Team:Duke/Attributions

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<li><a href="https://2012.igem.org/Team:Duke/Notebook" accesskey="5"  
<li><a href="https://2012.igem.org/Team:Duke/Notebook" accesskey="5"  
title="">Lab Notebook</a></li>
title="">Lab Notebook</a></li>
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<li><a href="https://2012.igem.org/Team:Duke/Attributions" accesskey="5"  
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<li><a href="https://2012.igem.org/Team:Duke/Sponsors" accesskey="5"  
title="">Sponsors</a></li>
title="">Sponsors</a></li>
                 <li><a href="https://2012.igem.org/Team:Duke/Human Practices" accesskey="6"  
                 <li><a href="https://2012.igem.org/Team:Duke/Human Practices" accesskey="6"  
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<h2 class="title"><a href="#">Welcome to the Duke iGEM Wiki</a></h2>
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<h2 class="title"><a href="#">Attributions</a></h2>
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<h2>Duke iGEM 2012</h2>
 
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<p>Our team is fully aware of it's disadvantages, being smaller than the rest, and holding less funding than the competition. However, these notions motivate each individual on the team rather than discourage us. We know that we will get out of this project, exactly what we put into it. Understanding this notion is why it is not unusual to find our team working 12 hour shifts daily. We are inspired and determined to contribute to the scientific community in a substantial way, utilizing our resources, and setting new standards.</p>
 
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<h2>Optogenetics: The <u>hot</u> topic </h2>
 
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Optogenetics is the combination of genetic and optical methods to control specific events in targeted cell. In 2010, optogenetics was chosen as the Method of the Year across all fields of science and engineering by the interdisciplinary research journal Nature Methods. At the same time, optogenetics was highlighted in the article on "Breakthroughs of the Decade" in the scientific research journal Science Breakthrough of the Decade.
 
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<h2>Useful Links</h2>
 
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<li><a href="http://www.genome.duke.edu/">Duke University IGSP</a></li>
 
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<li><a href="http://www.bowmanbrockman.org/ncssm/">Bowman Brockman</a></li>
 
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<li><a href="http://www.geneoracle.com/">Gene Oracle</a></li>
 
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<li><a href="http://www.synberc.org/">SynBERC</a></li>
 
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<li><a href="http://pubs.acs.org/journal/asbcd6">ACS Synethetic Biology</a></li>
 
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Although some specific tasks are listed for each lab member, by the end of the summer each lab member was equally proficient in all lab techniques although each member had their preferences which are outlined below.
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==Param Sidhu==
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Param was one of the main driving forces behind lab work. He spent large amounts of time planning the daily schedule, figuring out which restriction digests should be completed, mini-prepping DNA, and cloning plasmids. He also learned to operate the flow cytometer and analyze flow cytometric data. He continued working on the project after the summer ended and the school year began.
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==Morgan Howell==
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Morgan was the other main driving force behind lab work. His duties included maintaining the lab notebook, helped to design the wiki, growing yeast, growing bacteria, transforming yeast and bacteria, running gels, and programming the FCAnalyze program which significantly expedited the analysis of flow cytometric data. He continued working on the project after the summer ended and the school year began.
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==Peter Fan==
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Peter helped to develop the compuational Tinker Cell Model of the Optogenetic system and also contributed to the lab work through running gels and digests, preparing yeast for flow cytometry, and transforming bacteria. He also participated in more menial tasks such as labeling tubes and making aliquots. Peter also helped implement many of the human practice approaches and worked to maintain the wiki. Regretfully, Peter was unable to continue coming to lab after the school year began but continued helping the team through devising the wiki, human practice approaches, and working on the tinker cell model.
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==Mentors and Lab==
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The team is indebted to Buchler Lab and Gersbach Lab for their mentorship. The Principal Investigators and Lab Members served largely as mentors and supervisors. Lab Members trained the team members in a variety of lab techniques and helped provide pointers and tips on ways to enhance DNA yields and document their work to prevent errors.
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==Tucker Lab of UC Denver==
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Our work built off of existing work by Chandra Tucker and her lab at UC Denver. The Tucker Lab designed and built the two-hybrid proteins and plasmids that we used in our work.
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See the following methods paper for more information about construction and use of these constructs in yeast:
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Each team must clearly attribute work done by the team on this page. They must distinguish work done by the team from work done by others, including the host labs, advisors, instructors, graduate students, and postgraduate masters students.
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Hughes, R.M., Bolger, S., Tapadia, H., and Tucker, C.L. (2012) Light-mediated control of DNA transcription in yeast. Methods. epub Aug 15

Latest revision as of 21:27, 15 October 2012

Attributions

Although some specific tasks are listed for each lab member, by the end of the summer each lab member was equally proficient in all lab techniques although each member had their preferences which are outlined below.

Contents

Param Sidhu

Param was one of the main driving forces behind lab work. He spent large amounts of time planning the daily schedule, figuring out which restriction digests should be completed, mini-prepping DNA, and cloning plasmids. He also learned to operate the flow cytometer and analyze flow cytometric data. He continued working on the project after the summer ended and the school year began.

Morgan Howell

Morgan was the other main driving force behind lab work. His duties included maintaining the lab notebook, helped to design the wiki, growing yeast, growing bacteria, transforming yeast and bacteria, running gels, and programming the FCAnalyze program which significantly expedited the analysis of flow cytometric data. He continued working on the project after the summer ended and the school year began.

Peter Fan

Peter helped to develop the compuational Tinker Cell Model of the Optogenetic system and also contributed to the lab work through running gels and digests, preparing yeast for flow cytometry, and transforming bacteria. He also participated in more menial tasks such as labeling tubes and making aliquots. Peter also helped implement many of the human practice approaches and worked to maintain the wiki. Regretfully, Peter was unable to continue coming to lab after the school year began but continued helping the team through devising the wiki, human practice approaches, and working on the tinker cell model.

Mentors and Lab

The team is indebted to Buchler Lab and Gersbach Lab for their mentorship. The Principal Investigators and Lab Members served largely as mentors and supervisors. Lab Members trained the team members in a variety of lab techniques and helped provide pointers and tips on ways to enhance DNA yields and document their work to prevent errors.

Tucker Lab of UC Denver

Our work built off of existing work by Chandra Tucker and her lab at UC Denver. The Tucker Lab designed and built the two-hybrid proteins and plasmids that we used in our work. See the following methods paper for more information about construction and use of these constructs in yeast:

Hughes, R.M., Bolger, S., Tapadia, H., and Tucker, C.L. (2012) Light-mediated control of DNA transcription in yeast. Methods. epub Aug 15

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