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<h1><em>Rhodofactory, controlling genetic expression: an oxygen <br /><br />and light response!</em></h1>
 +
<img src="https://static.igem.org/mediawiki/2012/e/ec/Mascota.jpg" width="290" height="287" class="left">
 +
<p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light,
 +
darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking
 +
advantage of this, our project aims to build two genetic control systems based on <em> Rhodobacter sphaeroides </em>
 +
photosynthesis cluster regulation. The first one is a light dependent system controlled by two
 +
proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is
 +
an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on
 +
R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions
 +
that activate or repress its expresion. Once we have characterized the functionality of these
 +
networks, our perspective is to develop a Rhodofactory, it means to control the produccion of
 +
differents metabolites, such as biodiesel and butanol, using simple signals.</p>
 +
<div style="clear: both;">&nbsp;</div>
 +
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<!-- end #page -->
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<div id="piedepagina">
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<p align="center"> <strong>Rhodofactory 2012</strong></p>
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<div id="sponsors">
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  <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div>
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  <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="80" height="97" /></div>
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      <img src="https://static.igem.org/mediawiki/2012/f/f3/RHODOFACTORY_rojo-09.fw.png" width="199" height="279" /> </div>
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<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div>
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-
      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis cluster regulation. </p>
+
</div>
-
      <p>The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on <em>R. palustris</em> chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.</p>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
-
      <p> Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals. </p>
+
<div id="sponsors">
-
     </div>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div>
 +
</div>
 +
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div>
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     <div id="sponsors">
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      <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div>
     </div>
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<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div>
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  <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="70" height="96" /></div>
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Latest revision as of 00:20, 27 October 2012

Rho

Rhodofactory, controlling genetic expression: an oxygen

and light response!

The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on Rhodobacter sphaeroides photosynthesis cluster regulation. The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion. Once we have characterized the functionality of these networks, our perspective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.

 

Rhodofactory 2012

icytdf
osli
bio
fermentAS
cinestav
genscript
unam
gto
quimica
valaner
ipn

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