"
Team:NYMU-Taipei/ymin3.html
From 2012.igem.org
| (3 intermediate revisions not shown) | |||
| Line 63: | Line 63: | ||
<div id="ymi_header"> | <div id="ymi_header"> | ||
<div id="inner_header"> | <div id="inner_header"> | ||
| - | <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/ | + | <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/1/15/Ymi_header1.jpg" border="0"></a> |
</div> | </div> | ||
</div> | </div> | ||
| Line 164: | Line 164: | ||
<li><a title="Abstract" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li> | <li><a title="Abstract" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li> | ||
<li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li> | <li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li> | ||
| - | <li><a title=" | + | <li><a title="Experiments" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li> |
| - | <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results & References</a></li> | + | <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results & References</a></li><li><a title="Further Experiments after Asia Jamboree" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq5.html">Further Experiments after Asia Jamboree</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 189: | Line 189: | ||
<li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymic3.html">Methods & Materials</a></li> | <li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymic3.html">Methods & Materials</a></li> | ||
<li><a title="Results & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymic4.html">Results & Discussion</a></li> | <li><a title="Results & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymic4.html">Results & Discussion</a></li> | ||
| - | <li><a title=" | + | <li><a title="Conclusion & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymic5.html">Conclusion & References</a></li> |
</ul> | </ul> | ||
Latest revision as of 01:49, 27 October 2012
Part : BBa_K896004
NosZ-NorCB
In Pseudomonas aeruginosa PAO1, NorCB is found as two sub-units – nitric oxide reductase subunit C and B. As a matter of fact, these two subunits are adjacent genes. And thus we cloned them together to produce a functional reductase.
http://www.stanford.edu/group/collman/nor.htm
NosZ is the structure gene of nitrous reductase. Very few microorganisms process similar enzyme, and many of them are homologs according to NCBI database. Furthermore, it performs the last procedure of bio-denitrification – nitrous oxides to nitrogen – in our model.
Obviously, the reason we cloned NorCB and NosZ on the same plasmid is to connect the continuous denitrifying procedures together.
Part:BBa_K896006
NirN-NirS
Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.
Part:BBa_K896007
Nap( Nitrate->Nitrite reductase)
Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. For instance, even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.
-
Denitrifying Machine
-
Sulfur Oxide Terminator
-
Sulfide as Energy Generator
-
Cd+2 Collector
-
Becoming Venusian
