Team:UT Dallas/pop3 o results

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<h2 class='title' style='font-size: 120%;'>Single Population Toggle Switch Design</h2>
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<h2 class='title' style='font-size: 120%;'>Three Populations Cascade Results</h2>
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Phase 1: Promotion<br><br>
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Phase 1: Promotion
    
    
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To test our three basic populations two test tubes containing 2mL of ampicillin-containing media were mixed with 400 microliters of each of the populations’ DNA.  However, only one of the test tubes were induced by 1.5 mL of pseudo-population 3. In this way the non-induced test tube acted as a negative control.<br>
To test our three basic populations two test tubes containing 2mL of ampicillin-containing media were mixed with 400 microliters of each of the populations’ DNA.  However, only one of the test tubes were induced by 1.5 mL of pseudo-population 3. In this way the non-induced test tube acted as a negative control.<br>
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     Through our testing we have shown that our three Basic Populations successfully work together. Basic Populations 1, 2 and 3 all exhibited a significant increase in fluorescence when induced, compared to when it was not induced<br><br>
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     Through our testing we have shown that our three Basic Populations successfully work together. Populations 1, 2 and 3 all exhibited a significant increase in fluorescence when induced, compared to when it was not induced<br><br>
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Phase 2: Promotion and Repression<br><br>
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Unfortunately, due to time restraints, we were not able to finish reconstruction of the three populations that would allow them to exhibit repression of their fluorescence by the quorum sensing molecules. However, we look forward to continuing and finishing our work in the future.
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<center>
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<img width="750" src="https://static.igem.org/mediawiki/2012/f/f9/Pop1%2B2%2B3.png">
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</center>

Latest revision as of 02:28, 4 October 2012

Three Populations Cascade Results

Phase 1: Promotion


To test our three basic populations two test tubes containing 2mL of ampicillin-containing media were mixed with 400 microliters of each of the populations’ DNA. However, only one of the test tubes were induced by 1.5 mL of pseudo-population 3. In this way the non-induced test tube acted as a negative control.

   Through our testing we have shown that our three Basic Populations successfully work together. Populations 1, 2 and 3 all exhibited a significant increase in fluorescence when induced, compared to when it was not induced

<img width="750" src="Pop1%2B2%2B3.png">

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