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<h1><em>Chassis!</em></h1>
 +
            </br>
 +
<p><em>Rhodopseudomonas palustris</em> has an extraordinary metabolic versatility; this microorganism is able
 +
to grow in a wide variety of environmental conditions. <em>R palustris</em> obtain energy by different
 +
mechanism including anoxygenic photosynthesis, aerobic and anaerobic respiration<sup>1.</sup></p>
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2012/d/d7/Cha01.gif" alt="cha01" width="324" height="363"></div>
 +
<br>
 +
<p>When O2 tension is little, light energy can be harnessed by a photosynthetic electron transport
 +
chain that has similar features to those used by plants and other oxygen-evolving organisms (3).
 +
During photosynthetic growth, <em>R. palustris</em> is capable of use either carbon dioxide (CO2) or organic
 +
carbon sources.</p>
 +
<p id="text2">Biotechnological potential</p>
 +
<p><em>R. palustris</em> is an excellent chassis because is useful in a wide variety of biotechnological
 +
applications because of its regulation systems that allow it to sense environmental conditions.</p>
 +
    <img src="https://static.igem.org/mediawiki/2012/6/65/Cha02.gif" alt="cha02" width="557" height="376"><br>
 +
<p id="text2"><br>Why synthetic biology in <em>Rhodopseudomonas palustris</em>?
 +
</p>
 +
<p>The Synthetic Biology application in <em>R. palustris</em> could help to harness its biotechnological
 +
potential.<br>
 +
 
 +
We want to investigate the functioning of 2 orthologous regulation systems that respond to
 +
oxygen and light inspired in <em>Rhodobacter sphaeroides</em> regulatory systems.<br>
 +
 
 +
Also we aim to generate a set of regulatory biobricks for Purple Non-Sulfur Photosynthetic
 +
Bacteria, something that nowadays has not been reported.</p>
 +
<div align="center"><br>
 +
<div style="clear: both;">&nbsp;<img src="https://static.igem.org/mediawiki/2012/c/c0/Cha03.png" alt="cha03" width="555" height="419"></div>
    
    
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<div id="bodys_r1_c3">
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<hr>
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      <img src="https://static.igem.org/mediawiki/2012/4/4e/Chassis.fw.png" width="500" height="92" /> </div>
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<p id="refe">References<br>1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) <strong>The Purple Phototrophic Bacteria</strong>. Springer;
-
<div class="clearFloat"></div>
+
200928. pp. 707–725. <br>
-
<div id="bodys_r2_c2"></div>
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<div id="bodys_r2_c3">
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2. Harwood et. al. (2004) <strong>Complete genome sequence of the metabolically versatile photosynthetic
-
    <div class="Text">
+
bacterium <em>Rhodopseudomonas palustris</em></strong> Nature Biotechnology Volume 2, Number 1, January 2004<br>
-
   
+
 
-
      <p><em>Rhodopseudomonas palustris</em> has an extraordinary metabolic versatility; this organism can grow in a wide variety of environmental conditions. <em>R palustris</em> obtain energy by different  mechanism including <strong>anoxygenic</strong> <strong>photosynthesis, aerobic and anaerobic  respiration</strong>. </p>
+
3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J. Noguera D., Donohue T. (2011) <strong>iRsp1095: A genome-
-
      <p>&nbsp;</p>
+
scale reconstruction of the <em>Rhodobacter sphaeroides</em> metabolic network</strong> BMC Systems Biology 2011, 5:116
-
      <p><img src="https://static.igem.org/mediawiki/2012/a/af/Cha.jpg" width="640" height="266" /></p>
+
-
      <p><strong>Figure</strong> Metabolic versatility or R. palustris (Harwood et. al. (2004))</p>
+
-
      <p><br />
+
-
        When O2 is  absent or limiting, light energy can be harnessed by a photosynthetic electron  transport chain that has features similar to those used by plants and other  oxygen-evolving phototrophs <strong>(3)</strong>. During photosynthetic growth, <em>R. palustris</em> is capable of autotrophic  or heterotrophic growth using either carbon dioxide (CO2) or organic carbon  sources. <br />
+
-
        <strong>Biotechnological  potential</strong><br />
+
-
        <em>R. palustris </em>is an excellent candidate for use in a wide  variety of biotechnological applications because of a wide range of  regulation systems that allow it to sense environmental conditions.</p>
+
</p>
</p>
-
    <p><img src="https://static.igem.org/mediawiki/2012/c/cb/Cha2.jpg" width="640" height="474" /></p>
+
  </div>
-
    <p><strong>Why synthetic biology in <em>R. palustris</em>?</strong></p>
+
<div id="sidebar">
-
    <p>The application of Synthetic Biology in R. palustris  could help to exploit the biotechnological potential of this organism. We want  to explore the functioning of 2 orthologous regulation systems that respond to  oxygen and light inspired in <em>Rhodobacter  sphaeroides </em>regulatory systems.</p>
+
  <div id="updates" class="orangebox">
-
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+
<h2>Rhodofactory</h2>
-
    <p>1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT:  (2009) <strong>The Purple Phototrophic Bacteria</strong>. Springer; 200928.<em> pp. 707–725.</em> <br />
+
<ul>
-
      2.  Harwood  et. al. (2004) <strong>Complete genome sequence of the metabolically versatile  photosynthetic bacterium <em>Rhodopseudomonas palustris</em></strong>, Nature Biotechnology Volume 2, Number 1,  January 2004<br />
+
<li><a href="Overview.htm" target="_parent">Overview</a></li>
-
      3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J.  Noguera D., Donohue T. (2011)<strong> iRsp1095:  A genome-scale reconstruction of theRhodobacter sphaeroides metabolic network </strong>BMC  Systems Biology 2011, 5:116 </p>
+
<li><a href="Light_Response.htm">Light and Oxygen</a></li>
 +
<li><a href="Oxigen_Response.htm">Oxygen Response</a></li>
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<li><a href="Chassis.htm">Chassis</a></li>
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Latest revision as of 15:03, 26 October 2012

Rho

Chassis!


Rhodopseudomonas palustris has an extraordinary metabolic versatility; this microorganism is able to grow in a wide variety of environmental conditions. R palustris obtain energy by different mechanism including anoxygenic photosynthesis, aerobic and anaerobic respiration1.

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When O2 tension is little, light energy can be harnessed by a photosynthetic electron transport chain that has similar features to those used by plants and other oxygen-evolving organisms (3). During photosynthetic growth, R. palustris is capable of use either carbon dioxide (CO2) or organic carbon sources.

Biotechnological potential

R. palustris is an excellent chassis because is useful in a wide variety of biotechnological applications because of its regulation systems that allow it to sense environmental conditions.

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Why synthetic biology in Rhodopseudomonas palustris?

The Synthetic Biology application in R. palustris could help to harness its biotechnological potential.
We want to investigate the functioning of 2 orthologous regulation systems that respond to oxygen and light inspired in Rhodobacter sphaeroides regulatory systems.
Also we aim to generate a set of regulatory biobricks for Purple Non-Sulfur Photosynthetic Bacteria, something that nowadays has not been reported.


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References
1. Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) The Purple Phototrophic Bacteria. Springer; 200928. pp. 707–725.
2. Harwood et. al. (2004) Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris Nature Biotechnology Volume 2, Number 1, January 2004
3. Imam S., Yilmaz S., Sohmen Y, Gorzalski A., Reed J. Noguera D., Donohue T. (2011) iRsp1095: A genome- scale reconstruction of the Rhodobacter sphaeroides metabolic network BMC Systems Biology 2011, 5:116

Rhodofactory 2012

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