Team:HokkaidoU Japan/Notebook

From 2012.igem.org

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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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{{Team:HokkaidoU_Japan/header}}
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!align="center"|[[Team:HokkaidoU_Japan|Home]]
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{{Team:HokkaidoU_Japan/nav.notebook}}
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!align="center"|[[Team:HokkaidoU_Japan/Team|Team]]
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<div id="hokkaidou-column-main">
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=HokkaidoU_Japan Official Team Profile]
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<!-- DO NOT EDIT OVER THIS LINE @iTakeshi -->
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!align="center"|[[Team:HokkaidoU_Japan/Project|Project]]
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!align="center"|[[Team:HokkaidoU_Japan/Parts|Parts Submitted to the Registry]]
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<style type="text/css">
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!align="center"|[[Team:HokkaidoU_Japan/Modeling|Modeling]]
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    .hokkaidou-notebook-index {
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!align="center"|[[Team:HokkaidoU_Japan/Notebook|Notebook]]
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!align="center"|[[Team:HokkaidoU_Japan/Safety|Safety]]
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!align="center"|[[Team:HokkaidoU_Japan/Attributions|Attributions]]
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== Hello ==
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We are team HokkaidoU Japan! Today we learn and start to edit wiki.
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(>ω<) <br>
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Dear Mr.Ortiz, I saw the help page which you edited.<br>
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hola!<br>
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    .hokkaidou-notebook-index h5 {
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=July=
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==4th==
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Transformation
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*Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
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    .hokkaidou-notebook-index h5 {
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**Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43)
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==5th==
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    .hokkaidou-notebook-index:hover p {
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*Transformation of K346007(Ag43)in DH5α.
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**Cultivation on LBC.
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Single colony isolation
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*Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
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**BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
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==6th==
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Liquid culture
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*Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
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    .hokkaidou-notebook-index:hover {
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**LB : 1ml is for glycerol stocks and 2ml is for mini-prep
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Single colony isolation
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    #hokkaidou-notebook-bootcamp {
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*Single colony isolation of K346007(Ag43).
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    #hokkaidou-notebook-protocols {
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</style>
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<h2>Notebook</h2>
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<div class="hokkaidou-section">
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<a href="/Team:HokkaidoU_Japan/Notebook/Spring_Boot_Camp" class="hokkaidou-notebook-index" id="hokkaidou-notebook-bootcamp">
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  <h5>Boot Camp</h5>
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  <p>Be my apprentice.</p>
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</a>
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<a href="/Team:HokkaidoU_Japan/Notebook/Lab_Diary" class="hokkaidou-notebook-index" id="hokkaidou-notebook-diary">
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  <h5>Lab Diary</h5>
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  <p>That's one small step for you, one giant leap for us. </p>
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</a>
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<a href="/Team:HokkaidoU_Japan/Notebook/overall_protocols" class="hokkaidou-notebook-index" id="hokkaidou-notebook-protocols">
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  <h5>Protocols</h5>
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  <p>Bible of our lab.</p>
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</a>
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</div>
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</html>
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<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi -->
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</div>
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<br style="line-height: 0; clear: both;" />
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{{Team:HokkaidoU_Japan/footer}}

Latest revision as of 00:52, 27 September 2012

Notebook