"
Team:LMU-Munich/Data/Inverter
From 2012.igem.org
| (One intermediate revision not shown) | |||
| Line 20: | Line 20: | ||
|} | |} | ||
| - | The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uof<sub>CGU</sub> and masks the Shine Dalgarno sequence, thereby translationally repressing it. Uof<sub>CGU</sub> is translationally fused to ''lacZα'', and therefore also translationally repressed. Uof<sub>CGU</sub>-''lacZα'' is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The P<sub>''BAD''</sub> is titrated with arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the ''lacZα'' translational repression. | + | <p align="justify">The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uof<sub>CGU</sub> and masks the Shine Dalgarno sequence, thereby translationally repressing it. Uof<sub>CGU</sub> is translationally fused to ''lacZα'', and therefore also translationally repressed. Uof<sub>CGU</sub>-''lacZα'' is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The P<sub>''BAD''</sub> is titrated with arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the ''lacZα'' translational repression.</p> |
| + | |||
| + | |||
| + | |||
| + | |||
| + | [[File:Arrow purple Data.png|center|100px|link=Team:LMU-Munich/Data]] | ||
| + | |||
| + | |||
| + | |||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Latest revision as of 01:59, 27 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
β-galoctisidase assay of the Inverter with lacZα
|
The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uofCGU and masks the Shine Dalgarno sequence, thereby translationally repressing it. UofCGU is translationally fused to lacZα, and therefore also translationally repressed. UofCGU-lacZα is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The PBAD is titrated with arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the lacZα translational repression.
