Team:RHIT/Outreach
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- | <img src="https://static.igem.org/mediawiki/igem.org/8/87/Banner_only2.png" width="100%"/> | + | <a href="https://2012.igem.org/Team:RHIT"><img src="https://static.igem.org/mediawiki/igem.org/8/87/Banner_only2.png" width="100%"/></a> |
<div id="logged_out"> | <div id="logged_out"> | ||
<a href="https://2012.igem.org/Main_Page">iGEM Home</a> | <a href="https://2012.igem.org/Main_Page">iGEM Home</a> | ||
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<div align="center"><img src="https://static.igem.org/mediawiki/igem.org/1/19/THCM_collab.png" width="90%"/></div><br /> | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/1/19/THCM_collab.png" width="90%"/></div><br /> | ||
<h4>Origins of the Project</h4> | <h4>Origins of the Project</h4> | ||
- | <p>The Terre Haute Children's Museum, founded in 1988, features many exhibits on topics ranging from archaeology to architecture. However, the Rose-Hulman team noticed that there are no exhibits on small-scale biology, specifically synthetic biology. Devon and Kristen then took on the project of creating an exhibit to introduce children to the concepts and methods of synthetic biology. The team felt that one of the best ways to get kids interested in synthetic biology was to show them lab equipment that they could touch and play with. The exhibit also gives kids a chance to explore designing DNA sequences. In this way, they can experience a process that synthetic biologists go through.</p> | + | <p>The <a href="http://www.terrehautechildrensmuseum.com/">Terre Haute Children's Museum</a>, founded in 1988, features many exhibits on topics ranging from archaeology to architecture. However, the Rose-Hulman team noticed that there are no exhibits on small-scale biology, specifically synthetic biology. Devon and Kristen then took on the project of creating an exhibit to introduce children to the concepts and methods of synthetic biology. The team felt that one of the best ways to get kids interested in synthetic biology was to show them lab equipment that they could touch and play with. The exhibit also gives kids a chance to explore designing DNA sequences. In this way, they can experience a process that synthetic biologists go through.</p> |
<h4>Exhibit Components</h4> | <h4>Exhibit Components</h4> | ||
- | <p>The Design Table, pictured below, introduces children to the idea of putting together DNA sequences. The instructions on the table ask them to think of ways they would use synthetic biology if they could make cells "do anything." The team hopes that this will inspire them to use creativity to explore the possibilities of synthetic biology. Colored magnetic shapes are used to represent DNA. Children can select from different colors, each with a different purpose - the four DNA nucleotide bases, A, T, G, and C, as well as Promoters and Terminators - and align them on the board to create their sequence. They also have the option of putting together various sequences found on cards on the table or create the matching sequence to given DNA sequences. This gives more uncertain children the chance to understand the process. Examples of these cards are seen below.</p><br /> | + | <p>The Design Table, pictured below, introduces children to the idea of putting together DNA sequences. |
+ | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/0/05/Design_table.png" width=55%></div><br /> | ||
+ | The instructions on the table ask them to think of ways they would use synthetic biology if they could make cells "do anything." The team hopes that this will inspire them to use creativity to explore the possibilities of synthetic biology. Colored magnetic shapes are used to represent DNA. Children can select from different colors, each with a different purpose - the four DNA nucleotide bases, A, T, G, and C, as well as Promoters and Terminators - and align them on the board to create their sequence. They also have the option of putting together various sequences found on cards on the table or create the matching sequence to given DNA sequences. This gives more uncertain children the chance to understand the process. Examples of these cards are seen below.</p><br /> | ||
<img src="https://static.igem.org/mediawiki/igem.org/4/46/Card1ex.png" width="100%"/><br /> | <img src="https://static.igem.org/mediawiki/igem.org/4/46/Card1ex.png" width="100%"/><br /> | ||
<p> After creating their sequence, the children move on to the Lab Bench, where they explore four different pieces of lab equipment. | <p> After creating their sequence, the children move on to the Lab Bench, where they explore four different pieces of lab equipment. | ||
- | The four stations are:<br /> | + | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/8/8f/Lab_bench.png" width=55%></div><br /> |
+ | <div align="center">The four stations are:<br /> | ||
<li>Microscope</li> | <li>Microscope</li> | ||
<li>Centrifuge</li> | <li>Centrifuge</li> | ||
<li>Pipetting</li> | <li>Pipetting</li> | ||
- | <li>Media Plates</li> | + | <li>Media Plates</li></div> |
Each station provides a description of the equipment as well as a procedure for the children to follow in order to gain experience with and understanding of the technology. Questions guide the children along the way and encourage them to think about the purpose or design of each piece of equipment. | Each station provides a description of the equipment as well as a procedure for the children to follow in order to gain experience with and understanding of the technology. Questions guide the children along the way and encourage them to think about the purpose or design of each piece of equipment. | ||
</p> | </p> | ||
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<div class="rhit-grnSheet" id="rhit-grnSheet"> | <div class="rhit-grnSheet" id="rhit-grnSheet"> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2012/8/86/Collab_logo.png" width="60%" /></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/86/Collab_logo.png" width="60%" /></div> | ||
+ | <h4>Introduction</h4><br /> | ||
<p>Throughout the summer, Rose-Hulman iGEM has been collaborating with <a href="https://2012.igem.org/Team:NTNU_Trondheim">NTNU Trondheim</a>. The teams found each other by luck when a speaker came to Rose-Hulman to meet with students participating in a math REU and talk about network modeling. This speaker was Eivind Almaas, NTNU’s iGEM main advisor. | <p>Throughout the summer, Rose-Hulman iGEM has been collaborating with <a href="https://2012.igem.org/Team:NTNU_Trondheim">NTNU Trondheim</a>. The teams found each other by luck when a speaker came to Rose-Hulman to meet with students participating in a math REU and talk about network modeling. This speaker was Eivind Almaas, NTNU’s iGEM main advisor. | ||
After touching base with our lead advisor, Dr. Anthony, Dr. Almaas provided us with great perspective about being a starting iGEM team because his team was in the same position last year. | After touching base with our lead advisor, Dr. Anthony, Dr. Almaas provided us with great perspective about being a starting iGEM team because his team was in the same position last year. | ||
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<p>On 7/23/2012, the two teams met for the first time via video conferencing to talk about their projects and to offer advice to the other team. | <p>On 7/23/2012, the two teams met for the first time via video conferencing to talk about their projects and to offer advice to the other team. | ||
In efforts to help each other out, RHIT agreed to help to characterize their lactate promote. In exchange, the NTNU team agreed to help critique our Mathematical Model and produce a stochastic model. | In efforts to help each other out, RHIT agreed to help to characterize their lactate promote. In exchange, the NTNU team agreed to help critique our Mathematical Model and produce a stochastic model. | ||
- | We hope that this newly found friendship will continue throughout the years and that we will be lucky enough to meet the whole team in person this November.</p> | + | We hope that this newly found friendship will continue throughout the years and that we will be lucky enough to meet the whole team in person this November.</p><br /><br /> |
- | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/b/bd/NorwayProfPic.png" width=45%" / | + | <h4>Promoter Characterization</h4><br /> |
- | <h4> | + | <p>Our NTNU colleagues requested help characterizing the Lld promoter they cloned from E. coli. strain K12. We received the promoter as a 373 bp EcoRI-SpeI fragment cloned into pSB1C3. The fragment was sequenced in both directions using primers VF2 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100">BBa_G00100</a>) and VR (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101">BBa_G00101</a>). The sequence obtained matched that which was expected and is given in the Registry (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K822000">BBa_K822000</a>).</p><br /><br /> |
+ | <p>We then used standard BioBrick construction methods to clone the EcoRI-SpeI promoter fragment into the EcoRI-XbaI sites upstream of the cyan fluorescent protein cloned into pSB1A3 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_E0422">BBa_E0422</a>). The putative construct was verified by restriction digest analysis and sequenced in both directions using primers VF2 and VR to validate the expected sequence.</p><br/ ><br /> | ||
+ | <p>NEB5alpha cells harboring the reporter construct were spread on LB plates supplemented with 100 mM lithium lactate and incubated at 37 C. The plates were monitored over a 36 hour period and showed no fluorescence. Similar experiments using liquid media and a spectrofluorometer for measurement also indicated no fluorescence.</p><br /><br /> | ||
+ | <h4>Stochastic Model</h4><br /> | ||
+ | <p>The NTNU team created a stochastic model about part of our system. For a more in-depth description, please visit our <a href="https://2012.igem.org/Team:RHIT/Modeling?tab=page3">Modeling page</a>.<br /><br /> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/b/bd/NorwayProfPic.png" width=45%" /> | ||
+ | <h4>Team's Advisors: Dr. Ric Anthony (left) and Dr. Eivind Almaas (right).</h4><br /><br /></div> | ||
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 03:55, 4 October 2012