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Utk knoxville august
From 2012.igem.org
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| - | 8.20.12 | + | 8.20.12 <br> |
| - | Digested PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc with EcoRI & PstI for confirmation. | + | Digested PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc with EcoRI & PstI for confirmation. <br> |
| - | Digested J63010 + Adh + mOrange with SpeI and PstI. Digested J63010 + pIRES + GFP + cyc with XbaI and PstI. | + | Digested J63010 + Adh + mOrange with SpeI and PstI. Digested J63010 + pIRES + GFP + cyc with XbaI and PstI. <br> |
| - | 8.21.12 | + | 8.21.12 <br> |
| - | Ran gel of check digestions for PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc. All three constructs were confirmed. | + | Ran gel of check digestions for PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc. All three constructs were confirmed. <br> |
| - | Ligated pIRES + GFP + cyc into J63010 + Adh + mOrange. Did 1:0, 1:10, and 1:30. | + | Ligated pIRES + GFP + cyc into J63010 + Adh + mOrange. Did 1:0, 1:10, and 1:30. <br> |
| - | Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc. | + | Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc. <br> |
| - | 8.26.12 | + | 8.26.12 <br> |
| - | Amplified GFP + Cyc. | + | Amplified GFP + Cyc. <br> |
| - | Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc. | + | Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc. <br> |
| - | 8.28.12 | + | 8.28.12 <br> |
| - | Digested : | + | Digested : <br> |
| - | J63010 + Adh with EcoRI and PstI to confirm construct. | + | J63010 + Adh with EcoRI and PstI to confirm construct. <br> |
| - | J63010 + Adh with SpeI and PstI. | + | J63010 + Adh with SpeI and PstI. <br> |
| - | PSB1C3+ KanR with XbaI and PstI. | + | PSB1C3+ KanR with XbaI and PstI. <br> |
| - | PSB1C3 + p150 with SpeI and PstI. | + | PSB1C3 + p150 with SpeI and PstI. <br> |
| - | Amplified GFP + Cyc with XbaI and PstI. | + | Amplified GFP + Cyc with XbaI and PstI. <br> |
Latest revision as of 03:07, 4 October 2012
