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Team:Evry-Genopole/Notebook/July/2
From 2012.igem.org
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1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before <br> | 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before <br> | ||
| - | 2) Transformed bacteria spreaded on Petri's plate- incubation 37 degrees Celsius, overnight <br> | + | 2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampiciline- incubation 37 degrees Celsius, overnight <br> |
| - | 3) SOC preparation: | + | 3) SOC preparation: <br> |
1,4g Hanahan's Broth | 1,4g Hanahan's Broth | ||
0,18g Glucose | 0,18g Glucose | ||
50mL MiliQ water | 50mL MiliQ water | ||
filtrated on microfilter (if you have more important volume, autoclave it) | filtrated on microfilter (if you have more important volume, autoclave it) | ||
Latest revision as of 16:00, 2 July 2012
Today's achievements:
1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before
2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampiciline- incubation 37 degrees Celsius, overnight
3) SOC preparation:
1,4g Hanahan's Broth
0,18g Glucose
50mL MiliQ water
filtrated on microfilter (if you have more important volume, autoclave it)
