Team:UCSF/Protocols2

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<h3red>Violacein Production Protocols</h3red> <p><br>
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<h3red>Violacein Production Protocols</h3red> <p>
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<li>Growing Cultures Anaerobically</li>
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<li>Extracting Pigments</li>
<li>Extracting Pigments</li>
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<regulartext> To extract pigments, samples were taken from each culture and centrifuged to pellet the cells. For most experiments a 2ml culture was sufficient and this cell pellet was resuspended in the desired solvent. As seen in our data page, several solvents were tested but our final analysis was done with an ethanol extraction. After addition of the solvent (~300ul) the sample was vortexed and then centrifuged again to remove cell precipitate. The resulting organic phase was used for analysis.
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<li>Growing in M9</li>
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<li>Growing Monocultures</li>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/monoprotocol.pdf"> Monoculture of Auxotroph Strains</a> to determine growth rate in presence of amino acids<br></li>
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Coculturing%20of%20Auxotrophs.pdf"> Co-culturing of Auxotroph Strains</a> <br></li></ul>
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Coculturing%20of%20Auxotrophs.pdf"> Co-culturing of Auxotroph Strains</a> <br></li></ul>
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<h3red>Toxin/Antitoxin System Protocols</h3red><br>
<h3red>Toxin/Antitoxin System Protocols</h3red><br>
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<li>Inducing for Protein Production</li>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Toxin-Antitoxin%20Expression%20Protocol-3.pdf"> Inducing for Protein Production</a></li>
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<li>Supernatent Switch Experiment</li>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Toxin-Antitox%20Exchange%20Protocol.pdf"> Toxin-Antitoxin Exchange Protocol</a></li>
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Latest revision as of 03:25, 4 October 2012

Violacein Production Protocols

  • Extracting Pigments
    • To extract pigments, samples were taken from each culture and centrifuged to pellet the cells. For most experiments a 2ml culture was sufficient and this cell pellet was resuspended in the desired solvent. As seen in our data page, several solvents were tested but our final analysis was done with an ethanol extraction. After addition of the solvent (~300ul) the sample was vortexed and then centrifuged again to remove cell precipitate. The resulting organic phase was used for analysis.


Auxotroph System Protocols


Toxin/Antitoxin System Protocols

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