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| - | {{:Team:Michigan/Template:Michigan Header}} | + | {{:Team:Michigan/Template:New}} |
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| - | [[Team:Michigan/Notebook/Protocols]]
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| | {| style="color:#ffffff;background-color:#929487;" cellpadding="3" cellspacing="1" border="0" width="100%" align="center" | | {| style="color:#ffffff;background-color:#929487;" cellpadding="3" cellspacing="1" border="0" width="100%" align="center" |
| | !align="center"|[[Team:Michigan|Home]] | | !align="center"|[[Team:Michigan|Home]] |
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| | !align="center"|[[Team:Michigan/Attributions|Attributions]] | | !align="center"|[[Team:Michigan/Attributions|Attributions]] |
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| - | <!-- *** Here is where you should enter your lab notebook! Remember to name and date your entries. Use the icons on the top left corner to embed image, bold, italicize, and so forth. For technical support, email huetone@gmail.com or ask Candy. *** -->
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| | <html> | | <html> |
| - | <h2>Modify HbiF into a Biobrick compatible format </h2> | + | <div class="body"> |
| - | <p>HbiF and LVA degradation tag were ordered from IDT with the following specifications.</p> | + | <div id="basic"> |
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| - | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">EcoRI</span></p> | + | <!-- What is this? |
| - | <p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p> | + | <div class="locator"> |
| - | <p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p> | + | <p>< a href=" /Team: Michigan/Project"> Project</ a> > < a href=" /Team: Michigan/Noteboook"> Notebook</ a></p> |
| - | <p> <span style="background-color:#A9C9A4;font-family:Courier, monospace;">PstI</span></p> | + | </div> |
| - | <p> <span style="background-color:#86C67C;font-family:Courier, monospace;">AgeI</span></p>
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| - | <p> <span style="background-color:#CD96CD;font-family:Courier, monospace;">NgoMIV</span></p>
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| - | <p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :</p>
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| - | <p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p>
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| - | <p><br/>No extra amino acids are created by this prefix, in comparison to the standard RFC25 prefix, which creates a serine and glycine. The suffix proceeds as follows:</p>
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| - | <p style="font-family:Courier, monospace;"><span style="background-color:#86C67C;">ACCGGT</span> TAAT <span style="background-color:#C8E0DB">ACTAGT</span> A GCGGCCG <span style="background-color:#A9C9A4">CTGCAG</span> </p>
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| | + | <!-- *** Here is where you should enter your lab notebook! Remember to name and date your entries. Use the icons on the top left corner to embed image, bold, italicize, and so forth. For technical support, email huetone@gmail.com or ask Candy. *** --> |
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| | + | <h2>Cloning Team Notebook</h2> |
| | + | <iframe width=100% height=800; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe> |
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| - | <h2>Prepare FimE for protein expression</h2>
| + | </div> |
| - | <iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe>
| + | </div> |
| - | <h2>Team Reports</h2>
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| - | <iframe src="https://docs.google.com/presentation/embed?id=17czITWHCoyzbzdYCvXWmwpDbl31jJyhgNmb32IXX3aY&start=false&loop=false&delayms=8000" frameborder="0" width=60% height=400 allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe>
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| | </html> | | </html> |
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