Team:Penn/Parts

From 2012.igem.org

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<b><div class="name" align="center">Parts Submitted</div></b><br>
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<b><div class="name">INPNC-MCS (Multiple Cloning Site): Winner of "Best BioBrick, Engineered" at Americas East</div></b>
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<h1>Parts Submitted</h1>
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A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p>
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<br>
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<h2>ClyA</h2>
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<b><div class="name">ClyA</div></b>
<p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a>  </p>
<p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a>  </p>
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<h2>His-ClyA</h2>
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<b><div class="name">His-ClyA</div></b>
<p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p>
<p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p>
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<h2>ClyA-His</h2>
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<b><div class="name">ClyA-His</div></b>
<p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p>
<p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p>
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<h2>INPNC</h2>
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<b><div class="name">INPNC</div></b>
<p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p>
<p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p>
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<h2>INPNC-HA</h2>
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<p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p>
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<b><div class="name">INPNC-HA</div></b>
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<h2>INPNC-MCS (Multiple Cloning Site)</h2>
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<p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811004"> BBa_K811004 </a></p>
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A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p>
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Latest revision as of 03:59, 27 October 2012

Penn 2012 iGEM Wiki

Image Map

Parts Submitted

INPNC-MCS (Multiple Cloning Site): Winner of "Best BioBrick, Engineered" at Americas East
 A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811005


ClyA

Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See BBa_K811000


His-ClyA

A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See BBa_K811001


ClyA-His

A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See BBa_K811002


INPNC

A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811003


INPNC-HA

A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811004


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