Team:Tec-Monterrey/allergen/notebook

From 2012.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 231: Line 231:
<script type="text/javascript">
<script type="text/javascript">
$(document).ready(function(){
$(document).ready(function(){
-
alert("never read");
 
$("#div_subcontent").hide();
$("#div_subcontent").hide();
$("#div_subcontent").fadeIn(1000);
$("#div_subcontent").fadeIn(1000);
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$("#div_subcontent").fadeOut(1000);
$("#div_subcontent").fadeOut(1000);
});
});
-
alert("???");
 
//Tabs
//Tabs
var month = 0;
var month = 0;
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var sep_type = new Array();
var sep_type = new Array();
var sep_info = new Array();
var sep_info = new Array();
-
alert("tabfunctions");
 
$('#tab1').click(function() {
$('#tab1').click(function() {
month = 0;
month = 0;
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toggleMonth();
toggleMonth();
});
});
-
alert("buttongs")
 
//notebook buttons
//notebook buttons
$('#btd1').click(function() { replaceBox(1); });
$('#btd1').click(function() { replaceBox(1); });
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$('#btd30').click(function() { replaceBox(30); });
$('#btd30').click(function() { replaceBox(30); });
$('#btd31').click(function() { replaceBox(31); });
$('#btd31').click(function() { replaceBox(31); });
-
alert("funcs");
 
function toggleMonth()
function toggleMonth()
{
{
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function replaceBox(day)
function replaceBox(day)
{
{
-
if (month == 0 && day <= apr_info.length)
+
if (month == 0)
{
{
$('#imgbox').attr("src",all_images[apr_type[day-1]]);
$('#imgbox').attr("src",all_images[apr_type[day-1]]);
$('#textbox').html(apr_info[day-1]);
$('#textbox').html(apr_info[day-1]);
}
}
-
else if (month == 1 && day <= may_info.length)
+
else if (month == 1)
{
{
$('#imgbox').attr("src",all_images[may_type[day-1]]);
$('#imgbox').attr("src",all_images[may_type[day-1]]);
$('#textbox').html(may_info[day-1]);
$('#textbox').html(may_info[day-1]);
}
}
-
else if (month == 2 && day <= jun_info.length)
+
else if (month == 2)
{
{
$('#imgbox').attr("src",all_images[jun_type[day-1]]);
$('#imgbox').attr("src",all_images[jun_type[day-1]]);
$('#textbox').html(jun_info[day-1]);
$('#textbox').html(jun_info[day-1]);
}
}
-
else if (month == 3 && day <= jul_info.length)
+
else if (month == 3)
{
{
$('#imgbox').attr("src",all_images[jul_type[day-1]]);
$('#imgbox').attr("src",all_images[jul_type[day-1]]);
$('#textbox').html(jul_info[day-1]);
$('#textbox').html(jul_info[day-1]);
}
}
-
else if (month == 4 && day <= aug_info.length)
+
else if (month == 4)
{
{
$('#imgbox').attr("src",all_images[aug_type[day-1]]);
$('#imgbox').attr("src",all_images[aug_type[day-1]]);
$('#textbox').html(aug_info[day-1]);
$('#textbox').html(aug_info[day-1]);
}
}
-
else if (month == 5 && day <= sep_info.length)
+
else if (month == 5)
{
{
$('#imgbox').attr("src",all_images[sep_type[day-1]]);
$('#imgbox').attr("src",all_images[sep_type[day-1]]);
Line 693: Line 688:
aug_info[1] = "Stock from <i>Pichia Pastoris</i>";
aug_info[1] = "Stock from <i>Pichia Pastoris</i>";
aug_info[2] = "Dilutions<br /><br />Re-suspension from iGem Parts experiments for the improvement of parts";
aug_info[2] = "Dilutions<br /><br />Re-suspension from iGem Parts experiments for the improvement of parts";
-
aug_info[3] = "None";
+
aug_info[3] = "<u>Transformation</u> we had a Top10 stock and it resulted that had resistance to kanamycin and chloramphenicol, turned out to be Rossetta. <br /><br /><u>MIDIPREP for pPink HC</u><br /><br /><u>Electrophoresis</u> testing to find pPink HC , good results. <br /><br />";
-
aug_info[4] = "None";
+
aug_info[4] = "Competent cells of Top10F<br /><br />Re-suspension for Genescript’s lyophilized filter paper:  nanodrop (Z maize 63 ng/ul , antihis 64 ng/ul, derf 97ng/ul) <br /><br />Purification of electrophoresis gel and quantification of nanodrop<br /><br />Transformation of iGEM parts and Genescript";
-
aug_info[5] = "None";
+
aug_info[5] = "Dilutions<br /><br />2 Preinoculums Pichia Pastoris for electro and chemically competent cells";
-
aug_info[6] = "None";
+
aug_info[6] = "Chemically competent cells<br /><br />Dilutions<br /><br />Phone-calling to Sponsorships";
-
aug_info[7] = "None";
+
aug_info[7] = "Quantification of bacteria from dilutions";
-
aug_info[8] = "None";
+
aug_info[8] = "Dilutions";
-
aug_info[9] = "None";
+
aug_info[9] = "Transformation of His and new parts<br /><br />Quantification of bacteria from dilutions <br /><br />Development of Videogame<br /><br />Overnight Digestion<br /><br />DNA extraction from parts<br /><br />MIDIPREP for Pichia Pastoris transformation<br /><br />Stock from Pichia Pastoris";
-
aug_info[10] = "None";
+
aug_info[10] = "Medium preparation and sterilization<br /><br />Purification of digestion<br /><br />Transformation Pichia Pastoris with HIS<br /><br />Colony selection for GFP<br /><br />Electrophoresis of yesterday’s parts and digestion";
-
aug_info[11] = "None";
+
aug_info[11] = "Miniprep for His, BEE, GFP and AFP<br /><br />Digestion with AFIII of all of them<br /><br />Electrophoresis didn’t show results<br /><br />Stocks (His, BEE, GFP and AFP)";
-
aug_info[12] = "None";
+
aug_info[12] = "Miniprep of His, BEE, GFP, and AFP<br /><br />Confirmation by Electrophoresis gel<br /><br />Preinoculum<br /><br />Dilutions";
-
aug_info[13] = "None";
+
aug_info[13] = "Workshop #1 What is the material and equipment of a Lab and how to use it? <br /><br />Quantification of Bacteria (Dilutions) <br /><br />MIDIPREP <br /><br />Overnight Digestion for BEE and GFP";
-
aug_info[14] = "None";
+
aug_info[14] = "Selling Chocolates for funding<br /><br />Meet Sponsors from Accura<br /><br />Electrophoresis of Overnight digestion (BEE and GFP) <br /><br />Purification of DNA<br /><br />Transformation of derf in Rossetta<br /><br />Dilutions";
-
aug_info[15] = "None";
+
aug_info[15] = "Miniprep of derf, GFP and HIS<br /><br />Electrophoresis<br /><br />MIDIPREP<br /><br />Screening for transformed Rossetta<br /><br />Rossetta’s induction with IPTG<br /><br />AFP induction with Arabinose<br /><br />Stock from AFP, AFP2, HIS<br /><br />Quantification of bacteria (from dilutions)";
-
aug_info[16] = "None";
+
aug_info[16] = "Digestion GFPS and HIS<br /><br />Electrophoresis<br /><br />MIDIPREP for HIS<br /><br />Sponsor meetings<br /><br />Transformation Rosseta with DERF (since no results)";
-
aug_info[17] = "None";
+
aug_info[17] = "Nanodrop from the MIDIPREP<br /><br />Electrophoresis confirming GFP, AntiHIS and HIS<br /><br />Digestion<br /><br />Sterilization<br /><br />Preparation of Arabinose<br /><br />Precipitation by ETOH<br /><br />Transformation of Pichia Pastoris<br /><br />";
aug_info[18] = "None";
aug_info[18] = "None";
-
aug_info[19] = "None";
+
aug_info[19] = "Dilutions<br /><br />Preinoculum of TOP10F for miniprep";
-
aug_info[20] = "None";
+
aug_info[20] = "Workshop #2 (August 21st, 2012): Bacterial DNA: Purification of Plasmid DNA (Miniprep) <br /><br />Quantification of Bacteria (Dilutions) <br /><br />Pichia pastoris preinoculum<br /><br />Preparation of SDS page<br /><br />Preparation of Staining Protocol solutions";
-
aug_info[21] = "None";
+
aug_info[21] = "Pichia pastoris transformed growing in Minimum Media<br /><br />Quantification of induction<br /><br />SDS page wash and revealed, no results";
-
aug_info[22] = "None";
+
aug_info[22] = "Quantification<br /><br />Pichia Induction<br /><br />SDS-Page for DERF and AFP<br /><br />Miniprep DERF and AFP<br /><br />Results for AFP protein<br /><br />Dilutions";
-
aug_info[23] = "None";
+
aug_info[23] = "Induction<br /><br />Quantification of Bacteria (Dilutions)";
-
aug_info[24] = "None";
+
aug_info[24] = "Transformation Rossetta with AFP<br /><br />Induction for E.Coli and Pichia Pastoris (DERF, AFP) (DERF, Zea Mays, GFP, HIS)";
-
aug_info[25] = "None";
+
aug_info[25] = "Made Buffers (sample and lysis) <br /><br />Induction of Pichia Pastoris ";
-
aug_info[26] = "None";
+
aug_info[26] = "MIDIPREP for derf and Zea Mays<br /><br />Preparation of sample (soluble e insoluble parts) <br /><br />Sample recovery";
-
aug_info[27] = "None";
+
aug_info[27] = "Made SDS page gels (Triscine and glycine) ";
-
aug_info[28] = "None";
+
aug_info[28] = "Again Make SDS page gels (Triscine and glycine) <br /><br /> Make counting of viability of cells from AFP project";
-
aug_info[29] = "None";
+
aug_info[29] = "Digestion of Zm, Zm2, Derf<br /><br />DNA precipitation (Derf and Zm)";
aug_info[30] = "None";
aug_info[30] = "None";
aug_type[0] = 2;
aug_type[0] = 2;
-
aug_type[1] = 0;
+
aug_type[1] = 2;
-
aug_type[2] = 0;
+
aug_type[2] = 2;
-
aug_type[3] = 0;
+
aug_type[3] = 2;
-
aug_type[4] = 0;
+
aug_type[4] = 2;
-
aug_type[5] = 0;
+
aug_type[5] = 2;
-
aug_type[6] = 0;
+
aug_type[6] = 2;
-
aug_type[7] = 0;
+
aug_type[7] = 2;
-
aug_type[8] = 0;
+
aug_type[8] = 2;
-
aug_type[9] = 0;
+
aug_type[9] = 1;
-
aug_type[10] = 0;
+
aug_type[10] = 2;
-
aug_type[11] = 0;
+
aug_type[11] = 2;
-
aug_type[12] = 0;
+
aug_type[12] = 2;
-
aug_type[13] = 0;
+
aug_type[13] = 3;
-
aug_type[14] = 0;
+
aug_type[14] = 1;
-
aug_type[15] = 0;
+
aug_type[15] = 2;
-
aug_type[16] = 0;
+
aug_type[16] = 2;
-
aug_type[17] = 0;
+
aug_type[17] = 2;
aug_type[18] = 0;
aug_type[18] = 0;
-
aug_type[19] = 0;
+
aug_type[19] = 2;
aug_type[20] = 0;
aug_type[20] = 0;
-
aug_type[21] = 0;
+
aug_type[21] = 2;
-
aug_type[22] = 0;
+
aug_type[22] = 2;
-
aug_type[23] = 0;
+
aug_type[23] = 2;
-
aug_type[24] = 0;
+
aug_type[24] = 2;
-
aug_type[25] = 0;
+
aug_type[25] = 2;
-
aug_type[26] = 0;
+
aug_type[26] = 2;
-
aug_type[27] = 0;
+
aug_type[27] = 2;
-
aug_type[28] = 0;
+
aug_type[28] = 2;
-
aug_type[29] = 0;
+
aug_type[29] = 2;
aug_type[30] = 0;
aug_type[30] = 0;
Line 763: Line 758:
sep_info[6] = "None";
sep_info[6] = "None";
sep_info[7] = "None";
sep_info[7] = "None";
-
sep_info[8] = "None";
+
sep_info[8] = "Preinoculum of pichia pastoris (for transform Zm and Derf) <br /><br />Preinoculum  for miniprep of  GFP, His, Apim6, Derf, Zm<br /><br />Talk with Dr. Guy Cardinau <br /><br />Extraction of E. coli  samples (48°C and 72°C)";
-
sep_info[9] = "None";
+
sep_info[9] = "Workshop #3 (September 13th, 2012): Karyotype and Chromosomes";
-
sep_info[10] = "None";
+
sep_info[10] = "<u>SDS Preparation</u><br />Sample Preparation, Place gels in the supports and loading samples<br />Washing and staining gels (5) <br /><br /><u>DNA Preparation</u><br />Miniprep crop (Zea mays 2, 2 Derf) <br />Digestion corroborating (AflII) <br />Electrophoresis<br /><br /><u>Transformation</u> <br />Screening with digestion (EcoRI) and electrophoresis (transformation went wrong) <br /><br /><u>Pichia Pastoris Transformation</u><br />Inoculate virgin Pichia in YPD and incubate @ 30 ° C<br /><br /><u>Comprobatory Induction</u><br />Harvest total of 48 h of BL21-JM109-Ctrl ctrl &<br />Inoculation BBGM in 12 tubes with minimal media cultures bites GFP colonies, His & Apim6<br />Incubation @ 30 ° C<br /><br /><u>Activity for AFP</u><br />AFP pre-inoculum of TOP10, BL21 AFP AFP & JM109<br /><br /><u>Activity for GFP</u><br />Inoculation of two tubes with colonies BBGM GFP bites -Incubation @ 30 ° C<br /><br />";
-
sep_info[11] = "None";
+
sep_info[11] = "<u>SDS Preparation</u><br />Fade washing gels with 15 minutes. <br />Reveal gels (results were not conclusive) <br /><br /><u>DNA Preparation</u><br />Total DNA digestion overnight (Zea mays & Derf) with AflII<br /><u>Transformation</u><br />Transformation of BL21 & JM109 with Derf (transformation went wrong again) <br /><br />Xtractor of BL21-Ctrl-AFP BL21, JM109 & JM109-Ctrl-AFP (every 48 h) <br /><br /><u>Comprobatory Induction</u><br />Induction of bacteria (arabinose and NaCl) <br /><br />";
-
sep_info[12] = "None";
+
sep_info[12] = "<u>DNA Preparation</u><br />Corroborating Electrophoresis (decide whether it can be used to transform Pichia) (samples went wrong) <br />Inoculation for Miniprep of Zea mays & Derf<br /><br /><u>Transformation</u><br />Transformation of BL21 & JM109 with reliable DNA Derf <br />Inoculate virgin Pichia in YPD and incubate @ 30 ° C<br /><br /><u>Prepare SDS-PAGE gels</u><br />Mount gels and load samples (also RosDerf, TOP10 AFP). <br /> Prepare Samples with Sample Buffer<br />Wash, fixed and stained gels overnight<br /><br /><u>Induction of AFP</u><br />(TOP10, JM109, BL21) for SDS-PAGE and purification by column";
-
sep_info[13] = "None";
+
sep_info[13] = "<u>Miniprep Zea mays & Derf</u><br />Digestion corroborating (EcoRI) and electrophoresis. Check that they are well labeled.Overnight digestion (There was no evidence of plasmid) <br /><br /><u>Transformation of BL21 & JM109</u> <br />obtained from Miniprep Derf (transformed with DNA was not identified) <br /><br /><u>SDS PAGE</u><br />Wash with distilled water gels. 3 cycles of 5 minutes<br />Reveal gels<br /><br /><u>Research and planning of the experiment</u><br />Prepare 100 boxes of solid LB medium counts";
-
sep_info[14] = "None";
+
sep_info[14] = "Search reagents for silver staining<br /><br />Miniprep pPink-HC and possible Zea mays & Derf<br /><br />Digestion corroborating (HindIII) and electrophoresis. Check that they are well labeled. <br /><br />Overnight digestion (AflII) <br /><br />Transformation of BL21 & JM109 with the Miniprep Derf obtained and it has been proven (Transforms failed) <br /><br />Leave blank inoculum Pichia in YPD";
-
sep_info[15] = "None";
+
sep_info[15] = "Electrophoresis of digestion<br /><br />DNA Precipitation<br /><br />DNA Quantification<br /><br />Transformation of BL21 & JM109 with the Miniprep Derf obtained and it has been proven (Failed) <br /><br />Pichia Measure OD (OD600 = 1-2) <br /><br />Transformation with Derf<br /><br />Incubation @ 30 ° C in minimal medium boxes";
-
sep_info[16] = "None";
+
sep_info[16] = "Leavepre- inocule BL21 virgin & JM109<br /><br />Sterilize 2 flasks (with 50 mL of LB medium) <br /><br />Leave inoculum for Rosetta miniprep Derf<br /><br />Total Harvest cultures (5 mL). Centrifuge samples and store the supernatant and pellet separately. <br /><br />Induction of bacteria (arabinose and NaCl) in fresh LB medium (not grew preinóculos) <br /><br />Total Harvest cultures (5 mL). Centrifuge samples and store the supernatant and pellet separately. <br /><br />Total Harvest cultures of JM109<br /><br />Centrifuge samples and storing pellets. Make sample Xtractor<br /><br />Induction of TOP10 & BL21 virgins (0.4% & 0.6 M NaCl arabinose) <br /><br />Sample processing, and testing laboratories contacts necessary<br /><br />";
-
sep_info[17] = "None";
+
sep_info[17] = "<ul><li>Workshop #4 Restriction Enzymes and Electrophoresis</li><li>BL21 competent cells JM109 (Failed) </li><li>Check that we have to transform Derf</li><li>Leave cell preinoculum virgin BL21 & JM109</li><li>Induction evidencing Pichia with Zea mays & Derf</li><li>Total Harvest cultures (5 mL). Centrifuge samples and store the supernatant and pellet separately. </li><li>Concentrate samples with filters of 10 kDa</li><li>Prepare and run one gel for SDS-PAGE (12% Tricine) for GFP, His & Ctrl</li><li>Wash, fixed and stained gels overnight</li><li>Growth of bacterial cultures of 0.1 to 0.6</li><li>Induction of bacteria (arabinose and NaCl) into fresh LB medium (Failed) </li><li>Leave preinoculum of TOP10 & JM109 (AFP & Ctrl) </li><li>Total Harvest cultures (5 mL). Centrifuge samples and store the supernatant and pellet separately. </li><li>Concentrate samples with filters of 10 kDa</li><li>Prepare and run one gel for SDS-PAGE (12% Tricine) for GFP, His & Ctrl</li><li>Wash, fixed and stained gels overnight</li><li>Harvest cultures partial BL21 & TOP10</li><li>Centrifuge samples and store pellets</li></ul>";
-
sep_info[18] = "None";
+
sep_info[18] = "<ul><li>Make silver staining</li><li>Reveal gels</li><li>Derf induction (Rosetta, JM109, BL21) for SDS-PAGE and purification by column</li><li>Growing crops (BL21 & JM109) in flasks OD600 = 0.1 to 0.6</li><li>Prepare & JM109 competent BL21</li><li>Transform JM109 & BL21 with Derf</li><li>Inoculate YPD medium with Pichia virgin Zea mays</li><li>Search DNA of Zea mays and let inocula for minipreps</li><li>Derf transformation colonies (6 each) and inoculating tubes MMBG (20 mL) </li><li>Wash with distilled water gels. Cycles 5 minutes. reveal gels</li><li>Apply this silver stain gel</li><li>Growth of bacterial cultures of 0.1 to 0.6</li><li>Induction of bacteria (arabinose and NaCl) into fresh LB medium</li><li>Wash with distilled water gels. 3 cycles of 5 minutes</li><li>Reveal gels</li><li>Xtractor lysing pellets by using lyticase. Soluble and insoluble phase store @ -20 ° C</li><li>Total Harvest culture BL21 & TOP10</li><li>Centrifuge samples and store pellets</li></ul>";
-
sep_info[19] = "None";
+
sep_info[19] = "Workshop #5 Applications of Restriction Enzymes and Electrophoresis<br /><br />Since the crops are growing colonies transformation chopped (cell below), leaving six tubes inocula in 15 mL of LB (Rosetta, BL21, JM109 - all Derf & ctrl) <br /<br />Chop colonies and incubate in LB (Amp) <br /><br />Inoculate tubes with 15 mL of fresh LB medium (Amp) with the above culture to Miniprep<br /><br />Inoculate tubes of minimal medium from the cultures (Derf) induction of BBGM<br /><br />Miniprep DNA digestion and Zea mays. (Lack electrophoresis)";
-
sep_info[20] = "None";
+
sep_info[20] = "Growth of bacteria in 100 mL flasks with LB (50 mL controls), 0.1-0.6<br /><br />Induction with 0.5 mM IPTG<br /><br />Centrifuge cells and move to new LB medium (100 mL or 50 mL) <br /><br />Incubation @ 16 ° C overnight<br /><br />Miniprep chopped colonies<br /><br />Corroborating digestion (EcoRI) <br /><br />Electrophoresis<br /><br />Stocks of colonies transformed with Derf<br /><br />Make glycerol stocks with 20% Derf<br /><br />Transform Pichia with Zea mays<br /><br />Incubation @ 30 ° C in minimal medium boxes<br /><br />Pass Medium crops methanol (5 ml per tube) <br /><br />";
-
sep_info[21] = "None";
+
sep_info[21] = "Total Harvest cultures (20 mL) <br /><br />Centrifuge samples and store the supernatant and pellet separately<br /><br />Xtractor lysing pellets by using lyticase. Soluble and insoluble phase store @ -20 ° C<br /><br />Preparing samples for purification material and columns of the His-tag protein";
-
sep_info[22] = "None";
+
sep_info[22] = "Prepare Samples with Sample Buffer<br /><br />Prepare SDS-PAGE gels<br /><br />Mount gels and load samples. Running @ 90 V, 2.00 A<br /><br />Wash, fixed and stained gels overnight<br /><br />Purify His-tag protein by columns. Analyze results";
-
sep_info[23] = "None";
+
sep_info[23] = "Made execution of AFP experiment <br /><br />Made SDS gels <br /><br />Preparing samples for purification material and columns of the His-tag protein";
sep_info[24] = "None";
sep_info[24] = "None";
-
sep_info[25] = "None";
+
sep_info[25] = "Wiki Freeze<br /><br />Activity assay for anti-IgE and anti-His, also activity assay for shuttle sequence in Pichia pink";
sep_info[26] = "None";
sep_info[26] = "None";
sep_info[27] = "None";
sep_info[27] = "None";
Line 794: Line 789:
sep_type[6] = 0;
sep_type[6] = 0;
sep_type[7] = 0;
sep_type[7] = 0;
-
sep_type[8] = 0;
+
sep_type[8] = 2;
-
sep_type[9] = 0;
+
sep_type[9] = 3;
-
sep_type[10] = 0;
+
sep_type[10] = 2;
-
sep_type[11] = 0;
+
sep_type[11] = 2;
-
sep_type[12] = 0;
+
sep_type[12] = 2;
-
sep_type[13] = 0;
+
sep_type[13] = 2;
-
sep_type[14] = 0;
+
sep_type[14] = 2;
-
sep_type[15] = 0;
+
sep_type[15] = 2;
-
sep_type[16] = 0;
+
sep_type[16] = 3;
-
sep_type[17] = 0;
+
sep_type[17] = 2;
-
sep_type[18] = 0;
+
sep_type[18] = 2;
-
sep_type[19] = 0;
+
sep_type[19] = 3;
-
sep_type[20] = 0;
+
sep_type[20] = 2;
-
sep_type[21] = 0;
+
sep_type[21] = 2;
-
sep_type[22] = 0;
+
sep_type[22] = 2;
-
sep_type[23] = 0;
+
sep_type[23] = 2;
sep_type[24] = 0;
sep_type[24] = 0;
-
sep_type[25] = 0;
+
sep_type[25] = 3;
sep_type[26] = 0;
sep_type[26] = 0;
sep_type[27] = 0;
sep_type[27] = 0;
Line 853: Line 848:
<table>
<table>
<tr>
<tr>
-
<td align="right"><button type="button" id="sp1" onclick="document.location.href='https://2012.igem.org/Main_Page'">&nbsp;</button></td>
+
<td align="right">
 +
<img src="https://static.igem.org/mediawiki/2012/d/d6/TECMTY_BANNER_SMALL.JPG" />
 +
<button type="button" id="sp1" onclick="document.location.href='https://2012.igem.org/Main_Page'">&nbsp;</button>
 +
</td>
</tr>
</tr>
<tr>
<tr>
Line 869: Line 867:
<td align="center" width="167px">
<td align="center" width="167px">
<button type="button" id="cl1">&nbsp;</button>
<button type="button" id="cl1">&nbsp;</button>
-
</td>
 
-
<td align="center" width="167px">
 
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<button type="button" id="cl2">&nbsp;</button>
 
</td>
</td>
<td align="center" width="167px">
<td align="center" width="167px">
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<td>
<td>
<div id="textbox">
<div id="textbox">
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad mit in culpa qui officia deserunt mollit anim id est laborum
+
<noscript>
 +
Enable Javascript to view
 +
</noscript>
</div>
</div>
</td>
</td>

Latest revision as of 03:29, 26 October 2012

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project
imgbox

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