From 2012.igem.org
(Difference between revisions)
|
|
| (4 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | ===June 10th-16th===
| |
| - | ====June 13th, Wednesday====
| |
| - | *Set up [[PCR]] for four fragments of the nah operon as well as the pBMT-1 backbone, using primers designed to mutate cut-sites concurrently with Gibson Assembly. Also set up a PCR for the entire nah operon. Due to length of the fragments, a longer extension time was chosen.
| |
| - | *If Gibson Assembly fails, but we are able to PCR the entire 10kb nah operon using Phusion polymerase, an alternate method of mutation using Phusion will be pursued.
| |
| - | **''Work done by: Caleb, Claire, Dylan, Spencer, Steven, and Swati''
| |
| | | | |
| - | ====June 14th, Thursday====
| |
| - | *PCR only amplified nah1, nah3, and nah4 - longer fragments (nah2, the full nah operon, and pBMT-1) were not identified on the PCR product gel. Set up another [[PCR]] with shorter extension time.
| |
| - |
| |
| - | ====June 15th, Friday====
| |
| - | *PCR of four out of five products visible on gel. Set up PCR of pBMT-1, final fragment required for Gibson Assembly of the nah operon.
| |
Latest revision as of 19:49, 19 July 2012
"
