Team/CINVESTAV-IPN-UNAM MX/Overview.htm
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- | </ul | + | <ul> |
- | + | <li><a href="home.htm">Home</a></li> | |
+ | <li class="active"><a href="rhodofactory.htm">Rhodofactory</a></li> | ||
+ | <li><a href="promoterselection.htm">Promoter selection </a></li> | ||
+ | <li><a href="results.htm">results </a></li> | ||
+ | <li><a href="perspectives.htm">Perspectives</a></li> | ||
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+ | <li><a href="aboutus.htm">About us</a> </li> | ||
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+ | <div id="page"> | ||
+ | <div id="context"> | ||
+ | |||
+ | <h1><em>Overview! </em></h1> | ||
+ | <p>The purple non-sulfur photosynthetic bacteria (PNSB) belong to the alpha-proteobacteria group, because of their genetic regulatory systems | ||
+ | which coordinate different metabolic states, these microorganisms are able to grow under a wide variety of environmental conditions (1).<br><br> | ||
+ | |||
+ | Specifically, our project aims to isolate two genetic control systems based on <em>Rhodobacter sphaeroides</em> photosynthesis cluster regulation. The first one is the | ||
+ | oxygen dependant system PrrA/PrrB, when oxygen tension is high it remains inactive, and when the oxygen is low it activates gene expression | ||
+ | (2). The second system is the light and oxygen mediated system AppA/PpsR that represses gene expression under aerobic conditions and allows | ||
+ | transcription in the absence of oxygen and presence light (3).<br><br> | ||
+ | |||
+ | To achieve this goal we designed two genetic biobrick in which GFP expression is oxygen and light-dependent by the antirepression of PpsR and | ||
+ | oxygen dependent by the activation of PrrA/B system. The lab work is accompanied by a computational model, which will provide a way of | ||
+ | testing our knowledge of these systems.<br><br> | ||
+ | |||
+ | Once, we have characterized the functionality of these regulatory systems we aim to take advantage of <em>Rhodopseudomonas palustris’</em> metabolic versatility, and use | ||
+ | this bacteria as a microbial factory, that could work for the production of metabolites with economic value products using CO2 as carbon source.<br><br> | ||
+ | |||
+ | We are planning to use the S04147 clostridial butanol production operon (University of Alberta iGEM Team 2007) to evaluate the synthesis of | ||
+ | this biofuel, linking it to our control systems. This would provide an interesting way to produce butanol using CO2 as carbon source under | ||
+ | anaerobic photosynthetic conditions.</p> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/2012/c/ce/Rodo01.jpg" alt="rodo01" width="559" height="437"><br> | ||
+ | <br> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div id="sidebar"> | ||
+ | <div id="updates" class="orangebox"> | ||
+ | <h2>Rhodofactory</h2> | ||
+ | <ul> | ||
+ | <li><a href="Overview.htm" target="_parent">Overview</a></li> | ||
+ | <li><a href="Light_Response.htm">Light and Oxygen</a></li> | ||
+ | <li><a href="Oxigen_Response.htm">Oxygen Response</a></li> | ||
+ | <li><a href="Chassis.htm">Chassis</a></li> | ||
+ | </ul> | ||
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- | + | </div> | |
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- | + | <div id="piedepag"> | |
- | + | <p align="center"> <strong>Rhodofactory 2012</strong> </p> | |
- | + | <div id="sponsors"> | |
- | </ | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div> |
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- | <div id=" | + | <div id="sponsors"> |
- | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/6/67/Osli.png" alt="osli" width="88" height="46" /></div> | |
- | + | </div> | |
- | + | ||
- | <div id=" | + | <div id="sponsors"> |
- | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="80" height="97" /></div> | |
- | + | </div> | |
- | + | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div> | |
- | + | <div id="sponsors"> | |
- | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height="91" /></div> | |
- | + | </div> | |
- | + | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div> | |
- | + | <div id="sponsors"> | |
- | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div> | |
- | + | </div> | |
- | + | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div> | |
- | + | <div id="sponsors"> | |
- | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div> | |
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- | <div | + | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div> |
+ | <div id="sponsors"> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="70" height="96" /></div> | ||
+ | </div> | ||
+ | |||
</div> | </div> | ||
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Latest revision as of 03:30, 27 October 2012
Overview!
The purple non-sulfur photosynthetic bacteria (PNSB) belong to the alpha-proteobacteria group, because of their genetic regulatory systems
which coordinate different metabolic states, these microorganisms are able to grow under a wide variety of environmental conditions (1).
Specifically, our project aims to isolate two genetic control systems based on Rhodobacter sphaeroides photosynthesis cluster regulation. The first one is the
oxygen dependant system PrrA/PrrB, when oxygen tension is high it remains inactive, and when the oxygen is low it activates gene expression
(2). The second system is the light and oxygen mediated system AppA/PpsR that represses gene expression under aerobic conditions and allows
transcription in the absence of oxygen and presence light (3).
To achieve this goal we designed two genetic biobrick in which GFP expression is oxygen and light-dependent by the antirepression of PpsR and
oxygen dependent by the activation of PrrA/B system. The lab work is accompanied by a computational model, which will provide a way of
testing our knowledge of these systems.
Once, we have characterized the functionality of these regulatory systems we aim to take advantage of Rhodopseudomonas palustris’ metabolic versatility, and use
this bacteria as a microbial factory, that could work for the production of metabolites with economic value products using CO2 as carbon source.
We are planning to use the S04147 clostridial butanol production operon (University of Alberta iGEM Team 2007) to evaluate the synthesis of
this biofuel, linking it to our control systems. This would provide an interesting way to produce butanol using CO2 as carbon source under
anaerobic photosynthetic conditions.
![rodo01](https://static.igem.org/mediawiki/2012/c/ce/Rodo01.jpg)
Rhodofactory 2012
![icytdf](https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png)
![osli](https://static.igem.org/mediawiki/2012/6/67/Osli.png)
![bio](https://static.igem.org/mediawiki/2012/f/fb/Bio.png)
![fermentAS](https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png)
![cinestav](https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png)
![genscript](https://static.igem.org/mediawiki/2012/9/9b/Genscript.png)
![unam](https://static.igem.org/mediawiki/2012/1/16/Unam.png)
![gto](https://static.igem.org/mediawiki/2012/c/c9/Gto.png)
![quimica](https://static.igem.org/mediawiki/2012/f/fa/Qimica.png)
![valaner](https://static.igem.org/mediawiki/2012/7/73/Valaner.png)
![ipn](https://static.igem.org/mediawiki/2012/7/79/Ipn.png)