. The first one is the oxygen dependant system PrrA/PrrB, when oxygen tension is high it remains inactive, and when the oxygen is low it activates gene expression (2). The second system is the light and oxygen mediated system AppA/PpsR that represses gene expression under aerobic conditions and allows transcription in the absence of oxygen and presence light (3).

To achieve this goal we designed two genetic biobrick in which GFP expression is oxygen and light-dependent by the antirepression of PpsR and oxygen dependent by the activation of PrrA/B system. The lab work is accompanied by a computational model, which will provide a way of testing our knowledge of these systems.

Once, we have characterized the functionality of these regulatory systems we aim to take advantage of Rhodopseudomonas palustris’ metabolic versatility, and use this bacteria as a microbial factory, that could work for the production of metabolites with economic value products using CO2 as carbon source.

We are planning to use the S04147 clostridial butanol production operon (University of Alberta iGEM Team 2007) to evaluate the synthesis of this biofuel, linking it to our control systems. This would provide an interesting way to produce butanol using CO2 as carbon source under anaerobic photosynthetic conditions.

rodo01

 

Rhodofactory 2012

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