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User:DrJones1935/7 June 2012
From 2012.igem.org
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====I. Inoculate Wild Type (WT) ''B. subtilis'' culture from Altman Lab in Nutrient Broth (Lim)==== | ====I. Inoculate Wild Type (WT) ''B. subtilis'' culture from Altman Lab in Nutrient Broth (Lim)==== | ||
Latest revision as of 22:59, 3 October 2012
I. Inoculate Wild Type (WT) B. subtilis culture from Altman Lab in Nutrient Broth (Lim)
- Add ~12.5 mL of Nutrient broth to sterile 125 mL E-flask, re-cover with tin foil
- Inoculate 1 colony from pre-existing plate culture with inoculating loop to flask (11:05 AM)
- Grow at 37 oC with shaking overnight
