Team:Michigan/Notebook

From 2012.igem.org

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[[Team:Michigan/Notebook/Protocols]]
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{| style="color:#ffffff;background-color:#929487;" cellpadding="3" cellspacing="1" border="0" width="100%" align="center"
!align="center"|[[Team:Michigan|Home]]
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!align="center"|[[Team:Michigan/Attributions|Attributions]]
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<!-- *** Here is where you should enter your lab notebook! Remember to name and date your entries. Use the icons on the top left corner to embed image, bold, italicize, and so forth.  For technical support, email huetone@gmail.com or ask Candy. *** -->
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    <p><a href="/Team:Michigan/Project">Project</a> > <a href="/Team:Michigan/Noteboook">Notebook</a></p>
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<h2>Modify HbiF into a Biobrick compatible format </h2>
 
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<p>HbiF and LVA degradation tag were ordered from IDT with the following specifications.</p>
 
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">EcoRI</span></p>
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<p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p>
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<p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p>
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">AgeI</span></p>
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">NgoMIV</span></p>
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<p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :<br/></p>
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<h2>Cloning Team Notebook</h2>
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<iframe width=100% height=800; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&amp;embedded=true"></iframe>
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<p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p>
 
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<h2>Prepare FimE for protein expression</h2>
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<iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&amp;embedded=true"></iframe>
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<h2>Team Reports</h2>
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<iframe src="https://docs.google.com/presentation/embed?id=17czITWHCoyzbzdYCvXWmwpDbl31jJyhgNmb32IXX3aY&start=false&loop=false&delayms=8000" frameborder="0" width=60% height=400 allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe>
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Latest revision as of 02:36, 4 October 2012

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Cloning Team Notebook