Team:LMU-Munich

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
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|<font size="5">'''Opening ''Bacillus subtilis'' and its unique biology to the iGEM world'''</font>
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[[File:Prezi Home.png|600px|link=Team:LMU-Munich/Project]]
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|[[File:NEXT.png|100px|link=Team:LMU-Munich/Abstract]]
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|'''Bead'''zillus - For a guided tour through the most important information about our project and results, klick [[Team:LMU-Munich/Abstract|here]].
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
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|Why is there a need for our Project [[Team:LMU-Munich/Why_Beadzillus|'''Bead'''zillus]]?
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
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|[[File:LMU_The_solution.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
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|[[File:Requirements.png|95px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].
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|[[File:4-Sporobeads.png|95px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|Finally, we succeeded in displaying proteins on endospores. Our [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]].
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|[[File:LMU 5-Application.png|90px|link=Team:LMU-Munich/Application]]
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] are imaginable.
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==='''Bead'''zillus: Fundamental BioBricks for ''Bacillus subtilis'' and spores as a platform for protein display===
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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We chose to work with [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction <i>Bacillus subtilis</i>] to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox] (<b>B<sup>4</sup></b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress-resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>B<sup>4</sup></b>, we developed [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins <b>Sporo</b>beads]. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector <b>Sporo</b>vector] to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we [https://2012.igem.org/Team:LMU-Munich/Germination_Stop prevented germination] by knocking out involved genes and developed a [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Suicide</b>switch] turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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|[[Image:LMU-Munich_logo.png|200px|right|frame]]
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|[[Image:LMU-Munich_team.png|right|frame|Your team picture]]
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====Team LMU Munich 2012====
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors. Visit our [https://2012.igem.org/Team:LMU-Munich/Team Team] page for more Details.
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|align="center"|[[Team:LMU-Munich | Team LMU-Munich]]
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<a href="http://www.en.uni-muenchen.de/index.html">
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<a href="http://www.biologie.uni-muenchen.de/index.html">
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<img src="https://static.igem.org/mediawiki/2012/4/48/Biozentrum_LMU.jpg" height="170" border=0 /></a></html>
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BEADzillus
 
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We want to create Bacillus subtilis spores with different hybrid proteins on the outmost spore coat layer for multiple purposes.
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As an safety issue, we want to disable the spores from germination.
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<a href="https://2012.igem.org/Team:LMU-Munich/Team">
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To provide the partsregistry with thoroughly evaluted biobricks we want to adapt B. subtilis vectors compatible for the BioBrick system and characterize different promoters.
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<!--- The Mission, Experiments --->
 
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[[File:Uumail.png|80px|left]]
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!align="center"|[[Team:LMU-Munich|Home]]
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Email us!
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!align="center"|[[Team:LMU-Munich/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=LMU-Munich Official Team Profile]
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igem@bio.lmu.de
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!align="center"|[[Team:LMU-Munich/Project|Project]]
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!align="center"|[[Team:LMU-Munich/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:LMU-Munich/Modeling|Modeling]]
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!align="center"|[[Team:LMU-Munich/Notebook|Notebook]]
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[[File:Facebook logo amsterdam.png|70px|left|link=https://www.facebook.com/igem.lmu]] Visit us on facebook
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!align="center"|[[Team:LMU-Munich/Safety|Safety]]
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!align="center"|[[Team:LMU-Munich/Attributions|Attributions]]
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[[File:Exe12_Youtubelogo.png|65px|left|link=http://www.youtube.com/user/igemLMUmunich]] Check out our videos!
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| [[Team:LMU-Munich/Team|Find out who we are!]]      
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====Our Gold Sponsors====
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<a href="https://2012.igem.org/Team:LMU-Munich/Sponsors">
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Latest revision as of 13:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich"