Team:WashU/Protocols/GlycerolStock

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=General Glycerol Stock Protocol=
=General Glycerol Stock Protocol=
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1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
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2) If possible, flash freeze in dry ice bathed in 70% ethanol.
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
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3) Store at -80°C.
3) Store at -80°C.
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[https://2012.igem.org/Team:WashU/Protocols Back to Protocols]

Latest revision as of 21:32, 9 August 2012


General Glycerol Stock Protocol


1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
3) Store at -80°C.

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