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| | {{:Utk knoxville calendar template}} | | {{:Utk knoxville calendar template}} |
| - | 9/21/12
| |
| - | Colony PCR Confirmed:
| |
| - | J63010 +Adh + mOrange + pIRES + GFP + cyc
| |
| - | J63010 +Adh + mOrange + pIRES2 + GFP + cyc
| |
| - | J63010 +Adh + mOrange + pSAP + GFP + cyc
| |
| | | | |
| - | 9/23/12
| + | 10/1/12<br> |
| - | Colony PCR Confirmed:
| + | Colonies finally appeared on plates. Colonies were picked into corresponding 200ug/ml G418 YPD plates. No colonies grew in culture.<br> |
| - | J63010 + Adh + Neo
| + | |
| - | J63010 +Adh + mOrange + YAP1 + GFP + cyc
| + | |
| - | J63010 +Adh + mOrange + URE2 + GFP + cyc
| + | |
| - | J63010 +Adh + mOrange + HAP4 + GFP + cyc
| + | |
| - | | + | |
| - | | + | |
| - | 9/24/12
| + | |
| - | Digestions:
| + | |
| - | J63010 + pIRES + GFP + cyc : SpeI and PstI
| + | |
| - | J63010 + pIRES2 + GFP + cyc : SpeI and PstI
| + | |
| - | J63010 + pSAP + GFP + cyc : SpeI and PstI
| + | |
| - | J63010 + Adh + Neo : XbaI and PstI
| + | |
| - | J63010 +Adh + mOrange + pIRES + GFP + cyc + Adh + Neo : EcoRI and PstI
| + | |
| - | J63010 + Adh + mOrange +pIRES2 + GFP + cyc + Adh + Neo: EcoRI and PstI
| + | |
| - | J63010 + Adh + mOrange +pSAP + GFP + cyc + Adh + Neo: EcoRI and PstI
| + | |
| - | J63010 + Adh + mOrange + cyc : SpeI and PstI
| + | |
| - | J63010 + Adh + GFP + cyc: SpeI and PstI
| + | |
| - | | + | |
| - | | + | |
| - | 9/25/12
| + | |
| - | Ligations performed to create constructs:
| + | |
| - | J63010 + Adh + mOrange + cyc + Adh + Neo
| + | |
| - | J63010 + Adh + GFP + cyc + Adh + Neo
| + | |
| - | J63010 + pIRES + GFP + cyc + Adh + Neo
| + | |
| - | J63010 + pIRES2 + GFP + cyc + Adh + Neo
| + | |
| - | J63010 + pSAP + GFP + cyc + Adh + Neo
| + | |
| - | | + | |
| - | 9/28/12
| + | |
| - | Colony PCR Confirmed:
| + | |
| - | J63010 + Adh + mOrange + cyc + Adh + Neo (AOC)
| + | |
| - | J63010 + Adh + GFP + cyc + Adh + Neo (AGC)
| + | |
| - | J63010 + pIRES + GFP + cyc + Adh + Neo (pIRES control)
| + | |
| - | J63010 + pIRES2 + GFP + cyc + Adh + Neo (pIRES2 control)
| + | |
| - | J63010 + pSAP + GFP + cyc + Adh + Neo (pSAP control)
| + | |
| - | | + | |
| - | BY4741 Yeast electroporated with:
| + | |
| - | AOC, AGC, pIRES control, pIRES2 control, pSAP control,pIRES complete, pIRES2 complete, pSAP complete.
| + | |
| - | Electroporations were plated onto 200ug/ml G418 YPD plates.
| + | |
| - | | + | |
| - | 10/1/12 | + | |
| - | Colonies finally appeared on plates. Colonies were picked into corresponding 200ug/ml G418 YPD plates. No colonies grew in culture. | + | |
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