Team:Cornell/Notebook/Nah operon/June24

From 2012.igem.org

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[[Team:Cornell/Notebook/Nah_operon|Back to nah operon week view]]
[[Team:Cornell/Notebook/Nah_operon|Back to nah operon week view]]
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===June 24th-30th===
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=June 24th-30th=
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====June 27th, Wednesday====
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==June 27th, Wednesday==
[[Image:2012_627_RafDylanGelMaking.jpg|thumb|right|Rafael and Dylan making an agarose gel.]]
[[Image:2012_627_RafDylanGelMaking.jpg|thumb|right|Rafael and Dylan making an agarose gel.]]
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*Ran digest of gibson-assembled nah operon with [[gel electrophoresis]]
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*Ran digest of gibson-assembled nah operon with [[Team:Cornell/Notebook/Gel_electrophoresis|gel electrophoresis]]
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====June 28th, Thursday====
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==June 28th, Thursday==

Latest revision as of 21:52, 3 July 2012

Home Team Project Parts Modeling Notebook Protocols Safety Attributions

Contents

The purpose of this subproject is to make the nah operon biobrick compatible for the use of future iGEM teams. The nah operon codes for a pathway that converts naphthalene into salicylate. In our system, this will allow naphthalene to be detected by the salicylate reporter. There are three standard cut sites internal to the operon which we are removing - PstI, and two NotI sites. This biobrick compatible piece could be useful to future teams in projects that involve bioremediation or detection of naphthalene. Back to nah operon week view

June 24th-30th

June 27th, Wednesday

Rafael and Dylan making an agarose gel.

==June 28th, Thursday==